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How prenatal environmental factors affect rat molar enamel formation

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dc.contributor.authorÖZKAN YENAL, NAZİYE
dc.contributor.authorMENTEŞ, ALİ RECAİ
dc.contributor.authorsDuman C., ÖZKAN YENAL N., MENTEŞ A. R.
dc.date.accessioned2023-03-27T09:58:04Z
dc.date.available2023-03-27T09:58:04Z
dc.date.issued2022-10-01
dc.description.abstractAmelogenin (AMELX) and ameloblastin (AMBN) are crucial for enamel formation, and interruptions in the production of these proteins may cause enamel defects. We investigated how prenatal environmental factors (chronic stress, bisphenol A (BPA), amoxicillin, and lipopolysaccharide (LPS)) affect AMELX and AMBN production of ameloblasts. Fifteen pregnant Sprague-Dawley rats were divided into four experimental groups and a control group. Chronic-stress group rats were exposed to a 12:12 light/light cycle (LL) from day E18 until delivery. BPA group rats were orally administered 5 mu g/kg BPA daily from day E1 until delivery. Amoxicillin group rats were injected 100 mg/kg amoxicillin daily from day E18 until delivery. LPS-infection group rats were injected 125 mu g/kg bacterial LPS once on day E18. Seven pups from the control group and ten pups from the experimental groups were euthanized on P10. Sections were stained with hematoxylin and eosin (H&E) and Gomori\"s one-step trichrome staining (GT) and incubated with rabbit polyclonal antibodies to AMELX and AMBN, to evaluate staining intensity at ameloblast stages. The surface morphology was evaluated with a stereomicroscope. AMELX (p = 0.008, p = 0.0001, p = 0.009) and AMBN (p = 0.002, p = 0.001, p = 0.0001) staining of all groups were significantly lower than that of the control group in the secretory, transitional, and maturation stages. Abnormal enamel matrix formation was observed in the H&E and GT staining sections of all experimental groups. Yellowish coloration of the amoxicillin group was observed in morphologic evaluation.
dc.identifier.citationDuman C., ÖZKAN YENAL N., MENTEŞ A. R., "How prenatal environmental factors affect rat molar enamel formation?", ODONTOLOGY, cilt.110, sa.4, ss.655-663, 2022
dc.identifier.doi10.1007/s10266-022-00699-4
dc.identifier.endpage663
dc.identifier.issn1618-1247
dc.identifier.issue4
dc.identifier.startpage655
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/35262822/
dc.identifier.urihttps://hdl.handle.net/11424/287890
dc.identifier.volume110
dc.language.isoeng
dc.relation.ispartofODONTOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectDiş Hekimliği
dc.subjectMedicine
dc.subjectHealth Sciences
dc.subjectDentistry
dc.subjectDİŞ HEKİMLİĞİ, ORAL CERRAHİ VE TIP
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectDENTISTRY, ORAL SURGERY & MEDICINE
dc.subjectCLINICAL MEDICINE
dc.subjectClinical Medicine (MED)
dc.subjectOrthodontics
dc.subjectOral Surgery
dc.subjectDentistry (miscellaneous)
dc.subjectDental Hygiene
dc.subjectPeriodontics
dc.subjectDental Assisting
dc.subjectGeneral Dentistry
dc.subjectEnamel
dc.subjectDevelopmental biology
dc.subjectEnamel formation
dc.subjectExtracellular matrix
dc.subjectHistochemistry
dc.subjectSTRESS
dc.subjectAMOXICILLIN
dc.subjectEXPOSURE
dc.subjectINCISOR
dc.subjectAMELOBLASTIN
dc.subjectPREVALENCE
dc.subjectETIOLOGY
dc.subjectDEFECTS
dc.subjectMATRIX
dc.subjectGROWTH
dc.subjectEnamel
dc.subjectDevelopmental biology
dc.subjectEnamel formation
dc.subjectExtracellular matrix
dc.subjectHistochemistry
dc.titleHow prenatal environmental factors affect rat molar enamel formation
dc.typearticle
dspace.entity.typePublication
local.avesis.idbf03ab2d-5cbd-4ae0-901b-2460721270d7
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
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relation.isAuthorOfPublication.latestForDiscoverya5fed550-3bee-48f8-afb1-5cc073b0af9d

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