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Metagenomic analysis of black-stained plaques in permanent dentition

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dc.contributor.authorILGIN, CAN
dc.contributor.authorsCelik, Zeynep Ceren; Cakiris, Aris; Yanikoglu, Funda; Abaci, Neslihan; Ekmekci, Sema Sirma; Ilgin, Can; Celik, Halil; Tagtekin, Dilek
dc.date.accessioned2022-03-12T22:58:24Z
dc.date.available2022-03-12T22:58:24Z
dc.date.issued2021
dc.description.abstractObjectives: We aimed to determine the aetiologic agent responsible for black staining of permanent dentition using next-generation sequencing and determine the relationship between caries and black stains. Materials and methods: A total of 52 systemically healthy patients with black-stained and caries-free (n = 13), black-stained and carious (n = 13), black stain-free and caries-free (n = 13), and black stain-free and carious (n = 13) teeth were enrolled in the study. The International Caries Detection and Assessment System (ICDAS II) was used for caries classification. Between 08:00 and 10:00, supragingival plaque samples were collected after a minimum of 8-12 h of accumulation and DNA samples were isolated. The samples were processed using the ZymoBIOMICS (TM) Service. Bioinformatics analysis was performed using mothur at usegalaxy.org. Data were analysed statistically using the Pearson chi-square and Fisher tests. Results: The number of caries-free teeth (ICDAS 0, 1, and 2) was significantly higher in patients with black stains (p = 0.007).Capnocytophaga (4.8 %), Corynebacterium (3.9 %), and Neisseria (5.4 %) species were the most abundant among all black-stained plaques (carious and caries-free) (p < 0.05). Capnocytophaga (10.8 %), Cardiobacterium (3.6 %), and Rothia (1.72 %) species were detected in the black-stained plaques of caries-free patients (p < 0.05). Conclusion: This study is one of the first studies examining the microbial composition of dental plaques with black staining in carious and caries-free adult patients using next generation sequencing technology. In the presence of black staining, plaques have an ultimate complex microbial structure. A lower caries burden was noted in the presence of black staining.
dc.identifier.doi10.1016/j.archoralbio.2021.105171
dc.identifier.eissn1879-1506
dc.identifier.issn0003-9969
dc.identifier.pubmed34058724
dc.identifier.urihttps://hdl.handle.net/11424/237184
dc.identifier.wosWOS:000659789300008
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofARCHIVES OF ORAL BIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDental caries
dc.subjectBlack stain
dc.subjectNext generation sequencing
dc.subjectOral microbiome
dc.subjectDENTAL-CARIES
dc.subjectCHILDREN
dc.titleMetagenomic analysis of black-stained plaques in permanent dentition
dc.typearticle
dspace.entity.typePublication
local.avesis.idcf5087fb-7b69-4278-a597-95ce75aeb586
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber105171
local.journal.numberofpages5
oaire.citation.titleARCHIVES OF ORAL BIOLOGY
oaire.citation.volume128
relation.isAuthorOfPublication380f62e4-4745-4e22-b9c9-67cb0373648d
relation.isAuthorOfPublication.latestForDiscovery380f62e4-4745-4e22-b9c9-67cb0373648d

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