Person: BİTİŞ, LEYLA
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BİTİŞ
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LEYLA
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Publication Metadata only Chemical composition of endemic Stachys subnuda Montbret & Aucher ex Benth. essential oil and its anti-inflammatory and antioxidant activities(TAYLOR & FRANCIS INC, 2019) DOĞAN, AHMET; Sen, Ali; Kurkcuoglu, Mine; Bitis, Leyla; Dogan, Ahmet; Baser, Kemal Husnu CanThe essential oil of aerial parts of Stachys subnuda Montbret & Aucher ex Benth (S. subnuda) was analyzed by gas chromatography (GC) and GC/mass spectrometry (MS). Anti-inflammatory activity was evaluated by lipoxygenase inhibition assay. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) assays. Sixty-two constituents, comprising 97.1% of the total essential oil composition, were identified. Phytol (25.2%), beta-caryophyllene (11.2%), germacrene D (6.9%), and hexadecanoic acid (6.9%) were the major compounds of aerial part essential oils of S. subnuda. The essential oil showed significant radical scavenging activity with 50% inhibition (IC50) values of 0.124 and 0.204 mg/mL for ABTS and DPPH radicals, respectively. The essential oil exhibited moderate anti-inflammatory activity with an IC50 value of 0.419 mg/mL. The results indicated that S. subnuda essential oil had good anti-inflammatory and antioxidant activity. Also, this is first study on chemical composition, anti-inflammatory and antioxidant activity of S. subnuda essential oil.Publication Open Access In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna ( K.Koch) Wagenitz(MARMARA UNIV, 2022) ŞEN, ALİ; Yildirim, Aybeniz; Sen, Ali; Goger, Fatih; Ozakpinar, Ozlem Bingol; Bitis, LeylaPublication Open Access Two Acylated Isoscutellarein Glucosides with Anti-Inflammatory and Antioxidant Activities Isolated from Endemic Stachys Subnuda Montbret & Aucher ex Benth(SLOVENSKO KEMIJSKO DRUSTVO, 2019-12-18) DOĞAN, AHMET; Sen, Ali; Goger, Fatih; Dogan, Ahmet; Bitis, LeylaIn this study, we report anti-inflammatory and antioxidant activities of two acylated isoscutellarein glucosides isolated from ethyl acetate extract of Stachys subnuda aerial part. 4'-O-methylisoscutellarein-7-O-2 ''-O-(6 ''' acetyl-beta-D-allopyranosyl)-beta-D-glucopyranoside (SS1) and isoscutellarein-7-O-2 ''-O-(6 '''-O-acetyl-beta-D allopyranosyl)-beta-D -glucopyranoside (SS2) were isolated as major compounds from ethyl acetate extract (SSEA). Also, 2 hydroxycinnamic acid derivatives, and 5 isoscutellarein glucoside derivatives in the SSEA were identified using LC-MS/MS. SS1 with IC50 values of 2.35 and 1.98 mu g/mL and SS2 with IC50 values 13.94 and 12.76 mu g/mL showed fairly strong antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) radicals, respectively. SS1 and SS2 inhibited 5-lipoxygenase (5-LOX) activity with IC(50 )values of 47.23 and 41.60 mu g/mL, respectively. The results demonstrated that SS1 and SS2 have significant anti-inflammatory and antioxidant potential. Acylated flavonoid glycosides have been first reported for Stachys subnuda. Also, the activities of SS1 and SS2 have been investigated for the first time in this study.Publication Open Access Flavonoids and biological activities of various extracts from Rosa sempervirens leaves(TAYLOR & FRANCIS LTD, 2017-03-04) ŞEN, ALİ; Bitis, Leyla; Sen, Ali; Ozsoy, Nurten; Birteksoz-Tan, Seher; Kultur, Sukran; Melikoglu, GulayRosa sempervirens L. (Rosaceae) growing wildly in Turkey is used in folk medicine for various indications. Here, we report the isolation of four flavonoids and determination of the total phenolic and flavonoid contents and the antimicrobial and antioxidant activities of various extracts from R. sempervirens leaves. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS). The total phenolic and flavonoid contents were determined using the Folin-Ciocalteu and AlCl3 methods, respectively. The antimicrobial activity was evaluated by the broth microdilution method against seven microbial species. The ethyl acetate extract (E) had significant antioxidant activity with half maximal inhibitory concentration (IC50) values of 3.96 in the DPPH and 2.92 mg/mL in the ABTS assay. The total phenolic (203.8 mg gallic acid equivalents/g extract) and total flavonoid (95.81 mg catechin equivalents/g extract) contents of the E extract were significantly higher as compared to other extracts. The E extract exhibited strong antimicrobial activity against Candida albicans with a minimum inhibitory concentration of 39 mg/mL. Quercetin 3-xyloside, quercitrin and hyperoside were isolated from the E extract and quercetin, from the chloroform extract, and quercetin and hyperoside were identified for the first time in this species. Quercitrin was found to be a major compound in the E extract. Antimicrobial activity of R. sempervirens was also reported for the first time. These results indicate that the E extract has significant antioxidant and antimicrobial activity, probably due to flavonoids as well as other phenolic compounds in the E extract, acting individually or in combination.Publication Metadata only Chemical composition, antiradical, and enzyme inhibitory potential of essential oil obtained from aerial part of Centaurea pterocaula Trautv(TAYLOR & FRANCIS INC, 2021) ŞEN, ALİ; Sen, Ali; Kurkcuoglu, Mine; Yildirim, Aybeniz; Senkardes, Ismail; Bitis, Leyla; Baser, Kemal Husnu CanThe chemical composition, free radical scavenging, alpha-amylase inhibitory, and 5-lipoxygenase inhibitory activity of the essential oil obtained from the aerial parts of Centaurea pterocaula Trautv. were examined. Twenty-nine compounds corresponding to 93.2% of the oil were identified in the oil of C. pterocaula. Hexadecanoic acid (13.9%), caryophyllene oxide (11.5%), spathulenol (11.4%), (E)-beta-damascenone (5.0%), hexahydrofarnesyl acetone (5.0%), and tetradecanoic acid (4.9%) were the main compounds of the oil. The oil exhibited good anti-inflammatory activity, inhibiting 5-lipoxygenase with an IC50 value of 86.22 mu g/mL. The oil was found to be active against DPPH radical and alpha-amylase with IC50 values of 83.05 and 79.66 mu g/mL respectively, while it showed moderate activity against ABTS radicals with IC50 value of 360.9 mu g/mL. To the best of our knowledge, this is first report on the chemical composition of C. pterocaula essential oil from Turkey; as well as on the biological activity of its essential oil.