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What Are the Multi-Omics Mechanisms for Adaptation by Microorganisms to High Alkalinity? A Transcriptomic and Proteomic Study of a Bacillus Strain with Industrial Potential

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dc.contributor.authorSAYAR, AHMET ALP
dc.contributor.authorsKaya, Fatma Ece Altinisik; Avci, Fatma Gizem; Sayar, Nihat Alpagu; Kazan, Dilek; Sayar, Ahmet Alp; Akbulut, Berna Sariyar
dc.date.accessioned2022-03-12T22:25:05Z
dc.date.available2022-03-12T22:25:05Z
dc.date.issued2018
dc.description.abstractAlkaliphilic organisms are among an industrially important class of extremophile microorganisms with the ability to thrive at pH 10-11.5. Microorganisms that exhibit alkaliphilic characteristics are sources of alkali-tolerant enzymes such as proteases, starch degrading enzymes, cellulases, and metabolites such as antibiotics, enzyme inhibitors, siderophores, organic acids, and cholic acid derivatives, which have found various applications in industry for human and environmental health. Yet, multi-omics mechanisms governing adaptation to high alkalinity have been poorly studied. We undertook the present work to understand, as a case study, the alkaliphilic adaptation strategy of the novel microorganism, Bacillus marmarensis DSM 21297, to alkaline conditions using a multi-omics approach that employed transcriptomics and proteomics. As alkalinity increased, bacteria remodeled the peptidoglycan layer by changing peptide moieties along with the peptidoglycan constituents and altered the cell membrane to reduce lipid motility and proton leakiness to adjust intracellular pH. Different transporters also contributed to the maintenance of this pH homeostasis. However, unlike in most well-known alkaliphiles, not only sodium ions but also potassium ions were involved in this process. Interestingly, increased pH has triggered the expression of neither general stress proteins nor gene encoding proteins associated with heat, salt, and nutrient stresses. Only an increase in the expression of oxidative stress related genes was evident. Endospore formation, also a phenomenon closely linked to stress, was unclear. This questioned if high pH was a real stress for B. marmarensis. These new findings, corroborated using the multi-omics approach of the present case study, broaden the knowledge on the mechanisms of alkaliphilic adaptation and might also potentially offer useful departure points for further industrial applications with other microorganisms.
dc.identifier.doi10.1089/omi.2018.0127
dc.identifier.eissn1557-8100
dc.identifier.issn1536-2310
dc.identifier.pubmed30457468
dc.identifier.urihttps://hdl.handle.net/11424/234872
dc.identifier.wosWOS:000450686100005
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INC
dc.relation.ispartofOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBacillus marmarensis
dc.subjectalkaliphilic adaptation
dc.subjecttranscriptomics
dc.subjectproteomics
dc.subjectmulti-omics
dc.subjectsystems and industrial biology
dc.subjectALKYL HYDROPEROXIDE REDUCTASE
dc.subjectMECHANOSENSITIVE CHANNELS
dc.subjectALKALIPHILIC BACILLUS
dc.subjectNA+/H+ ANTIPORTER
dc.subjectPROTON
dc.subjectPROTECTION
dc.subjectBACTERIUM
dc.subjectPROTEINS
dc.subjectFAMILIES
dc.subjectSUBTILIS
dc.titleWhat Are the Multi-Omics Mechanisms for Adaptation by Microorganisms to High Alkalinity? A Transcriptomic and Proteomic Study of a Bacillus Strain with Industrial Potential
dc.typearticle
dspace.entity.typePublication
local.avesis.id1ef1fe80-d375-473e-847c-5f7f522488c2
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages16
local.journal.quartileQ2
oaire.citation.endPage732
oaire.citation.issue11
oaire.citation.startPage717
oaire.citation.titleOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
oaire.citation.volume22
relation.isAuthorOfPublication279446ed-107e-4bcc-b5a2-21a8f8fbc72c
relation.isAuthorOfPublication.latestForDiscovery279446ed-107e-4bcc-b5a2-21a8f8fbc72c

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