Person: ALTURFAN, EBRU IŞIK
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
ALTURFAN
First Name
EBRU IŞIK
Name
68 results
Search Results
Now showing 1 - 10 of 68
Publication Metadata only Clinical and Biochemical Evaluation of Lozenges Containing Lactobacillus reuteri as an Adjunct to Non-Surgical Periodontal Therapy in Chronic Periodontitis(WILEY, 2015) ALTURFAN, EBRU IŞIK; Ince, Gizem; Gursoy, Hare; Ipci, Sebnem Dirikan; Cakar, Gokser; Emekli-Alturfan, Ebru; Yilmaz, SelcukBackground: This study evaluates the effects on clinical and biochemical parameters of Lactobacillus reuteri-containing probiotic supplementation adjunctive to initial periodontal therapy in patients with chronic periodontitis (CP). Methods: Thirty patients with CP were included and divided into two groups. Every patient had, in each quadrant, >= 2 teeth each with approximal sites with a probing depth (PD) of 5 to 7 mm and gingival index (GI) of >= 2. The test group received scaling and root planing (SRP) and probiotic-containing lozenges. The control group received SRP and placebo lozenges. Plaque index (PI), GI, bleeding on probing (BOP), PD, and attachment gain were measured. Gingival crevicular fluid (GCF) was sampled for the analysis of matrix metalloproteinase (MMP)-8 and tissue inhibitor of metalloproteinase (TIMP)-1 by enzyme-linked immunosorbent assay. All evaluations were performed at baseline and on days 21, 90, 180, and 360. Results: Differences in intergroup comparisons of PI, GI, BOP, and PD were found to be significant (P < 0.05) in favor of the test group at all time points. Decreased GCF MMP-8 levels and increased TIMP-1 levels were found to be significant up to day 180 (P < 0.05). Mean values of attachment gain were significantly higher in the test group compared with the control group on days 90, 180, and 360. Conclusions: Lozenges containing L. reuteri may be a useful supplement in moderately deep pockets of patients with CP. Low MMP-8 and high TIMP-1 levels may indicate the role of the lozenges in reduction of inflammation-associated markers up to day 180.Publication Metadata only From epidemiology to treatment: Aspirin's prevention of brain and breast-cancer and cardioprotection may associate with its metabolite gentisic acid(ELSEVIER IRELAND LTD, 2018) ALTURFAN, EBRU IŞIK; Altinoz, Meric A.; Elmaci, Ilhan; Cengiz, Salih; Emekli-Alturfan, Ebru; Ozpinar, AyselBackground: Epidemiological studies indicate that aspirin consumption reduces the risk of tumors, which is especially relevant for colonic adenoma and carcinoma. Similar observations were made for glial brain tumors and breast cancers, yet the results are inconsistent. Gentisic acid (GA) is a minor catabolite of aspirin; yet humans carrying CYP2C9-variants incapable to catabolize aspirin to GA do not benefit from aspirin in prevention against colonic adenoma. GA blocks binding of Fibroblastic Growth Factor to its receptor and its sulphonate metabolite dobesilic acid blocks growth of C6 glioblastoma in vivo. GA is also an endogenously produced siderophore in mammalians for the transport of iron, a trace element which stimulates tumor growth and enhances anthracycline cardiotoxicity. Materials and Methods: In this study, we assessed whether GA exerts direct antitumor activity on C6 glioma cells in vitro (cytotoxicity, colony growth, 3H-thyrnidine labeling analysis of DNA synthesis); and whether it can modify growth of Ehrlich breast ascites carcinoma (EAC) and solid tumors (EST) in vivo. GA and antitumoral trace element selenium block 12-lipoxygenase activity and aspirin's paradoxical inflammatory effects are seen in selenium-deficient humans; thus, we also investigated antitumor interactions between GA and sodium selenite. Lastly, we evaluated whether GA could protect against doxorubicin cardiotoxicity due to its function to chelate iron. Results: Clinically achievable doses of GA blocked growth, colony formation and DNA synthesis of C6 glioma in vitro with high significance. GA enhanced the survival of EAC-bearing mice at a dosage of 0.4 mg/mice/day, in which 33% of the treated animals survived more than 3-weeks, when all untreated mice succumbed to their tumors. Selenium decreased EST volumes initially, yet increased tumor volumes at