Publication: Synthesis and characterization of chitosan-PVA hydrogel containing PEGylated recombinant epidermal growth factor on cell culture for wound healing substitute
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Date
2022-08-01
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Abstract
The aim of the current study was to assess the physicochemical characteristics and wound
healing activity of chitosan-polyvinyl alcohol (PVA) crosslinked hydrogel containing
recombinant human epidermal growth factor (rh-EGF) or recombinant mouse epidermal growth
factor (rm-EGF). The hydrogels were prepared and analyses were made of the morphological
properties, viscosity, water absorption capacity, mechanical and bio-adhesive properties. The
viscosity of the formulations varied between 14.400 - 48.500 cPs, with the greatest viscosity
values determined in K2 formulation. F2 formulation showed the highest water absorption
capacity. According to the studies of the mechanical properties, H2 formulation (0.153±0.018
N.mm) showed the greatest adhesiveness and E2 (0.245±0.001 mj/cm2
) formulation, the highest
bio-adhesion values. Hydrogels were cytocompatible considering in vitro cell viability values
of over 76% on human keratinocyte cells (HaCaT, CVCL-0038) and of over 84% on human
fibroblast cells (NIH 3T3, CRL-1658) used as a model cell line. According to the BrdU cell
proliferation results, B1 (197.82±2.48%) formulation showed the greatest NIH 3T3 and C1
(167.43±5.89%) formulation exhibited the highest HaCaT cell proliferation ability. In addition,
the scratch closure assay was performed to assess the wound healing efficiency of formulation
and the results obtained in the study showed that F2 formulation including PEGylated rh-EGF
had a highly effective role.
Keywords: Chitosan. rh-EGF. Hydrogel. Texture analyzer. Cell culture.
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Keywords
Sağlık Bilimleri, Eczacılık, Eczacılık Teknolojisi, Health Sciences, Pharmacology and Therapeutics, Pharmaceutics Technology, Klinik Tıp (MED), Yaşam Bilimleri (LIFE), Farmakoloji ve Toksikoloji, FARMAKOLOJİ VE ECZACILIK, Clinical Medicine (MED), Life Sciences (LIFE), PHARMACOLOGY & TOXICOLOGY, PHARMACOLOGY & PHARMACY, Farmakoloji, Farmakoloji, Toksikoloji ve Eczacılık (çeşitli), Genel Farmakoloji, Toksikoloji ve Eczacılık, Farmakoloji (tıbbi), İlaç Rehberleri, Yaşam Bilimleri, Pharmacy, Pharmacology, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), General Pharmacology, Toxicology and Pharmaceutics, Pharmacology (medical), Drug Guides, Life Sciences, Chitosan. rh-EGF. Hydrogel. Texture analyzer. Cell culture
Citation
Doğan M., Şahbaz S., Uğurlu T., Sezer A. D., "Synthesis and characterization of chitosan-PVA hydrogel containing PEGylated recombinant epidermal growth factor on cell culture for wound healing substitute", BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.58, ss.1-19, 2022