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Simple and reliable detection of slime production of Candida spp. directly from blood culture bottles: Comparison of visual tube method and transmission electron microscopy

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dc.contributor.authorHASDEMİR GÖKBOĞA, MÜNEVVER UFUK
dc.contributor.authorsCerikcioglu, N; Hasdemir, UO; San, T; Salik, E; Soyletir, G
dc.date.accessioned2022-03-12T17:16:28Z
dc.date.available2022-03-12T17:16:28Z
dc.date.issued2004
dc.description.abstractEarly detection of slime production may be useful for clinical decision because of its suggestive property for potential pathogenic capacity of a Candida strain especially in patients with a prosthetic device. In this study we aimed to compare the visual tube method (VTM) with transmission electron microscopy (TEM) in order to confirm the reliability of the former method. In order to demonstrate the reproducibility of the tube method and to determine the correct timing for the test, Candida isolates directly obtained from blood culture (DBC) bottles and their two subsequent subcultures were used. The results of this study showed that VTM is a simple and reliable method which can be used in every clinical mycology laboratory, provided that the test is applied on DBC isolates; as the ability of slime production is decreased or lost even after the first subculturing. We suggest that this simple method can be used and may have some contributions to the ongoing studies on the controversial issue concerning removal of biomaterials in candidemic patients.
dc.identifier.doi10.1007/s11046-004-0927-4
dc.identifier.eissn1573-0832
dc.identifier.issn0301-486X
dc.identifier.pubmed15645168
dc.identifier.urihttps://hdl.handle.net/11424/227583
dc.identifier.wosWOS:000226417300003
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofMYCOPATHOLOGIA
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectblood culture
dc.subjectCandida spp.
dc.subjectslime production
dc.subjectCOAGULASE-NEGATIVE STAPHYLOCOCCI
dc.subjectBIOFILM FORMATION
dc.subjectMICROBIAL BIOFILMS
dc.subjectBACTERIAL BIOFILMS
dc.subjectVASCULAR CATHETERS
dc.subjectANTIFUNGAL AGENTS
dc.subjectACRYLIC SURFACES
dc.subjectIN-VITRO
dc.subjectALBICANS
dc.subjectPARAPSILOSIS
dc.titleSimple and reliable detection of slime production of Candida spp. directly from blood culture bottles: Comparison of visual tube method and transmission electron microscopy
dc.typearticle
dspace.entity.typePublication
local.avesis.idb32952f1-89ed-4510-996c-90358c2caf92
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage284
oaire.citation.issue3
oaire.citation.startPage279
oaire.citation.titleMYCOPATHOLOGIA
oaire.citation.volume158
relation.isAuthorOfPublicationc6059919-1fb4-499b-84ad-9afa4bb224d8
relation.isAuthorOfPublication.latestForDiscoveryc6059919-1fb4-499b-84ad-9afa4bb224d8

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