Publication:
Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome

Simple item page
dc.contributor.authorTURAN, KADİR
dc.contributor.authorsTuran, K; Mibayashi, M; Sugiyama, K; Saito, S; Numajiri, A; Nagata, K
dc.date.accessioned2022-03-14T10:54:32Z
dc.date.available2022-03-14T10:54:32Z
dc.date.issued2004-01-21
dc.description.abstractMx proteins belong to the dynamin superfamily of high molecular weight GTPases and interfere with multiplication of a wide variety of viruses. Earlier studies show that nuclear mouse Mx1 and human MxA designed to be localized in the nucleus inhibit the transcription step of the influenza virus genome. Here we set a transient influenza virus transcription system using luciferase as a reporter gene and cells expressing the three RNA polymerase subunits, PB1, PB2 and PA, and NP. We used this reporter assay system and nuclear-localized MxA proteins to get clues for elucidating the anti-influenza virus activity of MxA. Nuclear-localized VP16-MxA and MxA-TAg NLS strongly interfered with the influenza virus transcription. Over-expression of PB2 led to a slight resumption of the transcription inhibition by nuclear MxA, whereas over-expression of PB1 and PA did not affect the MxA activity. Of interest is that the inhibitory activity of the nuclear MxA was markedly neutralized by over-expression of NP. An NP devoid of its C-terminal region, but containing the N-terminal RNA binding domain, also neutralized the VP16-MxA activity in a dose-dependent manner, whereas an NP lacking the N-terminal region did not affect the VP16-MxA activity. Further, not only VP16-MxA but also the wild-type MxA was found to interact with NP in influenza virus-infected cells. This indicates that the nuclear MxA suppresses the influenza virus transcription by interacting with not only PB2 but also NP.
dc.identifier.doi10.1093/nar/gkh192
dc.identifier.eissn1362-4962
dc.identifier.issn0305-1048
dc.identifier.pubmed14752052
dc.identifier.urihttps://hdl.handle.net/11424/245427
dc.identifier.wosWOS:000188989000035
dc.language.isoeng
dc.publisherOXFORD UNIV PRESS
dc.relation.ispartofNUCLEIC ACIDS RESEARCH
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMESSENGER-RNA SYNTHESIS
dc.subjectEXPRESSING HUMAN MXA
dc.subjectTHOGOTO-VIRUS
dc.subjectMAMMALIAN-CELLS
dc.subjectGTP-BINDING
dc.subjectMURINE MX1
dc.subjectA VIRUS
dc.subjectINTERFERON
dc.subjectPOLYMERASE
dc.subjectGENE
dc.titleNuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome
dc.typearticle
dspace.entity.typePublication
local.avesis.id4d7113af-6e65-43c5-94fb-f9e328a6b206
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages10
oaire.citation.endPage652
oaire.citation.issue2
oaire.citation.startPage643
oaire.citation.titleNUCLEIC ACIDS RESEARCH
oaire.citation.volume32
relation.isAuthorOfPublication9cc23fc2-5031-46cd-91a0-a2e13ab788d4
relation.isAuthorOfPublication.latestForDiscovery9cc23fc2-5031-46cd-91a0-a2e13ab788d4

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Turan - 2004 - Nuclear MxA proteins form a complex with influenza.pdf
Size:
312.66 KB
Format:
Adobe Portable Document Format
Download

Collections

Research Outputs