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Protective Effect of Nicotine on Sepsis-Induced Oxidative Multiorgan Damage: Role of Neutrophils

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dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsOzdemir-Kumral, Zarife N.; Ozbeyli, Dilek; Ozdemir, Ahmet F.; Karaaslan, Bugra M.; Kaytaz, Kubra; Kara, Mustafa F.; Tok, Olgu E.; Ercan, Feriha; Yegen, Berrak C.
dc.date.accessioned2022-03-12T20:30:49Z
dc.date.available2022-03-12T20:30:49Z
dc.date.issued2017
dc.description.abstractDespite its adverse health consequences, tobacco smoking is associated with lower incidence of several neurodegenerative and inflammatory diseases. The present study is aimed to show the effects of nicotine, major tobacco constituent, on five organs targeted by sepsis. Male Wistar albino rats received tap water with (5mg/kg) or without nicotine for 14 days. Under ketamine anesthesia, sepsis (n = 50) was induced by ligation and puncture of the cecum, while sham group (n = 8) had only laparotomy. In other rats, nicotine drink was withdrawn for 5 days before sepsis induction, while in acute nicotine group, rats were injected with nicotine (30mg/kg, i.p.) before sepsis, but had no oral intake. Rats were decapitated 24 hours after surgery to obtain lung, liver, ileum, heart, and kidney tissues to determine malondialdehyde (MDA) and glutathione (GSH) levels and myeloperoxidase (MPO) activities. Data were analyzed by one-way analysis of variance and Tukey multiple comparison tests or Student's t test. Chronic nicotine administration or its withdrawal reduced lipid peroxidation and MPO activity and prevented GSH depletion with some varying results in different target tissues. Nicotine injection prior to sepsis depressed MPO activity in all tissues and reduced MDA levels except for the lung, while GSH levels were elevated only in the hepatic and ileal tissues. Histologically observed injury was ameliorated by all nicotine treatments at varying degrees. The findings of the present study indicate that long-term nicotine administration reduces sepsis-induced oxidative damage in several tissues, which appears to involve inhibition of neutrophil activity in the inflamed tissues. Nicotine administration or its withdrawal reduced lipid peroxidation and neutrophil content and prevented GSH depletion with some varying results in different target tissues. A single injection prior to sepsis induction depressed MPO activity in all the tissues and reduced all tissue MDA levels except for the lung. When nicotine was withdrawn for 5 days, its inhibitory effect on MPO activity was still present in all the tissues except for the liver. Microscopically an improved inflammatory response was observed in all the tissues of rats that have received different nicotine pretreatment regimens.
dc.identifier.doi10.1093/ntr/ntw198
dc.identifier.eissn1469-994X
dc.identifier.issn1462-2203
dc.identifier.pubmed27613897
dc.identifier.urihttps://hdl.handle.net/11424/234216
dc.identifier.wosWOS:000404930300013
dc.language.isoeng
dc.publisherOXFORD UNIV PRESS
dc.relation.ispartofNICOTINE & TOBACCO RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectORGAN INJURY
dc.subjectRAT MODEL
dc.subjectSMOKING
dc.subjectINFLAMMATION
dc.subjectEXPOSURE
dc.subjectACTIVATION
dc.subjectMORTALITY
dc.subjectSURVIVAL
dc.subjectCOLITIS
dc.subjectDISEASE
dc.titleProtective Effect of Nicotine on Sepsis-Induced Oxidative Multiorgan Damage: Role of Neutrophils
dc.typearticle
dspace.entity.typePublication
local.avesis.id8349b166-61cd-44a1-9068-bf4926696a0a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
local.journal.quartileQ1
oaire.citation.endPage864
oaire.citation.issue7
oaire.citation.startPage859
oaire.citation.titleNICOTINE & TOBACCO RESEARCH
oaire.citation.volume19
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

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