Person: ULAĞ, SONGÜL
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ULAĞ
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SONGÜL
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Publication Open Access Investigation of the properties of encapsulated hydrophilic and hydrophobic drugs in whey protein microparticles(2022-10-01) ULAĞ, SONGÜL; GÜNDÜZ, OĞUZHAN; Irem Deniz K., Ulağ S., Gündüz O.© 2022 Elsevier B.V.In this study, the amoxicillin (AMX) and rifampicin (RIF) were loaded into the whey protein (WP) to form the hydrophilic and hydrophobic drugs loaded whey protein microparticles. Scanning electron microscopy (SEM) images demonstrated that AMX addition increased the particle size, but RIF addition decreased the dimensions of the particles. The Fourier-transformed infrared spectroscopy (FTIR) results showed that both drugs were successfully loaded into the protein. AMX and RIF were ultimately released from the WP microparticles after 1440 min, according to the drug release test. Two drugs exhibited the same behaviour, but in the first hour, AMX had more release than the RIF.Publication Metadata only Fabrication, characterization and investigation of antibacterial activity of propolis substituted sodium alginate tissue scaffolds using three-dimensional (3d) printing technology(2021-06-05) UZUN, MUHAMMET; SU TORUN, SENA; ULAĞ, SONGÜL; AKSU, MEHMET BURAK; GÜNDÜZ, OĞUZHAN; CESUR, SÜMEYYE; Aarancı K., Uzun M., Su Torun S., Cesur S., Ulağ S., Amin A., Güncü M. M., Aksu M. B., Kolaylı S., Silva J., et al.Publication Open Access Fabrication of gentamicin sulfate-loaded 3d-printed polyvinyl alcohol/sodium alginate/gelatin-methacryloyl hybrid scaffolds for skin tissue replacement(2023-01-01) ULAĞ, SONGÜL; ŞAHİN, ALİ; AKSU, MEHMET BURAK; GÜNDÜZ, OĞUZHAN; Izgordu M. S., Ayran M., ULAĞ S., Yildirim R., Bulut B., ŞAHİN A., Guncu M. M., AKSU M. B., GÜNDÜZ O.3D-printed scaffolds can better mimic the function of human skin, both biologically and mechanically. Within the scope of this study, the effect of the addition of different amounts (10, 15, 20 mg) of gentamicin sulfate (GS) to a 10 mL solution of natural and synthetic polymers is investigated. Sodium alginate (SA), gelatin-methacryloyl (GelMA), and polyvinyl alcohol (PVA) are chosen as bioactive materials. The surface morphology and pore structures are visualized by scanning electron microscopy (SEM). According to the results, it is observed that the pore sizes of all scaffolds are smaller than 270 µm, the lowest value (130 µm) is obtained in the scaffold loaded with 15 mg GS, and it also has the highest tensile strength value (12.5 ± 7.6 MPa). Similarly, it is observed that the tensile strength (9.7 ± 4.5 MPa) is high in scaffold loaded with 20 mg GS. The biocompatibility test is performed with fibroblast cells, and the results show that the scaffolds are biocompatible with cells. The antibacterial test is carried out against the S.aureous and E. coli and the results indicate that all GS-loaded scaffolds demonstrate antibacterial activity.Publication Metadata only Investigation of Antimicrobial and Biofilm Properties of 3D Alginate/PolyethyleneGlycol/Phenethyl Alcohol Scaffolds.(2022-11-26) ULAĞ, SONGÜL; GÜNDÜZ, OĞUZHAN; TINAZ, GÜLGÜN; Aslan S. N., Altan E., ULAĞ S., GÜNDÜZ O., TINAZ G.Publication Open Access Controlled Release of Gentamicin from Electrospun Poly(Vinyl Alcohol)/Gelatin Nanofibers: The Effect of Crosslinking Time Using Glutaraldehyde Vapor(2023-02-03) ŞENGÖR, MUSTAFA; GÜNDÜZ, OĞUZHAN; ULAĞ, SONGÜL; Baykara D., Pilavci E., Cesur S., Ilhan E., ULAĞ S., ŞENGÖR M., Kijeńska-Gawrońska E., GÜNDÜZ O.