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Effect of Dental Follicle Mesenchymal Stem Cells on Th1 and Th2 Derived Naive T Cells in Atopic Dermatitis Patients

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dc.contributor.authorGÖKER, MEHMET KAMİL
dc.contributor.authorsZibandeh, Noushin; Genc, Deniz; Ozgen, Zuleyha; Duran, Yazgul; Kasap, Nurhan; Goker, Kamil; Baris, Safa; Ergun, Tulin; Akkoc, Tunc
dc.date.accessioned2022-03-14T09:08:22Z
dc.date.available2022-03-14T09:08:22Z
dc.date.issued2019-08-31
dc.description.abstractObjective: The purpose of our study is to investigate the immunomodulatory effects of Dental Follicle Mesenchymal Stem Cells (DF-MSCs) on lymphocytes isolated from peripheral blood of Atopic Dermatitis (AD) patients, a Th2 disease and psoriasis, a Th1 / Th17 disease and compare them with healthy individuals in vitro. Methods: Patients with the AD (n = 9) and psoriasis (n = 6) who are followed up in Marmara University Pediatric Allergy and Immunology and Dermatology outpatient clinics and healthy subjects (n = 6) were included. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 20 ml of venous blood of all participants. Cells were cultured for 72 hours in the absence and presence of DF-MSCs with anti-CD3/anti-CD28 stimulation or without stimulation. At the end of this period, CD4+ and CD8+ T lymphocyte proliferation and cytokine levels from the culture supernatants were analyzed by flow cytometry. Results: In the presence of DF-MSCs, proliferation ratio was suppressed in both CD4+ and CD8+ cells in AD and psoriasis patients (p<0,05). IFN-gamma levels significantly increased in AD patients in the presence of DF-MSCs (p<0,05) whereas decreased significantly in psoriasis patients in the presence of DF-MSCs (p<0,05). IL-4 levels significantly decreased in AD patients in the presence of DF-MSCs (p<0,05) but remained unchanged in psoriasis patients (p>0,05). IL-10 increased significantly in both groups in the presence of DF-MSCs (p<0,05). Conclusion: Our results support immunoregulatory effects of DF-MSCs on both AD and psoriasis which are Th2 and Th1 / Th17 dominant diseases respectively. Our evidence-based results demonstrated that DF-MSCs could have a beneficial therapeutic implication for inflammatory skin diseases.
dc.identifier.doi10.33808/clinexphealthsci.600104
dc.identifier.issn2459-1459
dc.identifier.urihttps://hdl.handle.net/11424/242615
dc.identifier.wosWOS:000493400900004
dc.language.isoeng
dc.publisherMARMARA UNIV, INST HEALTH SCIENCES
dc.relation.ispartofCLINICAL AND EXPERIMENTAL HEALTH SCIENCES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMesenchymal Stem Cells
dc.subjectImmunoregulation
dc.subjectSoluble factors
dc.subjectAtopic Dermatitis
dc.subjectPsoriasis
dc.subjectINTERFERON-GAMMA
dc.subjectDISEASE
dc.titleEffect of Dental Follicle Mesenchymal Stem Cells on Th1 and Th2 Derived Naive T Cells in Atopic Dermatitis Patients
dc.typearticle
dspace.entity.typePublication
local.avesis.id2f3f1a07-6e1b-404a-8f44-1aab44055bee
local.import.packageSS16
local.indexed.atWOS
local.indexed.atTRDIZIN
local.journal.numberofpages8
oaire.citation.endPage227
oaire.citation.issue3
oaire.citation.startPage220
oaire.citation.titleCLINICAL AND EXPERIMENTAL HEALTH SCIENCES
oaire.citation.volume9
relation.isAuthorOfPublication3e89a804-9977-4275-b0f2-8269bbe2740c
relation.isAuthorOfPublication.latestForDiscovery3e89a804-9977-4275-b0f2-8269bbe2740c

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