Publication:
Lack of Serotype Antigen in A. actinomycetemcomitans

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dc.contributor.authorDOĞAN, BAŞAK
dc.contributor.authorsKanasi, E.; Dogan, B.; Karched, M.; Thay, B.; Oscarsson, J.; Asikainen, S.
dc.date.accessioned2022-03-12T17:47:50Z
dc.date.available2022-03-12T17:47:50Z
dc.date.issued2010
dc.description.abstractAggregatibacter actinomycetemcomitans is divided into 6 serotypes. Occurrence of non-serotypeable strains is known, but background reasons are unclear. We hypothesized that non-serotypeable strains represent new serotypes or have altered expression of serotype-specific polysaccharide antigen (S-PA). We first characterized 311 strains from 189 individuals using both immunoassay-and PCR-based serotyping. Next, using natural human infection and rabbit immunization approaches, we clarified whether the phenotypically non-serotypeable strains expressed S-PA. Immunoassay identified serotypes a-f among 216 strains from 159 individuals. The remaining 95 strains from 30 individuals were phenotypically non-serotypeable. Yet, all these strains were identified by PCR-typing as serotype a-, b-, c-, or f. Non-serotypeability was confirmed by Western immunoblot with respective rabbit antisera. Patient sera remained non-reactive with autologous non-serotypeable strains at the serotype-specific region. Rabbit immunization with a phenotypically non-serotypeable strain induced no antibody production against S-PA. Thus, phenotypically non-serotypeable strains did not include novel serotypes, but lacked S-PA expression.
dc.identifier.doi10.1177/0022034509358865
dc.identifier.eissn1544-0591
dc.identifier.issn0022-0345
dc.identifier.pubmed20139341
dc.identifier.urihttps://hdl.handle.net/11424/229845
dc.identifier.wosWOS:000274431100014
dc.language.isoeng
dc.publisherSAGE PUBLICATIONS INC
dc.relation.ispartofJOURNAL OF DENTAL RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectA. actinomycetemcomitans
dc.subjectserotype antigen
dc.subjectnon-serotypeability
dc.subjectperiodontitis
dc.subjectgene expression
dc.subjectHUMAN PERIODONTAL-DISEASE
dc.subjectACTINOBACILLUS-ACTINOMYCETEMCOMITANS
dc.subjectAGGREGATIBACTER-ACTINOMYCETEMCOMITANS
dc.subjectPORPHYROMONAS-GINGIVALIS
dc.subjectJUVENILE PERIODONTITIS
dc.subjectO-POLYSACCHARIDE
dc.subjectLIPOPOLYSACCHARIDE
dc.subjectSTRAINS
dc.subjectCOAGGREGATION
dc.subjectPOPULATION
dc.titleLack of Serotype Antigen in A. actinomycetemcomitans
dc.typearticle
dspace.entity.typePublication
local.avesis.idbb16a9b4-cef6-4a4a-b418-1fe1db64e29d
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages5
oaire.citation.endPage296
oaire.citation.issue3
oaire.citation.startPage292
oaire.citation.titleJOURNAL OF DENTAL RESEARCH
oaire.citation.volume89
relation.isAuthorOfPublicationa2569f78-7a2a-4f98-8cc9-d8b67ae56f6d
relation.isAuthorOfPublication.latestForDiscoverya2569f78-7a2a-4f98-8cc9-d8b67ae56f6d

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