Publication:
Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification

Simple item page
dc.contributor.authorŞİŞMAN, ALPER
dc.contributor.authorsHUSSEINI A. A., Yazdani A. M., Ghadiri F., ŞİŞMAN A.
dc.date.accessioned2023-12-11T08:16:23Z
dc.date.available2023-12-11T08:16:23Z
dc.date.issued2023-01-01
dc.description.abstractWe developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans cells and glass particles within the focal area. To optimize this SAW-induced streaming, we conducted 42 pilot experiments, varying electrical power, droplet volume, glass particle size, concentration, and lysis time, resulting in optimal conditions: an electrical signal of 2.5 W, a 20 μL sample volume, glass particle size below 10 μm, concentration of 0.2 μg, and a 5-min lysis period. We successfully amplified DNA target fragments directly from the lysate, demonstrating an efficient microchip-based cell lysis method. When combined with an isothermal amplification technique, this technology holds promise for rapid point-of-care (POC) applications.
dc.identifier.citationHUSSEINI A. A., Yazdani A. M., Ghadiri F., ŞİŞMAN A., "Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification", Engineering in Life Sciences, 2023
dc.identifier.doi10.1002/elsc.202300230
dc.identifier.issn1618-0240
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85178412744&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/295508
dc.language.isoeng
dc.relation.ispartofEngineering in Life Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiyomedikal Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectBiomedical Engineering
dc.subjectLife Sciences
dc.subjectBiotechnology
dc.subjectNatural Sciences
dc.subjectEngineering and Technology
dc.subjectMühendislik, Bilişim ve Teknoloji (ENG)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMühendislik
dc.subjectMikrobiyoloji
dc.subjectMÜHENDİSLİK, ÇEVRE
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectMÜHENDİSLİK, BİYOMEDİKAL
dc.subjectEngineering, Computing & Technology (ENG)
dc.subjectLife Sciences (LIFE)
dc.subjectENGINEERING
dc.subjectMICROBIOLOGY
dc.subjectENGINEERING, ENVIRONMENTAL
dc.subjectBIOTECHNOLOGY & APPLIED MICROBIOLOGY
dc.subjectENGINEERING, BIOMEDICAL
dc.subjectBiyomühendislik
dc.subjectFizik Bilimleri
dc.subjectÇevre Mühendisliği
dc.subjectBioengineering
dc.subjectPhysical Sciences
dc.subjectEnvironmental Engineering
dc.subjectcell lysis
dc.subjectdroplet
dc.subjectmicro-glass particle
dc.subjectsurface acoustic wave
dc.titleDeveloping a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification
dc.typearticle
dspace.entity.typePublication
local.avesis.ida991bce8-e029-42fc-83b0-95877f7af26c
local.indexed.atSCOPUS
relation.isAuthorOfPublication26706459-fa25-48f4-8e21-7b1560c9b9d7
relation.isAuthorOfPublication.latestForDiscovery26706459-fa25-48f4-8e21-7b1560c9b9d7

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
5.pdf
Size:
1.6 MB
Format:
Adobe Portable Document Format
Download

Collections

Research Outputs