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The effect of whey proteins on the brain and small intestine nitric oxide levels: protein profiles in methotrexate-induced oxidative stress

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dc.contributor.authorÖZBEYLİ, DİLEK
dc.contributor.authorAKBAY, TUĞBA
dc.contributor.authorsYILMAZ KARAOĞLU S., Tufan E., Sivas G. G., Gürel Gökmen B., Dursun E., ÖZBEYLİ D., ŞENER G., AKBAY T.
dc.date.accessioned2023-01-10T10:36:23Z
dc.date.available2023-01-10T10:36:23Z
dc.date.issued2022-12-01
dc.description.abstractObjectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-Stransferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain’s NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage
dc.identifier.citationYILMAZ KARAOĞLU S., Tufan E., Sivas G. G., Gürel Gökmen B., Dursun E., ÖZBEYLİ D., ŞENER G., AKBAY T., "The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress", Istanbul University, cilt.12, 2022
dc.identifier.doi10.26650/experimed.1189898
dc.identifier.endpage118
dc.identifier.issn2667-5846
dc.identifier.startpage113
dc.identifier.urihttp://dx.doi.org/10.26650/experimed.1189898
dc.identifier.urihttps://hdl.handle.net/11424/285055
dc.identifier.volume12
dc.language.isoeng
dc.relation.ispartofIstanbul University
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMethotrexate
dc.subjectwhey protein
dc.subjectbrain
dc.subjectsmall intestine
dc.subjectnitric oxide
dc.subjectoxidative stress
dc.titleThe effect of whey proteins on the brain and small intestine nitric oxide levels: protein profiles in methotrexate-induced oxidative stress
dc.typearticle
dspace.entity.typePublication
local.avesis.id79cfee90-9270-45c1-b9c1-72a079b2c0c1
relation.isAuthorOfPublication4681bb67-e40b-4cf0-8154-5472237f2325
relation.isAuthorOfPublication340aca72-e793-4abe-b0d6-2af5ca6013e3
relation.isAuthorOfPublication.latestForDiscovery4681bb67-e40b-4cf0-8154-5472237f2325

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