Person: ŞEHİRLİ, ÜMİT SÜLEYMAN
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ŞEHİRLİ
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ÜMİT SÜLEYMAN
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Publication Open Access The effects of melatonin on the striatum(MARMARA UNIV, FAC MEDICINE, 2021-10-31) BORACI, HATİCE; Gergin, Sinem; Kirazli, Ozlem; Boraci, Hatice; Yildiz, Sercan Dogukan; Sehirli, Umit SuleymanObjective: Some of the neurological diseases cause morphologic changes in the striatal neurons. Medial forebrain bundle (MFB) lesion is a commonly used method to produce a Parkinsonian model rat. Melatonin is a hormone which exerts a neuroprotective effect on the neurons. The aim of this study is to investigate the effect of melatonin on the dendritic morphology of striatal medium spiny neurons (MSNs) in rats with MFB lesion. Materials and Methods: Twelve male Wistar albino rats were given saline injections into the MFB and divided into sedentary and treatment groups. The treatment group was administered a 10 mg/kg dose of melatonin intraperitoneally for 30 days. The lesion was confirmed histologically by Nissl staining. Golgi staining technique was applied to observe neuronal morphology. Neuronal structures were analysed from three-dimensional images by Neurolucida (MBF Bioscience) software. Results: The MFB lesion caused a reduction in the total dendritic length and in the number of dendritic endings. The melatonin enhanced the number of dendritic endings compared to the sedentary group. The melatonin led to an increase in the total spine density, spine densities of thin and mushroom types. Conclusion: Melatonin improved the dendritic degeneration due to MFB lesion.Publication Metadata only Wistar ve genetik absans epilepsili (GAERS) sıçanlarda dendrit morfolojisinin incelenmesi(2021-11-21) YAZI, SEVDENUR; ŞEHİRLİ, ÜMİT SÜLEYMAN; GÜLHAN, REZZAN; KİRAZLI, ÖZLEM; Yazı S., Şehirli Ü. S., Gülhan R., Onat F., Kirazlı Ö.Publication Metadata only Evaluation of dendrite morphology in wistar and genetic absence epileptic (GAERS) rats(2022-07-09) ŞEHİRLİ, ÜMİT SÜLEYMAN; KİRAZLI, ÖZLEM; GÜLHAN, REZZAN; YAZI S., ŞEHİRLİ Ü. S., GÜLHAN R., ONAT F., KİRAZLI Ö.Aims The aim of the study is to examine the morphological features of dendrites and dendritic spines of pyramidal neurons in somatosensory cortex and hippocampus of Wistar and GAERS (Genetic Absence Epilepsy Rat from Strasbourg) rats. Methods GAERS (n=5) and Wistar (n=5) rats were sacrificed by transcardial perfusion method. Brain tissues were stained using the FD Rapid GolgiStain Kit. Coronal sections of 200 µm thickness were obtained with cryostat. Pyramidal neurons in deep layers of the somatosensory cortex and CA1 region of the hippocampus were examined using light microscope and Neurolucida 360 software. Branching and length of apical dendrite and basal dendrites, and types and densities of dendritic spines were analyzed. Results Statistically the total number of dendrite nodes (p=0.0053, p=0,0047), the total number of dendrite segments (dendritic arborization) (p=0.0036, p=0,0036), the total number of dendrite terminations (p=0.0033, p=0,0029), the total dendrite length (µm) (p=0.0002, p=0,0007) and the dendritic spine density (1/µm) (p=0.0168, p=0,0120) of the somatosensory cortex and the hippocampus were significantly higher in GAERS rats, respectively. When dendritic spine types were evaluated separately, stubby type dendritic spines in the hippocampus were found to be significantly higher in GAERS rats compared to Wistar rats (p=0.0204). Conclusions It was concluded that intense synaptic activation seen in postsynaptic cells in the somatosensory cortex and hippocampus in GAERS causes changes in the dendrite morphology of pyramidal neurons. This study was supported by Marmara University Scientifical