© 2023 Wiley-VCH GmbH.In this study, polyvinyl alcohol (PVA), gelatin (GEL) and gentamicin (GEN) were used and 13PVA/0.5GEL/GEN nanofibers were fabricated with the electrospinning method. These nanofibers were crosslinked chemically with glutaraldehyde (GA) at different time intervals (2, 3, 4, 5, 5.5 and 6 h) to observe the crosslinking effect on the properties of the nanofibers. Morphological analysis reported that as the crosslinking time increased, the nanofiber diameters also increased from 369.26 nm (non-crosslinked) to 447.72 nm (6 h/crosslinking), respectively. The thermal characterization results demonstrated that crosslinking with different times noticeably shifted the thermal points.The tensile testing results proved that application of crosslinking enhanced the mechanical strength of the nanofibers from 3.31 MPa (non-crosslinked) to 5.8 MPa (5.5 h/crosslinking), respectively. The GEN release profiles from the nanofibers showed similar behaviors under crosslinking and indicated that the crosslinking time did not have a significant effect on the amount of the GEN released.Publication Open Access Fabrication of Amphotericin-B-loaded Sodium Alginate Nanoparticles for Biomedical Applications(2022-12-01) ULAĞ, SONGÜL; ŞENGÖR, MUSTAFA; GÜNDÜZ, OĞUZHAN; ULAĞ S., Celik S. E., ŞENGÖR M., GÜNDÜZ O.In this study, amphotericin-B (AMB)-loaded sodium alginate (SA) nanoparticles were fabricated using the electrospraying technique for biomedical applications. AMB is an antifungal agent and is poorly absorbed from the gastrointestinal tract due to its low aqueous solubility. Therefore, it should be given parenterally to treat systemic fungal infections. This study aims to transport it with nanoparticle formulations and observe the nanoparticle release behaviours. Scanning electron microscopy (SEM) images showed that nanoparticles of 0.5% SA fabricated at 37 kV had the most suitable particle diameter (93.36±24.386 nm) for loading 0.5, 1, and 3 ml of AMB. Fourier transform infrared spectroscopy (FTIR) results demonstrated that AMB successfully loaded into 0.5% SA nanoparticles. Drug release behaviours of the AMB-loaded particles indicated that AMB was released with a burst at the beginning, and release behaviour became sustainable after half an hour. The encapsulation efciencies of the diferent amounts of drug were calculated, and the results showed that the highest encapsulation efciency belonged to the 0.5% SA/1 AMB nanoparticles (42±1.23%).Publication Open Access Microfluidic systems for neural tissue engineering(Elsevier Science, Oxford/Amsterdam , 2023-01-01) CESUR, SÜMEYYE; ULAĞ, SONGÜL; GÜNDÜZ, OĞUZHAN; Cesur S., Ulağ S., Gündüz O.Damage to the nervous system due to illness or injury can cause serious and lasting loss of function or even fatal consequences. It is necessary to develop new treatment strategies to restore the function of the damaged nervous system.The optimal environment for nerve cell proliferation and differentiation is provided by neural tissue engineering. It aims to improve a new approximation for the therapy of nervous system diseases. Compared to 2D cell culture techniques, 3D cell culture systems ensure a more biomimetic environment and encourage more differentiation of cells. However, certain cell culture parameters have limitations in spatio-temporal control. With the advent of microfluidic systems, it can control the spatio-temporal dispersion of physical and chemical signals at the cellular level. In this section, microfluidic systems are explored as a tool to target both physical and chemical injury and recreate the post-injury environment, to study nerve injury at the cellular grade.Publication Open Access Evaluation of Diced Cartilage Grafts Shaped with Three-Dimensionally-Printed Bioresorbable Polycaprolactone Molds(2022-10-01) GÜNDÜZ, OĞUZHAN; ULAĞ, SONGÜL; Canli M., Karasoy Yeşilada A., ULAĞ S., Dobral A., Yalçin Ö., GÜNDÜZ O.Copyright © 2022 by the American Society of Plastic Surgeons.BACKGROUND: The