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The effect of losartan and captopril on glomerular basement membrane anionic charge in a diabetic rat model

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dc.contributor.authorYAVUZ, DİLEK
dc.contributor.authorsYavuz, DG; Ersoz, O; Kucukkaya, B; Budak, Y; Ahiskali, R; Ekicioglu, G; Emerk, K; Akalin, S
dc.date.accessioned2022-03-12T16:59:43Z
dc.date.available2022-03-12T16:59:43Z
dc.date.issued1999
dc.description.abstractObjective Although angiotensin-converting enzyme inhibitors are known to reduce albuminuria by preserving glomerular basement membrane anionic content, the effects of angiotensin II receptor blockage are currently not known. The aim of this study was to evaluate the effects of captopril and losartan on glomerular basement membrane anionic charges in a diabetic rat model. Design After diabetes induction with streptozotocin, female Wistar rats were divided into three groups: group A, losartan 10 mg/kg by gavage (n = 8); group B, captopril 50 mg/l in drinking water (n = 6); group C, diabetic control rats (n = 8) given only tap water. Group D (eight rats) served as non-diabetic controls. At the end of 8 weeks, erythrocyte membrane charge, serum sialic acid, urinary glycosaminoglycan and albumin were measured and kidney specimens stained with Alcian blue in order to assess basement membrane glycosaminoglycans. Results Red blood cell anionic charges (ng Alcian blue/10(6) red blood cells) were 371.5 +/- 9.9 for group A, 443.5 +/- 7.1 for group B, 400.1 +/- 14.7 for group C, 468.7 +/- 4 for group D (A < D, C < D, P < 0.01). Serum sialic acid levels (mg/dl) were 90.6 +/- 14.1 for group A, 45.6 +/- 6.8 for group B, 89.1 +/- 8.5 for group C, 50.8 +/- 6.4 for group D (A, C > D, P < 0.01; A > B P < 0.01). Albuminuria (mu g/day) was 778 +/- 221 for group A, 719 +/- 314 for group B, 1724 +/- 945 for group C, 393 +/- 263 for group D (A, B < C, P < 0.05). Urinary glycosaminoglycan/creatinine ratio was 14.2 +/- 1.1 for group A, 9.9 +/- 1 for group B; 28.3 +/- 8 for group C, 5.5 +/- 1.7 for group D (B < C, P < 0.001; B < A, P < 0.003). Alcian blue staining was 1.8 +/- 0.2, 2.2 +/- 0.4, 1.5 +/- 0.16, 2.8 +/- 0.2 for groups A, B, C and D respectively (C, A < D P < 0.05). Conclusion Losartan and captopril have comparable effects on reducing albuminuria in a diabetic rat model. While captopril preserves basement membrane anionic charges, losartan has no effect. The anti-proteinuric effects of these drugs seem to have different mechanisms. I Hypertens 1999, 17:1217-1223 (C) Lippincott Williams & Wilkins.
dc.identifier.doi10.1097/00004872-199917080-00023
dc.identifier.issn0263-6352
dc.identifier.pubmed10466479
dc.identifier.urihttps://hdl.handle.net/11424/227230
dc.identifier.wosWOS:000081964600023
dc.language.isoeng
dc.publisherLIPPINCOTT WILLIAMS & WILKINS
dc.relation.ispartofJOURNAL OF HYPERTENSION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalbuminuria
dc.subjectcaptopril
dc.subjectdiabetes
dc.subjecterythrocyte membrane charge
dc.subjectglomerular anionic charge
dc.subjectglycosaminoglycan
dc.subjectlosartan
dc.subjectrat
dc.subjectSERUM SIALIC-ACID
dc.subjectCONVERTING ENZYME-INHIBITION
dc.subjectANGIOTENSIN-II
dc.subjectSIZE-SELECTIVITY
dc.subjectBLOOD-PRESSURE
dc.subjectMICROALBUMINURIA
dc.subjectNEPHROPATHY
dc.subjectMELLITUS
dc.subjectALBUMINURIA
dc.subjectENALAPRIL
dc.titleThe effect of losartan and captopril on glomerular basement membrane anionic charge in a diabetic rat model
dc.typearticle
dspace.entity.typePublication
local.avesis.id726f90b5-aec8-45a8-9cbc-d5855b5cea2f
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage1223
oaire.citation.issue8
oaire.citation.startPage1217
oaire.citation.titleJOURNAL OF HYPERTENSION
oaire.citation.volume17
relation.isAuthorOfPublication26174ec5-7dca-4038-a7fe-9a43f236fd15
relation.isAuthorOfPublication.latestForDiscovery26174ec5-7dca-4038-a7fe-9a43f236fd15

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