Publication:
The investigation of genetic polymorphisms in the carbonic anhydrase VI gene exon 2 and salivary parameters in type 2 diabetic patients and healthy adults

Simple item page
dc.contributor.authorYARAT, AYŞEN
dc.contributor.authorsOzturk, Leyla Koc; Ulucan, Korkut; Akyuz, Serap; Furuncuoglu, Halit; Bayer, Hikmet; Yarat, Aysen
dc.date.accessioned2022-03-12T18:06:54Z
dc.date.available2022-03-12T18:06:54Z
dc.date.issued2012
dc.description.abstractThe aim of this study was to investigate carbonic anhydrase (CA) VI Exon 2 single nucleotide polymorphism (SNP) and its possible association with salivary parameters in type 2 diabetic patients compared to healthy adults. Caries status was measured by using the DMFT (number of decayed, missing, and filled teeth) index. Unstimulated whole saliva and blood samples were taken. SNPs of CA gene exon 2 were determined by PCR and DNA sequencing. Salivary CA activity and buffering capacity were determined by the method of Verpoorte and Ericson, respectively. Furthermore, salivary pH was measured with pH paper and salivary flow rate was calculated. Salivary buffering capacity and pH were significantly lower in diabetic patients than those of healthy subjects (P < 0.05). Salivary flow rate, CA activity and DMFT levels did not differ between groups (P > 0.05). Four SNPs were detected; their pubmed database number are rs2274327 (C/T), rs2274328 (A/C), rs2274329 (G/C) and rs2274330. While first three of those were responsible for amino acid changes, the last one was not. The frequencies of SNPs were not significant between groups (P > 0.05). Positive significant correlation was found between CA activity and the frequency of SNPs. There was no correlation between the SNPs frequencies and pH or buffering capacity. SNPs found in this study may be related to salivary CA activity in diabetics.
dc.identifier.doi10.1007/s11033-011-1374-1
dc.identifier.issn0301-4851
dc.identifier.pubmed22198626
dc.identifier.urihttps://hdl.handle.net/11424/230960
dc.identifier.wosWOS:000302147800072
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofMOLECULAR BIOLOGY REPORTS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectType 2 diabetes
dc.subjectSaliva
dc.subjectCarbonic anhydrase VI
dc.subjectGene
dc.subjectPolymorphism
dc.subjectISOENZYME-VI
dc.subjectSUBMANDIBULAR-GLANDS
dc.subjectBUFFER CAPACITY
dc.subjectDENTAL-CARIES
dc.subjectFLOW-RATE
dc.subjectCA VI
dc.subjectMELLITUS
dc.subjectASSOCIATION
dc.titleThe investigation of genetic polymorphisms in the carbonic anhydrase VI gene exon 2 and salivary parameters in type 2 diabetic patients and healthy adults
dc.typearticle
dspace.entity.typePublication
local.avesis.id0b327911-d3d4-45d9-a57b-c68eeb1d6c63
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage5682
oaire.citation.issue5
oaire.citation.startPage5677
oaire.citation.titleMOLECULAR BIOLOGY REPORTS
oaire.citation.volume39
relation.isAuthorOfPublication46115086-6b46-4ef8-aaf1-ab8eda3e917e
relation.isAuthorOfPublication.latestForDiscovery46115086-6b46-4ef8-aaf1-ab8eda3e917e

Files

Collections

Research Outputs