later stages in surviving mice. GA alone reduced solid tumor growth and did not modify selenite antineoplasticity initially, but blocked the late tumor-stimulating effects of selenite. Lastly, doxorubicin-induced cardiac myofibrillary and endothelial damage and hyalinization necrosis were attenuated with GA treatment. Conclusions: GA highly merits to be studied in further animal models as an anticancer and chemoprotective drug.Publication Open Access Morphine ameliorates pentylenetetrazole-induced locomotor pattern in zebrafish embryos; mechanism involving regulation of opioid receptors, suppression of oxidative stress, and inflammation in epileptogenesis(2023-01-01) ALTURFAN, EBRU IŞIK; MEGA TİBER, PINAR; Üstündağ F. D., Ünal İ., Üstündağ Ü. V., Cansız D., Beler M., Alturfan A. A., Tiber P., Emekli-Alturfan E. I.Zebrafish (Danio rerio) is becoming an increasingly important model in epilepsy research. Pentylenetetrazole (PTZ) is a convulsant agent that induces epileptic seizure-like state in zebrafish and zebrafish embryos and is most commonly used in antiepileptic drug discovery research to evaluate seizure mechanisms. Classical antiepileptic drugs, such as valproic acid (VPA) reduce PTZ-induced epileptiform activities. Opioid system has been suggested to play a role in epileptogenesis. The aim of our study is to determine the effects of morphine in PTZ-induced epilepsy model in zebrafish embryos by evaluating locomotor activity and parameters related to oxidant-antioxidant status, inflammation, and cholinergic system as well as markers of neuronal activity c-fos, bdnf, and opioid receptors. Zebrafish embryos at 72 hpf were exposed to PTZ (20 mM), VPA (1 mM), and Morphine (MOR) (100 mu M). MOR and VPA pretreated groups were treated with either MOR (MOR + PTZ) or VPA (VPA + PTZ) for 20 min before PTZ expoure. Locomotor activity was quantified as total distance moved (mm), average speed (mm/sec) and exploration rate (%) and analyzed using ToxTrac tracking programme. Oxidant-antioxidant system parameters, acetylcholinesterase activity, and sialic acid leves were evaluated using spectrophotometric methods. The expression of c-fos, bdnf, oprm1, and oprd1 were evaluated by RT-PCR. MOR pretreatment ameliorated PTZ-induced locomotor pattern as evidenced by improved average speed, exploration rate and distance traveled. We report the restoration of inflammatory and oxidant-antioxidant system parameters, c-fos, bdnf, and opioid receptor oprm1 as the possible mechanisms involved in the ameliorative effect of MOR against PTZ-induced epileptogenic process in zebrafish embryos.Publication Metadata only Neuroprotective effects of mitoquinone and oleandrin on Parkinson's disease model in zebrafish(TAYLOR & FRANCIS LTD, 2020) ALTURFAN, EBRU IŞIK; Unal, Ismail; Caliskan-Ak, Esin; Ustundag, Unsal V.; Ates, Perihan S.; Alturfan, Ahmet A.; Altinoz, Meric A.; Elmaci, Ilhan; Emekli-Alturfan, EbruAim: The aim of this study is to investigate the possible protective effects of mitoquinone and oleandrin on rotenone induced Parkinson's disease in zebrafish. Materials and methods: Adult zebrafish were exposed to rotenone and mitoquinone for 30 days. Biochemical parameters were determined by spectrophotometric method and Parkinson's disease-related gene expressions were determined by reverse transcription polymerase chain reaction method. Measurement of neurotransmitters was performed by liquid chromatography tandem-mass spectrometry instrument. The accumulation of synuclein was demonstrated by immunohistochemical staining. In vitro thiazolyl blue tetrazolium bromide method was applied to determine the mitochondrial function of synaptosomal brain fractions using rotenone as a neurotoxic agent and mitoquinone and oleandrin as neuroprotective agents. Results: Mitoquinone improved the oxidant-antioxidant balance and neurotransmitter levels that were disrupted by rotenone. Mitoquinone also ameliorated the expressions of Parkinson's disease-related gene expressions that were disrupted by rotenone. According to thiazolyl blue tetrazolium bromide assay results, mitoquinone and oleandrin increased mitochondrial function which was decreased due to rotenone exposure. Conclusion: Based on the results of our study, positive effects