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In vitro effects of nitric oxide donors on apoptosis and oxidative/nitrative protein modifications in ADP-activated platelets

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dc.contributor.authorŞENER, AZİZE
dc.contributor.authorsSener, A.; Egemen, G.; Cevik, O.; Yanikkaya-Demirel, G.; Apikoglu-Rabus, S.; Ozsavci, D.
dc.date.accessioned2022-03-12T18:09:10Z
dc.date.available2022-03-12T18:09:10Z
dc.date.issued2013
dc.description.abstractNitric oxide (NO) is an important physiological signaling molecule. However, when produced in excessive amounts, NO can also have toxic effects. The aim of this study is to investigate the effects of exogenous- and endogenous-derived NO on oxidative modifications of proteins and apoptosis in activated platelets. Washed platelets were incubated with L-arginine or nitroso-glutathione (GSNO) in the presence of adenosine diphosphate (ADP). After incubation, caspase-3 activity, phosphatidylserine (PS) externalization and the potential of mitochondrial membrane as markers of apoptosis were measured. In addition, the alterations in protein carbonylation (PCO) and nitrotyrosine (NT) formation as markers of protein oxidation were examined. Platelet activation with ADP (20 p,M) significantly increased PCO and NT levels and apoptotic events. After incubation with L-arginine, platelet NO production increased significantly. This L-arginine-induced increase caused decreases in formerly increased PCO and NT levels associated with ADP-induced platelet activation. Stimulation of NO production with L-arginine protected platelets from apoptosis. GSNO caused an increase in protein NT levels. Despite this change, GSNO was effective in inhibition of P-selectin expression, platelet aggregation, protein carbonylation and apoptosis. The results suggest that L-arginine and GSNO-mediated NO leads to the inhibition of key apoptotic processes including caspase-3 activation, PS exposure and low mitochondrial membrane potential in washed platelets. The inhibitory effect of platelet clearance of L-arginine and GSNO may be a novel useful therapeutic property in clinical application.
dc.identifier.doi10.1177/0960327112455673
dc.identifier.eissn1477-0903
dc.identifier.issn0960-3271
dc.identifier.pubmed23111882
dc.identifier.urihttps://hdl.handle.net/11424/231246
dc.identifier.wosWOS:000316841000001
dc.language.isoeng
dc.publisherSAGE PUBLICATIONS LTD
dc.relation.ispartofHUMAN & EXPERIMENTAL TOXICOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNitric oxide
dc.subjectapoptosis
dc.subjectprotein oxidation
dc.subjectL-arginine
dc.subjectnitroso-glutathione
dc.subjectplatelet
dc.subjectL-ARGININE
dc.subjectS-NITROSOGLUTATHIONE
dc.subjectOXIDATIVE STRESS
dc.subjectNEURONAL CELLS
dc.subjectINHIBITION
dc.subjectSYNTHASE
dc.subjectNITRATION
dc.subjectEXPRESSION
dc.subjectPEROXYNITRITE
dc.subjectRELEASE
dc.titleIn vitro effects of nitric oxide donors on apoptosis and oxidative/nitrative protein modifications in ADP-activated platelets
dc.typearticle
dspace.entity.typePublication
local.avesis.id8cf7fb05-346c-4c4a-9246-d440495407a6
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages11
oaire.citation.endPage235
oaire.citation.issue3
oaire.citation.startPage225
oaire.citation.titleHUMAN & EXPERIMENTAL TOXICOLOGY
oaire.citation.volume32
relation.isAuthorOfPublicationfd65174e-4126-41c3-913d-e8bcdce20632
relation.isAuthorOfPublication.latestForDiscoveryfd65174e-4126-41c3-913d-e8bcdce20632

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