Research Projects Commission (TYL-2021-10244).Publication Open Access The failure on the effectiveness of formalin on cadaver disinfection and alternative methods(2023-01-01) AKSU, MEHMET BURAK; ÖZKAN, MAZHAR; ŞEHİRLİ, ÜMİT SÜLEYMAN; Yanilmaz O., Guncu M. M., AKSU M. B., ÖZKAN M., ŞEHİRLİ Ü. S.Objective: It was aimed to identify the contaminant and determine the alternative disinfectant detection in the microbial growth observed in various parts of the cadaver stored in the formalin tank in the dissection laboratory of Marmara University Anatomy Department. We also performed a literature review of this unusual pathogen. Materials and Methods: Swab samples were inoculated on agar mediums. After incubation, matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS) analysis was used to identify the isolate from the detected uniform colonies. Sample solution from the cadaver tank, freshly prepared 5% formalin and 0.55% ortho-phthalaldehyde were used to determine the disinfectant sensitivity of the isolate. Results: According to 16s rDNA sequence analysis, it was concluded as Skermanella aerolata with 99% similarity. In the disinfectant susceptibility test, it was observed that S. aerolata and control bacteria could grow in 5% formalin taken from the cadaver tank. No growth was detected in other disinfectants. Conclusion: To prevent cadaver contamination in anatomy laboratories, the quality control of the embalming solutions and indoor air filtration of the dissection rooms should be checked at regular intervals. Members of Skermanella genus have been identified as environmental organisms in several studies, however, recent researches reported this bacterium as a human pathogen.Publication Metadata only The effect of growth hormone on 6-OHDA inducedHEK-YFP-DAT cell line(2022-11-02) KİRAZLI, ÖZLEM; ŞEHİRLİ, ÜMİT SÜLEYMAN; ARMAN, AHMET; KİRAZLI Ö., Şenkal S., Aslan K., HAYAL T. B., DOĞAN A., ŞAHİN F., ŞEHİRLİ Ü. S., ARMAN A.Publication Open Access The effects of regular swimming exercise and melatonin on the neurons localized in the striatum of hemiparkinsonian rats(2022-10-01) KİRAZLI, ÖZLEM; BORACI, HATİCE; YILDIZ, SERCAN DOĞUKAN; YANANLI, HASAN RACİ; ŞEHİRLİ, ÜMİT SÜLEYMAN; Gergin S., KİRAZLI Ö., BORACI H., YILDIZ S. D. , YANANLI H. R. , ŞEHİRLİ Ü. S.Parkinson's disease is a progressive neurodegenerative movement disorder. We aimed to investigate the effects of regular swimming exercise and melatonin applied in the 6-Hydroxydopamine-induced Parkinson's disease rats by analysing dendritic spine of striatal neurons. Twenty-four male Wistar albino rats were used. 6-Hydroxydopamine unilaterally injected four (control, exercise, melatonin and exercise + melatonin) groups were included in the study. Tyrosine hydroxylase expression was detected by immunohistochemistry. Neurons and structures were identified from three-dimensional images by Neurolucida software. There was not any apparent difference for tyrosine hydroxylase positive neurons in the substantia nigra pars compacta and fibres in the striatum between the lesion sides of hemiparkinsonian groups. The treatment groups blocked the apomorphine-induced increase in rotations compared to the control group. In stepping test, the treatment groups prevented the loss of stepping in the contralateral side of hemiparkinsonian groups. The melatonin mostly had a positive effect on motor activity tests. In morphological analyses, the 6-Hydroxydopamine-induced lesion led to the reduction of the total dendritic length and number of branches. In the treatment groups, the reduction of the dendritic parameters was not observed. 