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Ghrelin alleviates biliary obstruction-induced chronic hepatic injury in rats

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dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsIseri, Sevgln Oezlem; Sener, Goeksel; Saglam, Beyhan; Ercan, Feriha; Gedik, Nursal; Yegen, Berrak C.
dc.date.accessioned2022-03-12T17:34:30Z
dc.date.available2022-03-12T17:34:30Z
dc.date.issued2008
dc.description.abstractBackground: Reactive oxygen species and oxidative stress are implicated in hepatic stellate cell activation and liver fibrosis, which are initiated by recruitment of inflammatory cells and by activation of cytokines. Objective: The possible anti-oxidant and anti-inflammatory effects of ghrelin were evaluated in a hepatic fibrosis model in rats with bile duct ligation (BDL). Methods: Under anesthesia, bile ducts of Sprague Dawley rats were ligated, and half of the rats were subcutaneously administered with ghrelin (10 ng/kg/day) and the rest with saline for 28 days. Sham-operated control groups were administered saline or ghrelin. On the 28th day of the study, rats were decapitated and malondialdehyde (NIDA) content - an index of lipid peroxidation, and myeloperoxidase (MPO) activity - an index of neutrophil infiltration - were determined in the liver tissues. Oxidant-induced tissue fibrosis was determined by collagen contents, while the hepatic injury was analyzed microscopically. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels and lactate dehydrogenase (LDH) levels were determined to assess liver function and tissue damage, respectively. Pro-inflammatory cytokines; TNF-alpha, IL-1 beta and IL-6 were also assayed in plasma samples. Results: In the saline-treated BDL group, hepatic NIDA levels, MPO activity and collagen content were increased (p < 0.001), suggesting oxidative organ damage, as confirmed histologically. In the ghrelin-treated BDL group, however, all of the oxidant responses were reversed significantly (p < 0.05-p < 0.001). Serum AST, ALT, LDH levels, and cytokines were elevated in the BDL group as compared to the control group, while this increase was significantly decreased by ghrelin treatment. Conclusion: Owing to the anti-inflammatory and anti-oxidant effect as demonstrated in our study, it is possible to speculate that exogenously administered ghrelin may possess an antifibrotic effect against biliary obstruction-induced liver fibrosis. Thus, it seems likely that ghrelin may be of potential therapeutic value in protecting the liver fibrosis and oxidative injury due to biliary obstruction. (c) 2007 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.regpep.2007.08.014
dc.identifier.eissn1873-1686
dc.identifier.issn0167-0115
dc.identifier.pubmed17884193
dc.identifier.urihttps://hdl.handle.net/11424/229029
dc.identifier.wosWOS:000253278600011
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofREGULATORY PEPTIDES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbile duct ligation
dc.subjectghrelin
dc.subjecthepatic fibrosis
dc.subjectlipid peroxidation
dc.subjectPROINFLAMMATORY CYTOKINES
dc.subjectACYLATED PEPTIDE
dc.subjectACTIVATION
dc.subjectFIBROSIS
dc.subjectMYELOPEROXIDASE
dc.subjectANGIOGENESIS
dc.subjectCIRRHOSIS
dc.subjectDAMAGE
dc.titleGhrelin alleviates biliary obstruction-induced chronic hepatic injury in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.ide8f13c31-1bc3-4bd7-90f0-e60037e9e218
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage79
oaire.citation.issue1-3
oaire.citation.startPage73
oaire.citation.titleREGULATORY PEPTIDES
oaire.citation.volume146
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

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