Publication:
Antiproliferative activity of whey and casein bioactive peptides on breast cancer: an in vitro and in silico study

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dc.contributor.authorAKBAY, TUĞBA
dc.contributor.authorsSahna K. O., Cakir B., Tunali-Akbay T.
dc.date.accessioned2023-07-17T09:00:26Z
dc.date.available2023-07-17T09:00:26Z
dc.date.issued2022-07-01
dc.description.abstractBioactive peptides from milk proteins are dietary components with various biological properties and therapeutic effects in a variety of health conditions, including cancer. In this study, the effects of goat milk proteins and bioactive peptides on the MCF-7 breast cancer cell line were investigated. Milk was separated into casein and Whey fractions and treated with either pepsin or trypsin enzymes. Peptides of the hydrolyzed fractions were identified with LC-QTOFF/MS. After identification, the hydrolyzed Whey and casein fractions were incubated with MCF-7 breast cancer cells at various concentrations. The in vitro cytotoxicity of hydrolyzed fractions against MCF-7 cells was tested using MTT assay and flow cytometry analysis. The dead MCF-7 cells were analyzed with LC-Q-TOF/MS. In dead MCF-7 cells, the pyruvate kinase M2, mucin 1-C, glycogen synthase kinase 3-beta, and L-lactate dehydrogenase B were found to be downregulated in the LC-Q-TOF/MS analysis, while receptor-interacting serine/ threonine-protein kinase 1 was found to be up-regulated. These enzymes and mucin-1C also interacted with the milk-derived bioactive peptides in silico. The goat milk bioactive peptides exhibited high binding affinity with these enzymes and mucin-1C. The 5 mu g mL(-1) trypsin-treated casein fraction caused the highest MCF-7 cell death. In conclusion, the bioactive peptides of the pepsin-treated casein fraction and trypsin-treated Whey fraction of goat milk may inhibit the survival of breast cancer cells and exert their effects via multiple mechanisms. The results of this study are novel, suggesting that goat milk-based specific bioactive peptides may have potential as an anti-tumour agent in the treatment of breast cancer.
dc.identifier.citationSahna K. O., Cakir B., Tunali-Akbay T., "Antiproliferative Activity of Whey and Casein Bioactive Peptides on Breast Cancer: An In Vitro and In Silico Study", INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, cilt.28, sa.4, 2022
dc.identifier.doi10.1007/s10989-022-10436-8
dc.identifier.issn1573-3149
dc.identifier.issue4
dc.identifier.urihttps://link.springer.com/article/10.1007/s10989-022-10436-8
dc.identifier.urihttps://hdl.handle.net/11424/291318
dc.identifier.volume28
dc.language.isoeng
dc.relation.ispartofINTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectCytogenetic
dc.subjectNatural Sciences
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBIOCHEMISTRY & MOLECULAR BIOLOGY
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectLife Sciences (LIFE)
dc.subjectBiyokimya, Genetik ve Moleküler Biyoloji (çeşitli)
dc.subjectKlinik Biyokimya
dc.subjectKanser Araştırmaları
dc.subjectMoleküler Biyoloji
dc.subjectİlaç Keşfi
dc.subjectYaşlanma
dc.subjectGenel Biyokimya, Genetik ve Moleküler Biyoloji
dc.subjectBiyokimya
dc.subjectYapısal Biyoloji
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectClinical Biochemistry
dc.subjectCancer Research
dc.subjectMolecular Biology
dc.subjectDrug Discovery
dc.subjectAging
dc.subjectGeneral Biochemistry, Genetics and Molecular Biology
dc.subjectBiochemistry
dc.subjectStructural Biology
dc.subjectGoat milk
dc.subjectCasein
dc.subjectWhey proteins
dc.subjectBioactive peptides
dc.subjectBreast cancer
dc.subjectGOAT MILK
dc.subjectTUMOR
dc.subjectAPOPTOSIS
dc.subjectCYTOMETRY
dc.subjectARGININE
dc.subjectSURFACE
dc.subjectLYSINE
dc.subjectRICH
dc.titleAntiproliferative activity of whey and casein bioactive peptides on breast cancer: an in vitro and in silico study
dc.typearticle
dspace.entity.typePublication
local.avesis.idb03aadaf-553f-4ca3-929d-0adf59be88ed
local.indexed.atWOS
local.indexed.atSCOPUS
relation.isAuthorOfPublication340aca72-e793-4abe-b0d6-2af5ca6013e3
relation.isAuthorOfPublication.latestForDiscovery340aca72-e793-4abe-b0d6-2af5ca6013e3

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