Publication:
Comparison of VEGF gene silencing efficiencies of chitosan and protamine complexes containing shRNA

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dc.contributor.authorÖZBAŞ, SUNA
dc.contributor.authorsErdem-Cakmak, Fulden; Ozbas-Turan, Suna; Salva, Emine; Akbuga, Julide
dc.date.accessioned2022-03-13T12:45:32Z
dc.date.available2022-03-13T12:45:32Z
dc.date.issued2014
dc.description.abstractVEGF is an angiogenic factor promoting the proliferation and migration of endothelial cells. Inhibition of VEGF by RNAi mechanism is one of the novel and the most important strategies in antiangiogenesis therapy. In this study, the tumor silencing efficiency of ternary complexes after addition of protamine to chitosan complexes containing VEGF targeting shRNA was investigated. Besides chitosan, protamine is an effective gene delivery material. Binary and ternary complexes consisting of chitosan, protamine, and shRNA were prepared to target VEGF, their morphology, size, and zeta potential of the complexes being measured. The average size of the complexes was between 173 and 284nm and zeta potential was between +10 and 16mV. In the ternary complexes, size decreased as the chitosan ratio increased; however, its molecular weight had no effect on the size of complexes. HeLa, HEK293, and MCF-7 cell lines were used for in vitro transfection. VEGF was assayed by ELISA. A higher silencing effect was obtained using ternary complexes. Transgene expression was increased by adding protamine to chitosan complexes. Gene inhibition values in cell lines followed the rank HEK293>HeLa>MCF-7. The addition of protamine to the chitosan/shRNA (VEGF) complexes increased the knockdown of VEGF genes in the cell lines, and no cytotoxicity was found after the complexes had been incorporated into the cells.
dc.identifier.doi10.1002/cbin.10317
dc.identifier.eissn1095-8355
dc.identifier.issn1065-6995
dc.identifier.pubmed24890139
dc.identifier.urihttps://hdl.handle.net/11424/237800
dc.identifier.wosWOS:000342798200005
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofCELL BIOLOGY INTERNATIONAL
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectshort hairpin RNA
dc.subjectVEGF
dc.subjectchitosan
dc.subjectprotamine
dc.subjectcomplex
dc.subjectMOLECULAR-WEIGHT
dc.subjectDELIVERY
dc.subjectSIRNA
dc.subjectDNA
dc.subjectNANOPARTICLES
dc.subjectTRANSFECTION
dc.subjectNANOPLEXES
dc.subjectCANCER
dc.subjectTUMOR
dc.subjectANGIOGENESIS
dc.titleComparison of VEGF gene silencing efficiencies of chitosan and protamine complexes containing shRNA
dc.typearticle
dspace.entity.typePublication
local.avesis.idc083f179-714b-46ec-8c44-be0cb0ddfe8c
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages11
oaire.citation.endPage1270
oaire.citation.issue11
oaire.citation.startPage1260
oaire.citation.titleCELL BIOLOGY INTERNATIONAL
oaire.citation.volume38
relation.isAuthorOfPublicationd56d5fb1-37ec-4be6-8156-b58d67614019
relation.isAuthorOfPublication.latestForDiscoveryd56d5fb1-37ec-4be6-8156-b58d67614019

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