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Sulfated levan from Halomonas smyrnensis as a bioactive, heparin-mimetic glycan for cardiac tissue engineering applications

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsErginer, Merve; Akcay, Ayca; Coskunkan, Binnaz; Morova, Tunc; Rende, Deniz; Bucak, Seyda; Baysal, Nihat; Ozisik, Rahmi; Eroglu, Mehmet S.; Agirbasli, Mehmet; Oner, Ebru Toksoy
dc.date.accessioned2022-03-12T20:29:53Z
dc.date.available2022-03-12T20:29:53Z
dc.date.issued2016
dc.description.abstractChemical derivatives of levan from Halomonas smyrnensis AAD6T with low, medium and high levels of sulfation were synthesized and characterized by FTIR and 2D-NMR. Sulfated levan samples were found to exhibit anticoagulation activity via the intrinsic pathway like heparin in a dose-dependent manner. Exceptionally high heparin equivalent activity of levan sulfate was shown to proceed via thrombin inhibition where decreased Factor Xa activity with increasing concentration was observed in antithrombin tests and above a certain concentration, levan sulfate showed a better inhibitor activity than heparin. In vitro experimental results were then verified in silico by docking studies using equilibrium structures obtained by molecular dynamic simulations and results suggested a sulfation dependent binding mechanism. With its high biocompatibility and heparin mimetic activity, levan sulfate can be considered as a suitable functional biomaterial to design biologically active, functionalized, thin films and engineered smart scaffolds for cardiac tissue engineering applications. (C) 2016 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.carbpol.2016.04.092
dc.identifier.eissn1879-1344
dc.identifier.issn0144-8617
dc.identifier.pubmed27261753
dc.identifier.urihttps://hdl.handle.net/11424/234128
dc.identifier.wosWOS:000377068100030
dc.language.isoeng
dc.publisherELSEVIER SCI LTD
dc.relation.ispartofCARBOHYDRATE POLYMERS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLevan
dc.subjectHalomonas smyrnensis
dc.subjectSulfation
dc.subjectHeparin mimetics
dc.subjectAnticoagulant activity
dc.subjectANTICOAGULANT ACTIVITY
dc.subjectANTIOXIDANT ACTIVITIES
dc.subjectPOLYSACCHARIDE
dc.subjectBIOMATERIALS
dc.subjectFILMS
dc.subjectSULFONATION
dc.subjectCOMPLEX
dc.titleSulfated levan from Halomonas smyrnensis as a bioactive, heparin-mimetic glycan for cardiac tissue engineering applications
dc.typearticle
dspace.entity.typePublication
local.avesis.idc5b436a8-bda9-48d6-9be1-effad6b7c9b0
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
local.journal.quartileQ1
oaire.citation.endPage296
oaire.citation.startPage289
oaire.citation.titleCARBOHYDRATE POLYMERS
oaire.citation.volume149
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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