of mitoquinone were observed in Parkinson's disease model induced by rotenone in zebrafish.Publication Metadata only Zebrafish; an emerging model organism for studying toxicity and biocompatibility of dental materials(C M B ASSOC, 2020) ALTURFAN, EBRU IŞIK; Karaman, Gozde Ece; Emekli-Alturfan, Ebru; Akyuz, SerapZebrafish (danio rerio) is a small, tropical freshwater teleost fish that belongs to the Cyprinidae family and lives in natural waters and rice fields in South Asia, North India, and Pakistan. Zebrafish has become a popular vertebrate model organism for biomedical research due to its numerous advantages such as their small size, short life cycle, accessibility in large numbers and inexpensive maintenance. In addition, fertilization happens externally in zebrafish and allows zebrafish to be manipulated directly. As another important advantage, the embryos are transparent thus the stages of development can be easily identified. Zebrafish can have multiple co-orthologs for human genes. In the 1930s, the zebrafish was first used as a model for developmental and embryological studies and in 1981, was introduced as a genetic model by Streisinger by force of developed genetic techniques in zebrafish such as cloning, mutagenesis and transgenesis. In the 1990s, various genetic manipulations were introduced. These improvements have contributed to the popularity of zebrafish. After that zebrafish was used in various research areas including genetics, biomedicine, neurobiology, toxicology, pharmacology as well as in human disease models. Zebrafish is also becoming a popular model organism in dental research. It is preferred in dental material toxicity studies and in research related to the genetic and molecular factors in tooth formation and craniofacial development. This review provides information on the use of zebrafish in dental research, focusing on tooth formation and dentition (pharyngeal dentition) of zebrafish and the dental research performed using zebrafish.Publication Metadata only The effects of tacrolimus on the activity and expression of tissue factor in the rat ovary with ischemia-reperfusion induced injury(INST ANIMAL REPRODUCTION FOOD RESEARCH, 2015) ALTURFAN, EBRU IŞIK; Ustundag, Unsal Veli; Sahin, Sadik; Ak, Koray; Keskin, Ilknur; Emekli-Alturfan, EbruIn the present study, the effects of tacrolimus on the activity and expression of tissue factor (TF) were investigated in the ovarian ischemia-reperfusion induced injury in rats. Twenty-eight female rats (8-12 weeks, 300-350 g) were divided into four groups: control, ischemia-reperfusion (IR), tacrolimus treated before ischemia (TBI), and tacrolimus treated before reperfusion (TBR) groups (n = 7/per group). TF activity was measured using Quick's method, whereas TF expression was examined immunohistochemically. TF activity was significantly higher in all treated groups compared with the control group. Strong ovarian TF expression was demonstrated in the IR and TBR groups. Moreover, tacrolimus decreased TF activity in the TBI group compared with the IR group. The decreased activity of TF in the ovarian IR model may prevent IR-related inflammation during transplant procedure. (C) 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Sp. z.o.o. All rights reserved.Publication Metadata only Kronik periodontitisli ve sağlıklı kişilerin tükürük örneklerinin bazı biyokimyasal parametreler ve sitolojik incelemeler açısından karşılaştırılmasında saklama süresinin belirlenmesi(2009-06-20) ALTURFAN, EBRU IŞIK; YARAT, AYŞEN; AK, ESİN; ALTURFAN E. I., YARAT A., AK E., PİŞİRİCİLER R., KURU B., NOYAN Ü.Publication Metadata only Oxidative stress and apoptosis in electromagnetic waves exposed Zebrafish embryos and protective effects of conductive nonwoven fabric(C M B ASSOC, 2020) ALTURFAN, EBRU IŞIK; Ustundag, Unsal Veli; Ozen, Mustafa Sabri; Unal, Ismail; Ates, Perihan Seda; Alturfan, Ahmet Ata; Akalin, Mehmet; Sancak, Erhan; Emekli-Alturfan, EbruThe amount of technological products including television, radio transmitters, and mobile phone that have entered our daily life has increased in recent years. But these devices may cause adverse effects on human health. Electromagnetic shielding fabrics may limit and inhibit electromagnetic waves. Aim