Publication:
Antioxidant Agent Quercetin Prevents Impairment of Bladder Tissue Contractility and Apoptosis in a Rat Model of Ischemia/Reperfusion Injury

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dc.contributor.authorTİNAY, İLKER
dc.contributor.authorsTinay, Ilker; Sener, Tarik E.; Cevik, Ozge; Cadirci, Selin; Toklu, Hale; Cetinel, Sule; Sener, Goeksel; Tarcan, Tufan
dc.date.accessioned2022-03-12T20:32:40Z
dc.date.available2022-03-12T20:32:40Z
dc.date.issued2017
dc.description.abstractObjectivesTo examine the possible protective effect of quercetin (QT), which is well known for its antioxidant and protective effects in circumstances of oxidative stress, on urinary bladder tissue in a rat model of ischemia/reperfusion (I/R) injury, which is a known factor for the development of lower urinary tract dysfunction partly mediated by the generation of free radicals causing oxidative damage. MethodsThirty male Sprague-Dawley rats were subjected to I/R injury through clamping the abdominal aorta for 30 min and then allowing reperfusion for the next 60 min. Quercetin (20 mg/kg; subcutaneously) or vehicle were given before ischemia and just before reperfusion. Findings of the isometric contraction studies in the organ bath and of the histological examinations along with oxidative stress markers were evaluated in bladder tissues. ResultsIncreased malondialdehyde (MDA) levels and myeloperoxidase (MPO) activities and decreased glutathione (GSH) levels and superoxide dismutase (SOD) activities in the I/R group were reduced by QT treatment. In the I/R group, pro-apoptotic marker caspase-3 was increased and anti-apoptotic bcl-2 protein was decreased, while QT treatment significantly reversed these parameters. In the I/R group contractile responses of the bladder strips to carbachol were significantly lower than those of the control group, which were reversed by QT treatment. ConclusionQuercetin treatment protects bladder tissue contractility against acute I/R injury by decreasing oxidative stress and apoptosis induced by I/R.
dc.identifier.doi10.1111/luts.12125
dc.identifier.eissn1757-5672
dc.identifier.issn1757-5664
dc.identifier.pubmed28394499
dc.identifier.urihttps://hdl.handle.net/11424/234421
dc.identifier.wosWOS:000398862500009
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofLUTS-LOWER URINARY TRACT SYMPTOMS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectischemia reperfusion injury
dc.subjectquercetin
dc.subjecturinary bladder
dc.subjectISCHEMIA-REPERFUSION INJURY
dc.subjectSUPEROXIDE-DISMUTASE ACTIVITY
dc.subjectSPINAL-CORD-INJURY
dc.subjectURINARY-BLADDER
dc.subjectFUNCTIONAL CONSEQUENCES
dc.subjectBIOCHEMICAL-CHANGES
dc.subjectOXIDANT DAMAGE
dc.subjectNITRIC-OXIDE
dc.subjectISCHAEMIA/REPERFUSION
dc.subjectDYSFUNCTION
dc.titleAntioxidant Agent Quercetin Prevents Impairment of Bladder Tissue Contractility and Apoptosis in a Rat Model of Ischemia/Reperfusion Injury
dc.typearticle
dspace.entity.typePublication
local.avesis.ide8c3e4dc-3028-4b6a-a5d4-8fc081859153
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
local.journal.quartileQ4
oaire.citation.endPage123
oaire.citation.issue2
oaire.citation.startPage117
oaire.citation.titleLUTS-LOWER URINARY TRACT SYMPTOMS
oaire.citation.volume9
relation.isAuthorOfPublicationf54b9b38-576e-4b79-8b43-647b1e25292c
relation.isAuthorOfPublication.latestForDiscoveryf54b9b38-576e-4b79-8b43-647b1e25292c

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