Publication: Evaluation of dendrite morphology in wistar and genetic absence epileptic (GAERS) rats
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Date
2022-07-09
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Abstract
Aims The aim of the study is to examine the morphological features of dendrites and dendritic spines of pyramidal neurons
in somatosensory cortex and hippocampus of Wistar and GAERS (Genetic Absence Epilepsy Rat from Strasbourg)
rats. Methods GAERS (n=5) and Wistar (n=5) rats were sacrificed by transcardial perfusion method. Brain tissues were
stained using the FD Rapid GolgiStain Kit. Coronal sections of 200 µm thickness were obtained with cryostat. Pyramidal
neurons in deep layers of the somatosensory cortex and CA1 region of the hippocampus were examined using light
microscope and Neurolucida 360 software. Branching and length of apical dendrite and basal dendrites, and types and
densities of dendritic spines were analyzed. Results Statistically the total number of dendrite nodes (p=0.0053, p=0,0047),
the total number of dendrite segments (dendritic arborization) (p=0.0036, p=0,0036), the total number of dendrite terminations
(p=0.0033, p=0,0029), the total dendrite length (µm) (p=0.0002, p=0,0007) and the dendritic spine density (1/µm) (p=0.0168,
p=0,0120) of the somatosensory cortex and the hippocampus were significantly higher in GAERS rats, respectively. When
dendritic spine types were evaluated separately, stubby type dendritic spines in the hippocampus were found to be
significantly higher in GAERS rats compared to Wistar rats (p=0.0204). Conclusions It was concluded that intense synaptic
activation seen in postsynaptic cells in the somatosensory cortex and hippocampus in GAERS causes changes in the
dendrite morphology of pyramidal neurons. This study was supported by Marmara University Scientifical Research Projects
Commission (TYL-2021-10244).
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Keywords
Absence epilepsy, GAERS, dendrite, dendritic spine
Citation
YAZI S., ŞEHİRLİ Ü. S., GÜLHAN R., ONAT F., KİRAZLI Ö., \"Evaluation of Dendrite Morphology in Wistar and Genetic Absence Epileptic (GAERS) Rats
\", Federation of European Neuroscience Congress, Paris, Fransa, 09 Temmuz 2022