6-Hydroxydopamine lesion led to a decrease in the total spine density, spine densities of thin and mushroom types. The exercise and melatonin treatments prevented the loss of spine density. The exercise treatment prevented the loss of spine density of mushroom type spines. The melatonin treatment blocked the loss of spine density of stubby type. In conclusion, these results provide evidence for effective additional protective therapeutic strategies for Parkinson\"s disease. In conclusion, results from the current study provide evidence for swimming exercise and melatonin as a promising candidate for effective additional protective strategies for PD.Publication Open Access The Effect of Canagliflozin on Hippocampal Dendrite Morphology in a Model of Alzheimer's Disease Induced by Intracerebroventricular Injection of Streptozotocin(2023-10-01) YAZI, SEVDENUR; YANANLI, HASAN RACİ; ŞEHİRLİ, ÜMİT SÜLEYMAN; KİRAZLI, ÖZLEM; Yazı S., Özen B., Buldu B., Yalçın E., Karaköse O., Çakmak O., Somunkıran S., Yananlı H. R., Şehirli Ü. S., Kirazlı Ö.Publication Metadata only Genetik absans epilepsili ve epileptik olmayan sıçanlarda nigrostriatal yolak dejenerasyonunun kalretinin immunoreaktivitesi üzerine etkisi(2022-11-10) KİRAZLI, ÖZLEM; GÜLÇEBİ İDRİZ OĞLU, MEDİNE; ŞEHİRLİ, ÜMİT SÜLEYMAN; GÜLHAN, REZZAN; KİRAZLI Ö., Turgan Aşık Z. N., Çulpan Y., GÜLÇEBİ İDRİZ OĞLU M., ŞEHİRLİ Ü. S., GÜLHAN R., ONAT F.Amaç: Kalsiyum bağlayıcı proteinler, nöronal işlev ve nörogenezde önemli rol oynamaktadır. Kalretinin, dikensiz GABAerjik internöronlarda eksprese edilmekte ve gelen dopaminerjik afferentlerle ve glutamaterjik kortikostriatal afferentlerle sinaptik bağlantı kurmaktadır. Yaşla birlikte azalabilen kalretinin, ileri yaşlardaki motor fonksiyonda oluşacak kayıplardan sorumlu tutulabilmektedir. Parkinson modeli sıçanlarda nigro -striatal yolaktaki dopaminerjik nöron kaybı sonrasında striatumda bulunan kalretinin pozitif internöronlarda azalma meydana geldiği gösterilmiştir. Bu azalışa glutamat reseptörlerindeki aşırı aktivite ve dopaminin eksikliğinin neden olduğu iddia edilmiştir. Kalretinin pozitif internöronların temporal epilepsiye karşı hassas olduğu gösterilmiştir. Epileptogenez sürecinde kimyasal lezyon ile nigro-striatal yolağın devre dışı bırakılmasının kalretinin sentezleyen nöronlar üzerine etkisi incelenmiştir. Gereç ve Yöntem: Striatum, Substantia Nigra pars compacta ve pars reticulata da bulunan kalretinin içeren nöronların tespiti için medial ön beyin demetine 6-OHDA uygulanan Wistar (n=5) GAERS (n=5) Wistar kontrol (n=5) ve GAERS kontrol (n=5) 40µm’lik sıçan beyin kesitlerine immohistokimya yöntemi uygulanmıştır. Nöronların sayım işlemleri floresans boyanmış kesitler üzerinden yapılmıştır. İstatistiksel değerlendirme için GraphPad Prism Tek yönlü ANOVA ve gruplar arasındaki karşılaştırma kullanılmıştır. Bulgular: Çalışmamızda (6 –OHDA) enjekte edilen Wistar ve GAERS sıçanlarda kontrol gruplarına göre kalretinin pozitif hücre sayısında azalma gözlenmiştir. Bu azalma Striatum için istatistiksel olarak anlamlı bulunmazken, Substantia Nigra pars compacta (p<0,05) ve pars reticulata (p<0,05) için anlamlıdır. Sonuç: Literatürde (6 –OHDA) enjekte edilen sıçanlarda nigrostriatal yolakta sağlam kalan dopaminerjik nöronların kalretinin eksprese ettiği gözlemlenmiştir. Bu sonuçlar sonrasında, kalretininin nöroprotektif etkisinden dolayı dopaminerjik nöronları koruyabildiği ileri sürülebilir.Publication Metadata only Evaluation of dendrite morphology in wistar andgenetic absence epileptic (GAERS) rats(2022-08-30) ŞEHİRLİ, ÜMİT SÜLEYMAN; KİRAZLI, ÖZLEM; GÜLHAN, REZZAN; YAZI S., ŞEHİRLİ Ü. S., GÜLHAN R., ONAT F., KİRAZLI Ö.Publication Metadata only Intraserebroventriküler streptozosin enjeksiyonu ile oluşturulmuş Alzheimer modelinde kanagliflozin'in Hipokampus dendrit morfolojisi üzerine etkisi(2023-06-08) YAZI, SEVDENUR; YANANLI, HASAN RACİ; ŞEHİRLİ, ÜMİT SÜLEYMAN; KİRAZLI, ÖZLEM; Yazı S., Özen B., Buldu B., Yalçın E., Karaköse O., Çakmak O., Somunkıran S., Yananlı H. R., Şehirli Ü. S., Kirazlı Ö.