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Performance of RESIST-3 OKN K-SeT immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey

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dc.contributor.authorALTINKANAT GELMEZ, GÜLŞEN
dc.contributor.authorsSagiroglu, Pinar; Hasdemir, Ufuk; Gelmez, Gulsen Altinkanat; Aksu, Burak; Karatuna, Onur; Soyletir, Guner
dc.date.accessioned2022-03-12T22:26:30Z
dc.date.available2022-03-12T22:26:30Z
dc.date.issued2018
dc.description.abstractIn this study, the performance of the RESIST-3 O.K.N. K-SeT (Cofis BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla(KPC), bla(VIM) bla(NDM), and bla(OXA-48). The rates of bla(NDM), bla(OXA-48), and bla(KPC) in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla(NDM) and bla(OXA-48) were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132 K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying bla(KPC), bla(NDM), and/or bla(OXA-48). On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring bla(IMP) and bla(VIM). We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
dc.identifier.doi10.1016/j.bjm.2018.002
dc.identifier.eissn1678-4405
dc.identifier.issn1517-8382
dc.identifier.pubmed29548716
dc.identifier.urihttps://hdl.handle.net/11424/235074
dc.identifier.wosWOS:000447893000026
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofBRAZILIAN JOURNAL OF MICROBIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectKlebsiella pneumoniae
dc.subjectImmunochromatographic test
dc.subjectNDM
dc.subjectKPC
dc.subjectOXA-48
dc.subjectRAPID DETECTION
dc.subjectESCHERICHIA-COLI
dc.subjectPSEUDOMONAS-AERUGINOSA
dc.subject1ST REPORT
dc.subjectENTEROBACTERIACEAE
dc.subjectIDENTIFICATION
dc.subjectISTANBUL
dc.subjectSTRAINS
dc.subjectOXA-181
dc.subjectISOLATE
dc.titlePerformance of RESIST-3 OKN K-SeT immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey
dc.typearticle
dspace.entity.typePublication
local.avesis.idf4d772e1-6043-450d-adaa-e22732afc3ca
local.import.packageSS17
local.indexed.atWOS
local.journal.numberofpages6
local.journal.quartileQ2
oaire.citation.endPage890
oaire.citation.issue4
oaire.citation.startPage885
oaire.citation.titleBRAZILIAN JOURNAL OF MICROBIOLOGY
oaire.citation.volume49
relation.isAuthorOfPublication49c5539e-4c6e-4588-b52c-dcc5cb2979ab
relation.isAuthorOfPublication.latestForDiscovery49c5539e-4c6e-4588-b52c-dcc5cb2979ab

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