main problem with the use of diced cartilage grafts is related to the difficulties encountered in shaping the graft and unpredictible graft resorption. The aim of this study was to evaluate the permanence and viability of diced cartilage grafts shaped with the help of biodegradable, three-dimensionally-printed polycaprolactone molds. METHODS: Three groups were studied in each of the eight rabbits: block cartilage (group 1), diced cartilage (group 2), and diced cartilage shaped with polycaprolactone molds (group 3). A total of 24 cartilage grafts were obtained at the end of the 12-week follow-up period, and 10 different histopathologic parameters were analyzed in each cartilage graft. RESULTS: Diced cartilages shaped with a three-dimensionally-printed polycaprolactone mold showed increased regeneration potential of chondrocytes, vascularization, and collagen production. Use of polycaprolactone molds did not cause any additional risk of inflammation, fibrosis, or metaplastic bone formation. CONCLUSIONS: In this study, it has been shown that three-dimensionally-printed polycaprolactone molds can be used safely in shaping diced cartilage grafts. In light of this study, it will be possible to produce hybrid grafts that can be used safely in many operations such as nasal reconstruction, rhinoplasty, auricle reconstruction, and repair of orbital floor fractures with the help of molds produced in more complex ways. CLINICAL RELEVANCE STATEMENT: Three-dimensionally-printed polycaprolactone molds can be used to shape diced cartilages in the areas of both aesthetic and reconstructive surgery.Publication Open Access Fabrication of electrospun juglans regia (juglone) loaded poly(lactic acid) scaffolds as a potential wound dressing material(2022-05-01) ALTAN, ERAY; ŞAHİN, ALİ; GÜNDÜZ, OĞUZHAN; ULAĞ, SONGÜL; ALTAN E., Karacelebi Y., Saatcioglu E., ULAĞ S., ŞAHİN A., AKSU M. B., Croitoru A., Codrea C. I., Ficai D., GÜNDÜZ O., et al.Juglone (5-hydroxy-1,4-naphthoquinone) (J) is a naphthoquinone structured allelochemical that is mostly found in the roots, leaves, nut-hulls, bark, and wood of walnut (Juglans regia). In this study, the biocompatibility, mechanical, thermal, chemical, morphological, and antimicrobial properties of the poly(lactic acid) (PLA) (w/v)/J (10, 20, 30 mg) electrospun scaffolds were investigated. Based on the results of the study, it was shown that juglone addition increased the antimicrobial properties of the scaffolds against the Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), compared to the neat PLA film after 24 h of contact time. According to the tensile test results, the addition of J made the scaffolds more flexible but decreased the mechanical strength. The cytotoxicity properties of the J-added scaffolds demonstrated a toxic behavior on the first day of incubation. However, with an increase in the J ratio, the fibroblast cell metabolic activity increased for all incubation periods.Publication Open Access Gentamicin and fluconazole loaded electrospun polymethylmethacrylate (PMMA) fibers as a novel platform for the treatment of corneal keratitis(2022-05-01) GÜNDÜZ, OĞUZHAN; ULAĞ, SONGÜL; GÜNDÜZ O., ULAĞ S.In this study, 10 mg fluconazole (10 FCZ) and 10 mg gentamicin (10 GEN) loaded with 40% polymethylmethacrylate (40% PMMA) fibers were fabricated by electrospinning and the performance of the formulations (40% PMMA, 40% PMMA/10 FCZ, 40% PMMA/10 GEN, and 40% PMMA/10 FCZ/10 GEN) was tested. 10 mg Gentamicin-loaded 40% PMMA fiber mat showed a more significant zone of inhibition against the S. aureus compared to the zone of 40% PMMA/10 GEN/10 FCZ fiber. The biocompatibility test using a human adipose-derived mesenchymal stem cell (MSCs) as a cell model proved that the amount of loaded and released GEN has no toxic effects on the MSCs.