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Spectrophotometric determination of leukocytes in urine

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorsImren-Eryilmaz, E; Kuzu-Karsilayan, H; Ogan, A
dc.date.accessioned2022-03-14T09:53:18Z
dc.date.available2022-03-14T09:53:18Z
dc.date.issued2004
dc.description.abstractA spectrophotometric method based on myeloperoxidase activity for the determination of leukocytes in urine is described. Red cells that may be found in urine samples were lysed by an ammonium chloride method. Leukocytes were then sedimented by centrifugation and lysed using Triton X-100 (Sigma Chemicals Co., St. Louis, MO). Myeloperoxidase-catalyzed oxidation of o-dianisidine was carried out at 37degreesC, pH 7. The reaction was stopped with the addition of 2 M H2SO4, and a stable form of oxidized o-dianisidine in acidic solution was obtained. Solid particles that may be found in urine samples were removed by centrifugation to avoid turbidity, and absorbance values of the supernatants were recorded at 400 rim. An Average number of leukocytes were noted per number of fields by microscopic examination and were related with the absorbance values of the supernatants at 400 nm. Pearson correlation (r) between our presented spectrophotometric analysis results and visual microscopic analysis was 0.877. Roche Combur 10-test M strips (Roche, Mannheim, Germany) and Multistix 10 SG Bayer test strips (Bayer Diagnostics, UK) were 0.645 and 0.648, respectively (P < 0.0001). (C) 2004 Wiley-Liss, Inc.
dc.identifier.doi10.1002/jcla.20032
dc.identifier.eissn1098-2825
dc.identifier.issn0887-8013
dc.identifier.pubmed15202119
dc.identifier.urihttps://hdl.handle.net/11424/243547
dc.identifier.wosWOS:000222485400007
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF CLINICAL LABORATORY ANALYSIS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectleukocyte
dc.subjectdetermination
dc.subjecturine
dc.subjectspectrophotometry
dc.subjecto-dianisidine
dc.subject5-LIPOXYGENASE ACTIVITY
dc.subjectEOSINOPHIL PEROXIDASE
dc.subjectHUMAN-NEUTROPHILS
dc.subjectSINGLET OXYGEN
dc.subjectTEST STRIPS
dc.subjectMYELOPEROXIDASE
dc.subjectURINALYSIS
dc.subjectOXIDATION
dc.subjectCHLORIDE
dc.subjectBROMIDE
dc.titleSpectrophotometric determination of leukocytes in urine
dc.typearticle
dspace.entity.typePublication
local.avesis.idc8fd02d8-9b46-4374-9227-3b6c24f389c9
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages4
oaire.citation.endPage254
oaire.citation.issue4
oaire.citation.startPage251
oaire.citation.titleJOURNAL OF CLINICAL LABORATORY ANALYSIS
oaire.citation.volume18
relation.isAuthorOfPublication58db3332-a5f8-4224-a021-aeea795f52fa
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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