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Nonhydrolytic sol-gel synthesized oligosiloxane resin reinforced thiol-ene photocured coatings for the immobilization of acetylcholinesterase

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dc.contributor.authorÇAKMAKÇI, EMRAH
dc.contributor.authorsCakmakci, Emrah; Demir, Serap
dc.date.accessioned2022-03-12T22:27:58Z
dc.date.available2022-03-12T22:27:58Z
dc.date.issued2019
dc.description.abstractAcetylcholinesterase (AChE), which is responsible for the hydrolysis of neurotransmitter acetylcholine, is a critical enzyme for the nervous system and also a biomarker for organophosphorous pesticide detection. The immobilization of AChE is an active area of research and recently the use of sol-gel-derived materials for enzyme immobilization has gained a lot of attraction. In this work, AChE was covalently immobilized onto a photocured substrate which was reinforced with an oligosiloxane resin. The oligosiloxane resin was designed to have both vinyl and epoxide groups and prepared via nonhydrolytic sol-gel technique. The strategy employed in this study offered a platform that has good mechanical and thermal properties and also suitable for modification. Thus, AChE was also immobilized onto these substrates after amine modification of the epoxy groups and followed by glutaraldehyde activation. Over 80% enzyme immobilization yield was achieved. At certain pH values (5.5 and 8.5) and under relatively higher temperatures (above 40 degrees C) the immobilized enzymes were found to have higher catalytic activity than the free enzyme. Furthermore, by immobilization the reuse and the storage stability of the enzyme was improved and the stability of the immobilized enzyme against the inhibitory effects of certain metal cations was enhanced [GRAPHICS] . Nonhydrolytic sol-gel synthesized oligosiloxane resin reinforced thiol-ene photocured coatings for the immobilization of acetylcholinesterase. Emrah CAKMAKCI, Serap DEMIR. HighlightsAn oligosiloxane resin was prepared via nonhydrolytic sol-gel technique.The oligosiloxane resin was used to reinforce thiol-ene photocured coatings.Acetylcholinesterase was immobilized onto the photocured coatings.By immobilization, storage stability, reuse and metal ion resistance were improved.
dc.identifier.doi10.1007/s10971-019-05006-2
dc.identifier.eissn1573-4846
dc.identifier.issn0928-0707
dc.identifier.urihttps://hdl.handle.net/11424/235265
dc.identifier.wosWOS:000470682300008
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofJOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectThiol-ene
dc.subjectNonhydrolitic sol-gel
dc.subjectAcetylcholinesterase
dc.subjectEnzyme immobilization
dc.subjectCOVALENT IMMOBILIZATION
dc.subjectPASSIVATION LAYER
dc.subjectALPHA-AMYLASE
dc.subjectFUNCTIONALIZATION
dc.subjectMEMBRANES
dc.subjectLIPASE
dc.subjectEPOXY
dc.titleNonhydrolytic sol-gel synthesized oligosiloxane resin reinforced thiol-ene photocured coatings for the immobilization of acetylcholinesterase
dc.typearticle
dspace.entity.typePublication
local.avesis.id897f5270-3105-402e-9513-af09edb1f4d1
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages10
local.journal.quartileQ2
oaire.citation.endPage81
oaire.citation.issue1
oaire.citation.startPage72
oaire.citation.titleJOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
oaire.citation.volume91
relation.isAuthorOfPublication332869cd-e8a9-40ac-b678-cf5b01c6a6dc
relation.isAuthorOfPublication.latestForDiscovery332869cd-e8a9-40ac-b678-cf5b01c6a6dc

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