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Grape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction

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dc.contributor.authorDULUNDU, ENDER
dc.contributor.authorsDulundu, Ender; Ozel, Yahya; Topaloglu, Umit; Toklu, Hale; Ercan, Feriha; Gedik, Nursal; Sener, Goksel
dc.date.accessioned2022-03-12T17:32:57Z
dc.date.available2022-03-12T17:32:57Z
dc.date.issued2007
dc.description.abstractBackground and aim: The aim of this study was to assess the protective effect of grape seed extract (GSE) against oxidative liver injury and fibrosis induced by biliary obstruction in rats. Methods: Wistar albino rats were divided into four groups; control (C), GSE-treated, bile duct ligated (BDL), and BDL and GSE-treated (BDL + GSE) groups. GSE was administered at a dose of 50 mg/kg a day orally for 28 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels were determined to assess liver function and tissue damage, respectively. Tumor necrosis factor-alpha (TNF-alpha) and antioxidant capacity (AOC) were assayed in plasma samples. Liver tissues were taken for determination of the hepatic malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen content. Production of reactive oxidants was monitored by chemiluminescence (CL) assay. Results: Serum AST, ALT, LDH and plasma TNF-alpha were elevated in the BDL group as compared to the control group and were significantly decreased with GSE treatment. Plasma AOC and hepatic GSH level, depressed by BDL, was elevated back to the control level in the GSE-treated BDL group. Increases in tissue MDA level, MPO activity and collagen content due to BDL were also attenuated by GSE treatment. Furthermore, luminol and lucigenin CL values in the BDL group increased dramatically compared to the control and were reduced by GSE treatment. Discussion: These results suggest that GSE protects the liver from oxidative damage following bile duct ligation in rats. This effect possibly involves the inhibition of neutrophil infiltration and lipid peroxidation; thus, restoration of oxidant and antioxidant status in the tissue.
dc.identifier.doi10.1111/j.1440-1746.2007.04875.x
dc.identifier.issn0815-9319
dc.identifier.pubmed17565645
dc.identifier.urihttps://hdl.handle.net/11424/228734
dc.identifier.wosWOS:000247205600022
dc.language.isoeng
dc.publisherBLACKWELL PUBLISHING
dc.relation.ispartofJOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbile duct ligation
dc.subjectgrape seed
dc.subjecthepatic fibrosis
dc.subjectlipid peroxidation
dc.subjectrat
dc.subjectPROANTHOCYANIDIN EXTRACT
dc.subjectHEPATIC-FIBROSIS
dc.subjectLIVER FIBROSIS
dc.subjectANTIOXIDANT ACTIVITY
dc.subjectFREE-RADICALS
dc.subjectIN-VIVO
dc.subjectRATS
dc.subjectACTIVATION
dc.subjectINJURY
dc.subjectMYELOPEROXIDASE
dc.titleGrape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction
dc.typearticle
dspace.entity.typePublication
local.avesis.ide3f5f3af-f627-4549-90f6-2fa6e50b01ba
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages8
oaire.citation.endPage892
oaire.citation.issue6
oaire.citation.startPage885
oaire.citation.titleJOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
oaire.citation.volume22
relation.isAuthorOfPublicationedcd2255-94d5-4f56-b0c8-1385c55279b0
relation.isAuthorOfPublication.latestForDiscoveryedcd2255-94d5-4f56-b0c8-1385c55279b0

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