Person:
AKSU, MEHMET BURAK

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

OrgUnit Logo
Organizational Unit

Job Title

Last Name

AKSU

First Name

MEHMET BURAK

Name

Filters

2022 - 20235
Reset filters

Settings

Has files: true × Subject: CLINICAL MEDICINE ×

Search Results

Now showing 1 - 5 of 5
  • Thumbnail Image
    PublicationOpen Access
    FIP teşhisi konmuş kedilerden elde edilen periferal kan mononükleer hücreler ile kedi overinden elde edilen mezenkimal kök hücrenin immünolojik araştırılması
    (2023-05-25) AKSU, MEHMET BURAK; AKKOÇ, TUNÇ; Güven N., Erhan M. N., Karayusuf K., Doğanay K., Kılıç S., Tunca Z., Aksu M. B., Akkoç T.
    Giriş ve Amaç: Coronavirüsler hem insanlarda hem de hayvanlarda hafiften son derece şiddetli enfeksiyonlara kadar değişkenlik göstermektedir. Coronaviruslar çoğunlukla gastroentestinal ve solunum sistemi enfeksiyonlarına neden olurken bazıları ensefaliti ve hepatite neden olmaktadır. Kedilerin Enfeksiyöz Peritonitisi (FIP) ise kedi coronavirüslerinin (FCoV) neden olduğu bir enfeksiyondur. Çalışmamızda ise kedi ovaryum kaynaklı mezenkimal kök hücrelerin (O-MKH), FIP teşhisi konmuş kedilerden elde edilen periferal kan mononükleer hücrelere (PKMH) etkisinin in vitro ortamda araştırılıp değerlendirilmesi amaçlanmaktadır. Gereç ve Yöntem: Projemizde veteriner kliniğinde FIP tanısı konulmuş 5-13 yaşlarındaki kediler çalışmaya dahil edilecektir. Bu süreçte veteriner kliniğine gelen hastalardan FIP pozitif çıkan kedilerden hasta sahiplerinden alınan onam sonrasında 5cc periferal kan örneği alınacak ve Marmara Üniversitesi Tıp Fakültesi immünoloji anabilim dalında PKMH izole edilecektir. Ayrıca, kedi ovaryumlardan MKH izolasyonu gerçekleştirilecektir. İzole edilen PKMH ile O-MKH\"ların (+/-) ko-kültür işlemleri gerçekleştirilecektir. 5 günlük kültür işleminden sonra O-MKH\"nin FIP hasta PMKH\"larının canlılıkları üzerindeki etkileri akım sitometri yöntemi ile analiz edilecektir. Gerekli etik ve kurum izinleri alınmıştır. Bulgular: Yapılan ko-kültür çalışması sonrasında akım sitometri yöntemi ile PKMH’lerin canlılıklarındaki değişimler kıyaslanmıştır. Yapılan analizler sonucunda FIP kedilerin PKMH’ları MKH’nın olmadığı durumlarda canlılık oranı 55.6 (±5.2) iken MKH varlığında canlılıklarının 70.13 (±2.1)’e yükseldiği görülmüştür. Buna ek olarak, apoptoz geçiren PKMH’ların oranı MKH yokluğunda 34.7 (±4.4) iken MKH varlığında 12.7’e (±1.8) düştüğü gözlenmiştir. Hastalardaki PKMH’ların sağlıklı kedilerin durumları ile kıyaslandığında ise FIP hastası PKMH’ların canlılıkları MKH varlığında sağlıklı kedilerin PKMH’ların canlılık seviyesine yükseldiği görülmüştür. Sonuç: FIP hastalığına sahip olan kedilerin PKMH’larındaki canlılık oranının sağlıklı kediye kıyasla canlılık oranının beklendiği gibi daha düşük olduğu görülmüştür. Yine de MKH’nın varlığında FIP hastası kedilerin PKMH’larındaki canlılık seviyesinin sağlıklı kedilerin PKMH’lerin canlılık oranına yükseldiği görülmüştür. Ayrıca, MKH’ların apoptoz geçiren PKMH’ları FIP hastası kedilerde düşürdüğü gözlenmiştir. Elde edilen bu veriler çerçevesinde MKH’ların FIP hastalarında olumlu etkisi olduğu gözlenmektedir.
  • Thumbnail Image
    PublicationOpen Access
    The failure on the effectiveness of formalin on cadaver disinfection and alternative methods
    (2023-01-01) AKSU, MEHMET BURAK; ÖZKAN, MAZHAR; ŞEHİRLİ, ÜMİT SÜLEYMAN; Yanilmaz O., Guncu M. M., AKSU M. B., ÖZKAN M., ŞEHİRLİ Ü. S.
    Objective: It was aimed to identify the contaminant and determine the alternative disinfectant detection in the microbial growth observed in various parts of the cadaver stored in the formalin tank in the dissection laboratory of Marmara University Anatomy Department. We also performed a literature review of this unusual pathogen. Materials and Methods: Swab samples were inoculated on agar mediums. After incubation, matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS) analysis was used to identify the isolate from the detected uniform colonies. Sample solution from the cadaver tank, freshly prepared 5% formalin and 0.55% ortho-phthalaldehyde were used to determine the disinfectant sensitivity of the isolate. Results: According to 16s rDNA sequence analysis, it was concluded as Skermanella aerolata with 99% similarity. In the disinfectant susceptibility test, it was observed that S. aerolata and control bacteria could grow in 5% formalin taken from the cadaver tank. No growth was detected in other disinfectants. Conclusion: To prevent cadaver contamination in anatomy laboratories, the quality control of the embalming solutions and indoor air filtration of the dissection rooms should be checked at regular intervals. Members of Skermanella genus have been identified as environmental organisms in several studies, however, recent researches reported this bacterium as a human pathogen.
