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AYDIN OMAY, BANU

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AYDIN OMAY

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Now showing 1 - 4 of 4
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    Flavonoid ile Kombine Edilmiş Kemoterapi İlaçlarının Kolorektal Kanser Hücrelerine Etkisi
    (2022-12-09) AYDIN OMAY, BANU; ERZİK, CAN; ARĞA, KAZIM YALÇIN; CABADAK, HÜLYA; Kanlı Z., Aydın Omay B., Erzik C., Arğa K. Y. , Cabadak H.
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    Potential antiproliferative and apoptotic effects of pilocarpine combined with TNF alpha in chronic myeloid leukemia cells
    (2023-01-01) CABADAK, HÜLYA; AYDIN OMAY, BANU; Kanlı Z., CABADAK H., AYDIN OMAY B.
    © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.Pilocarpine is a selective M1/M3 agonist of muscarinic acetylcholine receptor subtypes. Muscarinic acetylcholine receptors are G protein-coupled receptors. These receptors are different drug targets. The aim of the present work was to investigate the effect of pilocarpine on the expression of M3 muscarinic acetylcholine receptor, the AChE activity, IL-8 release response, and proliferation in K562 cells, via muscarinic receptor activation. Human chronic myeloid leukemic cell cultures were incubated with drugs. Proliferation assays were performed by BrdU assay. Expression of M3 muscarinic acetylcholine receptor and apoptosis proteins such as bcl, bax, cyt C, and caspases was assessed with the semiquantitative Western blotting method. Pilocarpine inhibits chronic myeloid cell proliferation and M3 muscarinic acetylcholine receptor protein expression. Pilocarpine increases caspase-8 and -9 expression levels, upregulating the proapoptotic protein Bax and downregulating the expression levels of the antiapoptotic protein Bcl-2. The apoptotic activity of pilocarpine is associated with an increase in AChE activity. M3 muscarinic acetylcholine receptors can activate multiple signal transduction systems and mediate inhibitory effects on chronic myeloid K562 cell proliferation depending on the presence of 1% FBS conditions. This apoptotic effect of pilocarpine may be due to the concentration of pilocarpine and the increase in AChE level. Our results suggest that inhibition of cell proliferation by inducing apoptosis of pilocarpine in K562 cells may be one of the targets. M3 selective agonist may have therapeutic potential in chronic myeloid leukemia. Graphical Abstract: [Figure not available: see fulltext.].
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    Effects of melatonin supplementation and different exercise models on cognitive function in long-term exposure to constant light in rats
    (2023-01-01) YILDIRIM, HATİCE SELİN; AYDIN OMAY, BANU; KASIMAY ÇAKIR, ÖZGÜR; Erol G., Dincer B. E., Calik H. N., Oksuz H., YILDIRIM H. S., Sener D., AYDIN OMAY B., KASIMAY Ö.
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    Hepatosellüler karsinomada glut1 inhibitörü bay-876’nın hepg2 hücrelerinde ve steatoz hücre modelinde glukoz alımı ve hücre çoğalmasına etkisi
    (2022-12-09) AYDIN OMAY, BANU; CABADAK, HÜLYA; Tanriverdi Bademci A. M., Aydın Omay B., Cabadak H.
    Hepatocellular carcinoma is one of the deadly cancers with a significant incidence worldwide. In cancer cells, aerobic glycolysis increases with the Warburg effect. Glucose transport across the cell membrane becomes important at this stage. It has been found that there is an increase in GLUT1 expression in various cancers. BAY-876 is a new generation GLUT1 inhibitor. The affinity of BAY-876 for GLUT1 was found to be approximately 100 times higher than for other glucose transporters. HepG2 cells will be used in molecular analyzes to investigate the role of this novel GLUT1 inhibitor in GLUT1-mediated cell proliferation and glucose uptake into the cell.The effect of BAY-876 on glucose uptake was investigated in the case of bioenergetic stress by applying metformin, an insulin-sensitizing agent with strong antihyperglycemic properties, and in hypoxic environment by applying CoCl2 treatment. A steatosis cell model was created using oleic acid and palmitic acid. We aimed to investigate the effects of BAY-876 on cell proliferation and glucose uptake in HepG2 cell line and HepG2 steatosis cell model. Drugs were added to the culture medium. its effect on cell proliferation was investigated with the BrDU method. Cell proliferation, glucose uptake and colony formation were investigated in HepG2 cell line and HepG2 steatosis cell model in the presence and absence of BAY-876. BAY-876 inhibits cell proliferation and reduces glucose uptake in HepG2 cells and steatosis cell model. BAY-876 was also found to reduce glucose uptake in normal and steatosis cell models under bioenergetic and hypoxic stress conditions. It is predicted that our study will contribute to the literature, provide a different perspective to anticancer drug research studies of GLUT1 inhibitor and contribute to the elucidation of the signal transmission mechanism of hepatocellular carcinoma.