Publication: Hepatosellüler karsinomada glut1 inhibitörü bay-876’nın hepg2 hücrelerinde ve steatoz hücre modelinde glukoz alımı ve hücre çoğalmasına etkisi
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Date
2022-12-09
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Abstract
Hepatocellular carcinoma is one of the deadly cancers with a significant incidence worldwide. In cancer
cells, aerobic glycolysis increases with the Warburg effect. Glucose transport across the cell membrane
becomes important at this stage. It has been found that there is an increase in GLUT1 expression in
various cancers. BAY-876 is a new generation GLUT1 inhibitor. The affinity of BAY-876 for GLUT1
was found to be approximately 100 times higher than for other glucose transporters. HepG2 cells will
be used in molecular analyzes to investigate the role of this novel GLUT1 inhibitor in GLUT1-mediated
cell proliferation and glucose uptake into the cell.The effect of BAY-876 on glucose uptake was
investigated in the case of bioenergetic stress by applying metformin, an insulin-sensitizing agent with
strong antihyperglycemic properties, and in hypoxic environment by applying CoCl2 treatment. A
steatosis cell model was created using oleic acid and palmitic acid. We aimed to investigate the effects
of BAY-876 on cell proliferation and glucose uptake in HepG2 cell line and HepG2 steatosis cell model.
Drugs were added to the culture medium. its effect on cell proliferation was investigated with the BrDU
method. Cell proliferation, glucose uptake and colony formation were investigated in HepG2 cell line
and HepG2 steatosis cell model in the presence and absence of BAY-876. BAY-876 inhibits cell
proliferation and reduces glucose uptake in HepG2 cells and steatosis cell model. BAY-876 was also
found to reduce glucose uptake in normal and steatosis cell models under bioenergetic and hypoxic
stress conditions. It is predicted that our study will contribute to the literature, provide a different
perspective to anticancer drug research studies of GLUT1 inhibitor and contribute to the elucidation of
the signal transmission mechanism of hepatocellular carcinoma.
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Tıp, Sağlık Bilimleri, Temel Tıp Bilimleri, Biyofizik, Medicine, Health Sciences, Fundamental Medical Sciences, Biophysics, Klinik Tıp (MED), Yaşam Bilimleri (LIFE), Klinik Tıp, Biyoloji ve Biyokimya, TIP, GENEL & DAHİLİ, BİYOFİZİK, Clinical Medicine (MED), Life Sciences (LIFE), CLINICAL MEDICINE, BIOLOGY & BIOCHEMISTRY, MEDICINE, GENERAL & INTERNAL, BIOPHYSICS, Genel Sağlık Meslekleri, Patofizyoloji, Temel Bilgi ve Beceriler, Değerlendirme ve Teşhis, Dahiliye, Aile Sağlığı, Tıp (çeşitli), Genel Tıp, Yaşam Bilimleri, General Health Professions, Pathophysiology, Fundamentals and Skills, Assessment and Diagnosis, Internal Medicine, Family Practice, Medicine (miscellaneous), General Medicine, Life Sciences, Hepatocellular carcinoma, BAY-876, steatosis, metformin
Citation
Tanriverdi Bademci A. M., Aydın Omay B., Cabadak H., \"HEPATOSELLÜLER KARSİNOMADA GLUT1 İNHİBİTÖRÜ BAY-876’NIN HEPG2 HÜCRELERİNDE VE STEATOZ HÜCRE MODELİNDE GLUKOZ ALIMI VE HÜCRE ÇOĞALMASINA ETKİSİ\", 9. ULUSLARARASI MARMARA FEN BİLİMLERİ KONGRESİ, Kocaeli, Türkiye, 09 Aralık 2022