  • Thumbnail Image
    PublicationOpen Access
    Distribution and drug resistance of pathogens isolated from patients with hematological malignancies in three-year period
    (2022-09-01) AKSU, MEHMET BURAK; Arabacı Ç., Aksu M. B.
    Objective: This study aimed to retrospectively analyze the 3-year findings of bacterial and fungal pathogens isolated from infections in patients with hematological malignancies.Methods: A retrospective analysis of 158 patients with hematological malignancies treated between January 2015 and December 2017 in Okmeydanı Training and Research Hospital, Istanbul, Turkey. A total of 3374 non-consecutive blood samples (n=1954) from 158 patients, urine samples (n=1024), wound swabs (n=94), respiratory samples (n=87), and other samples (CSF, body fluids, etc.) (n=215) were collected. Results: Pathogen growth was detected in 6% (203/3374) of the samples. The most frequently isolated pathogens are coagulase-negative staphylococci (CNS, 20%), E. coli (19%), Klebsiella sp. (17%), and yeasts (16%), followed by Pseudomonas sp., Acinetobacter sp. and Enterococcus sp. (7%, 6%, and 6%, respectively). Candida species were dominant in fungal isolates (26/32; 81.2%). The most commonly detected antibiotic resistance patterns and organisms are carbapenem-resistant Acinetobacter sp. (92%), methicillin-resistant CNS (83%), carbapenem-resistant Klebsiella sp. (65%), MRSA (57%), and vancomycin-resistant Enterococci (VRE, 42%). Conclusion: Bloodstream infections accounted for more than half of all infection episodes. Periodic examination of the clinical and microbiological profiles of infections developing in patients with malignancy is essential for successful treatment management.
  • Thumbnail Image
    PublicationOpen Access
    Low-dose valacyclovir use with preemptive monitoring in kidney transplant recipients with intermediate cytomegalovirus infection risk
    (2022-01-01) VELİOĞLU, ARZU; ARIKAN, İZZET HAKKI; AŞICIOĞLU, EBRU; TUĞLULAR, ZÜBEYDE SERHAN; AKSU, MEHMET BURAK; VELİOĞLU A., Alagoz S., Atas D. B. , ARIKAN İ. H. , AŞICIOĞLU E., AKSU M. B. , Seyahi N., TUĞLULAR Z. S.
    PROP
  • Thumbnail Image
    PublicationOpen Access
    Assessment of Different Imaging Systems for Dental Plaque Scoring: an in-vivo study
    (2022-06-13) MEŞELİ, SİMGE; EKENEL, SERGEN; AKSU, MEHMET BURAK; YANIKOĞLU, FUNDA; TAĞTEKİN, DİLEK; MEŞELİ S., EKENEL S., AKSU M. B., TAĞTEKİN D., YANIKOĞLU F.
    Aim: The aim of the clinical study was to analyze the correlation between the dental plaque scores measuredby different imaging systems.Materials and Methods: Thirty volunteers between the age of 18-30, who visited Marmara University, Facultyof Dentistry whose DMFT scores were under 4, were included in the study. Dental plaque on anterior teeth’sbuccal surfaces of the volunteers were scored using different imaging systems after staining with a disclosinggel. The patients were divided into three groups. The groups were given two different herbal toothpastes(ginger and hemp) and a conventional toothpaste. Digital photography, intraoral digital scanning (iTeroElements 2) and FluoreCam were used for taking intraoral images. The Turesky Modified Quigley Hein PlaqueIndex (TMQHPI) was used for the clinical examination and the taken images. The correlations between theclinical examination and imaging scores were analyzed before and after using the toothpastes for 21 days.Shapiro Wilk Test and Pearson’s correlation were performed using IBM SPSS Statistics V23 (IBM Co. , Armonk,USA) (p<0.05).Results: The lowest mean value was obtained in the clinical examination without disclosing gel method andthe highest mean value was obtained in the FluoreCam method. The mean value of the clinical examinationwithout disclosing gel was found to be lower than the clinical examination with disclosing gel method andintraoral digital scanning method. There was no significant difference between toothpastes before and aftertoothbrushing for all clinical imaging methods.Conclusion: Staining of the plaque and the use of digital imaging systems have shown a positive effect inthe determination of dental plaque. Intraoral imaging systems may be used as an alternative to supplementthe shortcomings of conventional clinical indices for educating patients about plaque control and continuedpatient oral care. Herbal toothpastes are just as effective as conventional toothpastes in removing dentalplaque.