of our study was to evaluate electromagnetic wave blocking performance of nonwoven textile surfaces on zebratish embryos that were exposed to electromagnetic waves at specific frequencies. Oxidant-antioxidant system parameters were evaluated spectrophotometrically. The expressions of tp53 and casp3a were evaluated by RT-PCR. Results showed that electromagnetic shielding fabrics produced as conductive nonwoven textile surfaces improved oxidant-antioxidant status and 103 expression that were impaired in electromagnetic waves exposed zebrafish embryos. Also, electromagnetic shielding fabrics decreased casp3a expression responsible for the execution phase of apoptosis that increased in electromagnetic waves exposed zebrafish embryos.Publication Metadata only Zebra balıklarında dopaminerjik nöron hasarı(Türkiye Klinikleri Yayınevi, 2023-08-01) ALTURFAN, EBRU IŞIK; Alturfan E. I.Zebra balığı (Danio rerio), nörogelişimsel ve nörodejeneratif hastalıklarla ilgili araştırmalarda kullanılan omurgalı bir model organizmadır. Zebra balıklarında insanlardaki substantia nigraya benzer dopaminerjik nöron popülasyonlarının tanımlanması zebra balığının Parkinson Hastalığı fenotipinin indüklenmesi ve terapötik ilaçların taranması için umut verici bir model olabileceğini göstermiştir. Zebra balığında dopaminerjik sistem döllenmeden 96 saat sonra tamamen gelişmiştir ve tirozin hidroksilaz immünopozitif nöronları, dopaminerjik nöronlar olarak kabul edilir. Bu derlemede, zebra balığında dopaminerjik sistem ve gelişimi ile dopaminerjik nöron hasarı oluşturulmak için uygulanan kimyasal ajanlardan bahsedilmiştir. Bu ajanlardan lipofilik yapısından dolayı kan-beyin bariyerini kolayca geçebilen 1-Metil-4-Fenil-1,2,3,6-Tetrahidropiridin, zebra balıklarında dopaminerjik nöronlarının kaybını indükleyerek Parkinson hastalığı modeli oluşturmak için kullanılan bir nörotoksindir. Birer pestisit olan parakuat ve rotenon maruziyeti Parkinson hastalığı gelişimi ile ilişkilidir. Katekolamin yapısına olan benzerliği ile dopamin taşıyıcılarına afinitesi olan 6-hidroksidopamin oksidatif stres ve inflamasyonu indükleyerek dopaminerjik nöronlarda hasara neden olur. Bu ajanlar zebra balıklarında ve larvalarında dopaminerjik nöronlarda hasara neden olarak nörotoksik etki göstermiştir.Publication Metadata only The Relation between Plasma Tissue Factor and Oxidized LDL Levels in Acute Coronary Syndromes(KARGER, 2007) ALTURFAN, EBRU IŞIK; Emekli-Alturfan, Ebru; Basar, Isik; Alturfan, A. Ata; Ayan, Faruk; Koldas, Lale; Balci, Huriye; Emekli, NesrinAim: Tissue factor (TF) is a low-molecular-weight glycoprotein responsible for the initiation of the coagulation cascade. The relation between oxidized low-density lipoprotein (OxLDL), that has been shown to be involved in atherogenesis, and TF has not been evaluated before in circulating plasma. The aim of this study was to determine plasma levels of TF and Ox-LDL in acute coronary syndrome (ACS) and stable coronary artery disease (SCAD). Methods: The study group consisted of 41 patients with ACS and 26 patients with SCAD. Among the ACS patients, 12 were diagnosed with unstable angina pectoris (UAP) and 29 were diagnosed with acute myocardial infarction (AMI). The control group consisted of 30 healthy volunteers. TF and Ox-LDL levels were evaluated by ELISA kits. Results: Ox-LDL levels were significantly higher in UAP and AMI patients compared with the control (p < 0.001) and SCAD (p < 0.01 and p < 0.001, respectively) groups. TF levels were significantly higher in the UAP, AMI and SCAD groups compared with the control group (p < 0.001, p < 0.001 and p < 0.01, respectively). In the AMI group a significant increase was observed in TF levels when compared with the SCAD group (p < 0.01). Plasma Ox-LDL levels were significantly and positively correlated with TF levels in the UAP and AMI groups (p < 0.05, r = 702, and p < 0.0001, r = 0.679, respectively). Conclusion: The potential link between OxLDL and TF in circulating blood in ACS may strengthen the evidence supporting a relationship between oxidant stress, lipids and thrombosis and consequently may contribute to understanding the mechanism through which Ox-LDL and TF may mediate the pathogenesis of CAD. Copyright (C) 2010 S. Karger AG, Basel