Person: ŞAHİN, ALİ
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Publication Open Access Biocompatible polyvinyl alcohol nanofibers loaded with amoxicillin and salicylic acid to prevent wound infections.(2023-08-21) ŞAHİN, ALİ; Aydin A., Ulag S., Sahin A., Aksu B., Gunduz O., Ustundag C. B., Marinas I. C., Georgescu M., Chifiriuc M. C.Diabetic wounds are one of the most challenging clinical conditions in diabetes, necessitating the development of new treatments to foster healing and prevent microbial contamination. In this study, polyvinyl alcohol (PVA) was used as a matrix polymer, and amoxicillin (AMX) and salicylic acid (SA) were selected as bioactive compounds with antimicrobial (with AMX) and anti-inflammatory action (with SA) to obtain innovative drug-loaded electrospun nanofiber patches for the management of diabetic wounds. Scanning electron microscopy (SEM) images revealed the uniform and beadless structure of the nanofiber patches. Mechanical tests indicated that AMX minimally increased the tensile strength, while SA significantly reduced it. The patches demonstrated effective antibacterial activity against both grampositive (Staphylococcus aureus) and gram-negative (Escherichia coli) strains. The potential of these patches in the development of novel wound dressings is highlighted by the excellent biocompatibility with fibroblast cells maintained for up to 7 days. Keywords: antibiotic; wound dressing; biocompatibility; antimicrobial activity, drug deliveryPublication Open Access Development of gallic acid-loaded ethylcellulose fibers as a potential wound dressing material(2023-09-18) ŞAHİN, ALİ; Croitoru A., Ayran M., Altan E., Karacelebi Y., Ulag S., Sahin A., Guncu M. M., Aksu B., Gunduz O., Tihăuan B., et al.In this study, novel fibers were designed based on ethylcellulose (EC), loaded with different concentrations of gallic acid (GA) using the electrospinning technique, in order to investigate the potential of these materials as wound dressings. The chemical structure and morphology, along with the antimicrobial and biocompatibility tests of the EC_GA fibers were investigated. To observe the chemical interactions between the components, fourier transform infrared spectroscopy (FTIR) was used. The morphological analyzes were performed using scanning electron microscope (SEM). The uniaxial tensile test machine was used to obtain mechanical performance of the fibers. MTT assay was applied to get the biocompatibility properties of the fibers and antimicrobial test was applied to obtain the antimicrobial activity of the fibers. Based on the obtained results, the highest viability value of 67.4% was obtained for 10%EC_100GA on the third day of incubation, demonstrating that with the addition of a higher concentration of GA, the cell viability increases. The antimicrobial tests, evaluated against Staphylococcus (S.) aureus, Escherichia (E.) coli, Pseudomonas (Ps.) aeruginosa and Candida (C.) albicans, showed a >90% microbial reduction capacity correlated with a logarithmic reduction ranging from 0.63 to 1, for 10%EC_100 GA. In vitro release tests of GA from the fibers showed that GA was totally released from 10%EC_100 GA fibers after 2880 minutes, demonstrating a controlled release profile. These findings demonstrated that EC_GA fibers may be suitable for application in biomedical fields such as wound dressing materials. However, further studies should be performed to increase the biocompatibility properties of the fibers.Publication Open Access Does decision tree analysis predict oral ulcer activity-related factors in patients with Behçet's syndrome?(2023-10-01) ÇANDERELİ, ZEHRA ÖZGE; ŞİŞMAN KİTAPÇI, NUR; AKSOY, AYSUN; ŞAHİN, ALİ; ALİBAZ ÖNER, FATMA; İNANÇ, GÜZİDE NEVSUN; DİRESKENELİ, RAFİ HANER; MUMCU, GONCA; ÇANDERELİ Z. Ö., Arslan T., Özdamar Ö., Yay M., Karaçayli Ü., ŞİŞMAN KİTAPÇI N., Adesanya A., AKSOY A., Belem J. M. F. M., Taş M. N., et al.OBJECTIVES: The study aimed to identify the interactions among treatment protocols and oral ulcer activity related factors in patients with Behçet\"s syndrome (BS) using the Classification and Regression Tree (CART) algorithm. METHODS: In this cross-sectional study, 979 patients with BS were included from16 centres in Turkey, Jordan, Brazil and the United Kingdom. In the CART algorithm, activities of oral ulcer (active vs. inactive), genital ulcer (active vs. inactive), cutaneous involvement (active vs. inactive), musculoskeletal involvement (active vs. inactive), gender (male vs. female), disease severity (mucocutaneous and musculoskeletal involvement vs. major organ involvement), smoking habits (current smoker vs. non-smoker), tooth brushing habits (irregular vs. regular), were input variables. The treatment protocols regarding immunosuppressive (IS) or non-IS medications were the target variable used to split from parent nodes to purer child nodes in the study. RESULTS: In mucocutaneous and musculoskeletal involvement (n=538), the ratio of IS use was higher in patients with irregular toothbrushing (ITB) habits (27.1%) than in patients with regular toothbrushing (RTB) habits (14.2%) in oral ulcer activity. In major organ involvement (n=441), male patients with ITB habits were more likely treated with IS medications compared to those with RTB habits (91.6% vs. 77.6%, respectively). CONCLUSIONS: Male BS patients on IS who have major organ involvement and oral ulcer activity with mucocutaneous and musculoskeletal involvement have irregular toothbrushing habits. Improved oral hygiene practices should be considered to be an integral part for implementing patient empowerment strategies for BS.Publication Open Access Fabrication and in vitro characterization of polycaprolactone/graphene oxide/collagen nanofibers for myocardial repair(2023-01-01) ULAĞ, SONGÜL; ŞAHİN, ALİ; DUMLUDAĞ, FATİH; GÜNDÜZ, OĞUZHAN; Karapehlivan S. S., Danisik M. N., Akdag Z., Yildiz E. N., Okoro O. V., Nie L., Shavandi A., ULAĞ S., ŞAHİN A., DUMLUDAĞ F., et al.This study is focused on fabricating tissue-engineered electrospun nanofibers that contain polycaprolactone (PCL), graphene oxide (GO), and collagen (COL) to get an alternative treatment for cardiac injuries. GO (1.5 wt%) is used to support the contraction-elongation of cardiomyocytes by improving electrical stimulation. The COL (1, 3, and 5 wt%) is the main component of the myocardial extracellular matrix have led to their frequent use in cardiac tissue engineering (CTE). The scanning electron microscope (SEM) images show the homogeneous and bead-free morphologies of the nanofibers. Adding a high amount (3% and 5%) of COL decreases the tensile strength value of 17% PCL/1.5% GO nanofiber. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay demonstrates that the COL addition increases cell viability compared to that in 17% PCL/1.5% GO nanofibers on the third day. The response of the nanofibers to alternating current (AC) signal is studied between the frequencies 40 and 105 Hz. The direct current (DC) conductivity values of the films are determined to be between 1.10−10 and 6.10−10 S m−1 at 25 °C. The AC conductivity values show frequency-dependent behavior. Among the PCL/GO-based electrospun nanofibers, 17% PCL/1.5% GO/5% COL nanofiber shows greater DC and AC conductivity than 17% PCL/1.5% GO nanofiber.