Publication: Anticancer effect of phthalocyanine derivatives on colorectal cancer: in vitro and in vivo model
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Date
2022-08-28
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Abstract
Colorectal cancer (CRC), also known as bowel cancer, colon cancer, or rectal cancer, develops cancer
from the colon or rectum. Although 5-Fluorouracil (5-FU) is the primary antineoplastic used in
treatment, its low treatment efficiency has led to the search for new agents. Phthalocyanines are aromatic
macrocycles that contain four iso-indoline units connected by four nitrogen atoms. Phthalocyanines
Derivatives (PD) gradually started to gain importance as anticancer agents. Our study aims to investigate
the in vitro and in vivo anticancer effects of PD and 5-FU combinations on CRC. Cytotoxicity,
genotoxicity, apoptosis, i-ROS, glutathione, calcium, and mitochondrial membrane potential were
measured in LoVo and CCD18Co cells. Cells were given to nude mice by the xenograft method. After
four weeks of single and combination therapy, tumor size was measured by in vivo imaging system
(IVIS) and caliper. It was examined biochemically and histopathologically in related serum, tissues, and
tumors.
Combination treatment in vitro dose-dependently increased cytotoxicity, iROS, genotoxicity, apoptosis,
and calcium significantly compared to a single treatment (p<0.001). DNA damage and apoptosis were
induced higher in combined treatment than single treatment and increased statistically significantly
(p<0.001). It was found that it decreased glutathione and MMP levels statistically (p<0.01; p<0.001). In
all results of in vitro studies, PD affected cancer cells more than healthy cells. Combination treatment
in vivo was found to reduce tumor size and density by a minimum of 66% at 30 days compared to a
single treatment. Our results showed that PD has different anticancer properties in vivo and in vitro. The combined use of
PD and 5-FU may be an option for routine colorectal cancer treatment.
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Keywords
Phthalocyanine Derivatives, colorectal cancer, in vitro and in vivo cancer model
Citation
GÜLER E. M., ÖZDEMİR M., YALÇIN B., KOÇYİĞİT A., \"Anticancer Effect of Phthalocyanine Derivatives on Colorectal Cancer: in vitro and in vivo model\", 2nd International Multidisciplinary Cancer Research Congress, Giresun, Türkiye, 21 - 24 Temmuz 2022