Publication:
Flow cytometry analysis of gingival and periodontal ligament cells

Simple item page
dc.contributor.authorKURU, LEYLA
dc.contributor.authorsKuru, L; Parkar, MH; Griffiths, GS; Newman, HN; Olsen, L
dc.date.accessioned2022-03-12T16:57:13Z
dc.date.available2022-03-12T16:57:13Z
dc.date.issued1998
dc.description.abstractGingival and periodontal ligament (PDL) fibroblasts are the major cellular components of periodontal soft connective tissues, but the precise differences between these cells are not yet known. In the present study, we have therefore examined the phenotypic and functional features of the cells obtained from gingival and PDL biopsy samples. Spindle-shaped cells characteristic of fibroblasts were the main cell type observed in vitro, although epithelial cells were also present in primary gingival cell cultures. Flow cytometry was used to measure the size and granularity of the cultured cells, and showed that the gingival fibroblasts were smaller and less granular compared with the PDL cells. The expression of certain key extracellular matrix (ECM) proteins, fibronectin, collagen type I, and tenascin was measured by flow cytometry. Analysis of the fluorescence profiles of these cultures showed that the majority of cells expressed fibronectin and that the average fluorescence intensity of this antigen in the PDL cells was higher than that in the gingival fibroblasts. Moreover, the fibronectin-positive PDL cells apparently comprised two subpopulations which expressed fibronectin at different levels, suggesting that the cells in the PDL cultures were functionally heterogeneous. The level of collagen type I was also found to be upregulated in the PDL compared with the gingival cells and, as with fibronectin, was expressed at two different levels by subsets of the PDL cells. In contrast, tenascin was expressed at very similar levels by both the gingival fibroblasts and PDL cells. In addition, measurement of alkaline phosphatase, a marker enzyme for mineralized tissue-forming cells, showed that the PDL cells had higher activity than the gingival fibroblasts and that the alkaline phosphatase activity in the PDL cells was far more markedly up-regulated by dexamethasone. Our findings demonstrate that, despite their similar spindle-shaped appearance, fibroblasts derived from gingival and PDL tissues appear to display distinct functional activities which are likely to play a vital part in the maintenance of tissue integrity and regenerative processes.
dc.identifier.doi10.1177/00220345980770040801
dc.identifier.issn0022-0345
dc.identifier.pubmed9539458
dc.identifier.urihttps://hdl.handle.net/11424/226901
dc.identifier.wosWOS:000072803900008
dc.language.isoeng
dc.publisherAMER ASSOC DENTAL RESEARCH
dc.relation.ispartofJOURNAL OF DENTAL RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectfibroblast
dc.subjectgingiva
dc.subjectperiodontal ligament
dc.subjectheterogeneity
dc.subjectflow cytometry
dc.subjectHUMAN PERMANENT TEETH
dc.subjectFIBROBLAST SUBPOPULATIONS
dc.subjectIMMUNOHISTOCHEMICAL LOCALIZATION
dc.subjectALKALINE-PHOSPHATASE
dc.subjectATTACHMENT FORMATION
dc.subjectTISSUE REGENERATION
dc.subjectIN-VITRO
dc.subjectTENASCIN
dc.subjectINVITRO
dc.subjectEXPRESSION
dc.titleFlow cytometry analysis of gingival and periodontal ligament cells
dc.typearticle
dspace.entity.typePublication
local.avesis.id7879ac7c-3028-4db3-b986-9dc538ff7810
local.import.packageSS17
local.indexed.atWOS
local.indexed.atPUBMED
local.journal.numberofpages10
oaire.citation.endPage564
oaire.citation.issue4
oaire.citation.startPage555
oaire.citation.titleJOURNAL OF DENTAL RESEARCH
oaire.citation.volume77
relation.isAuthorOfPublicationcc49fe59-fb00-4a6a-a32c-60d68c5f3993
relation.isAuthorOfPublication.latestForDiscoverycc49fe59-fb00-4a6a-a32c-60d68c5f3993

Files

Collections

Research Outputs