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Antitumor and antimetastatic effects of walnut oil in esophageal adenocarcinoma cells

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dc.contributor.authorŞAHİN, ALİ
dc.contributor.authorsBatirel, Saime; Yilmaz, Ayse Mine; Sahin, Ali; Perakakis, Nikolaos; Ozer, Nesrin Kartal; Mantzoros, Christos S.
dc.date.accessioned2022-03-12T22:26:21Z
dc.date.available2022-03-12T22:26:21Z
dc.date.issued2018
dc.description.abstractBackground: Walnuts contain many components including specific fatty acids, which could be active against cancer. Even though the anticarcinogenic effect of some of the individual fatty acids in walnut oil has been described, the effect of walnut oil itself on esophageal cancer cells hasn't yet been investigated. Objective: We aimed to investigate whether walnut oil affects tumor growth and metastatic potential in esophageal cancer cells. Methods: The human esophageal adenocarcinoma cell line, OE19, was treated with different doses of walnut oil and cell viability, apoptosis/necrosis and cell cycle analyses were performed using WST-1 assay and flow cytometry respectively. Adhesion, colony formation and wound healing assays were performed to assess the antimetastatic effects of walnut oil. NFkB expression was evaluated with western blot analysis. Results: Walnut oil decreased the cell viability of esophageal cancer cells in a dose-dependent manner. 20 mg/mL walnut oil reduced cell viability by similar to 50% when compared with control. The analysis revealed that necrosis and accumulation of cells in G0/G1 phase was induced in the cells treated with high doses of walnut oil. It also down-regulated the protein levels of NFkB. Walnut oil suppressed the adhesion, migration and colony formation of the cells. Conclusions: High-dose short-term administration of walnut oil reduces the cell viability and metastatic ability of esophageal cancer cells, while exhibiting anticarcinogenic effect by inducing necrosis and cell cycle arrest at the G0/G1 phase, probably through suppression of the NFkB pathway. These data indicate that walnut oil, and by extension walnut consumption, may have beneficial effects in esophageal cancer in humans. This should be tested by clinical trials in the future. (C) 2017 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
dc.identifier.doi10.1016/j.clnu.2017.10.016
dc.identifier.eissn1532-1983
dc.identifier.issn0261-5614
dc.identifier.pubmed29126716
dc.identifier.urihttps://hdl.handle.net/11424/235053
dc.identifier.wosWOS:000455069400045
dc.language.isoeng
dc.publisherCHURCHILL LIVINGSTONE
dc.relation.ispartofCLINICAL NUTRITION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEsophageal cancer
dc.subjectWalnut oil
dc.subjectAntitumor
dc.subjectAntimetastatic
dc.subjectALPHA-LINOLENIC ACID
dc.subjectCANCER GROWTH
dc.subjectFATTY-ACIDS
dc.subjectPREVENTION
dc.subjectRISK
dc.subjectINHIBITION
dc.subjectMECHANISMS
dc.subjectMICE
dc.subjectDIET
dc.titleAntitumor and antimetastatic effects of walnut oil in esophageal adenocarcinoma cells
dc.typearticle
dspace.entity.typePublication
local.avesis.idc3508002-33a3-413d-816b-4e0b62d77ba9
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
local.journal.quartileQ1
oaire.citation.endPage2171
oaire.citation.issue6
oaire.citation.startPage2166
oaire.citation.titleCLINICAL NUTRITION
oaire.citation.volume37
relation.isAuthorOfPublicationdda71138-8ce4-4265-89b2-73bc94786a4f
relation.isAuthorOfPublication.latestForDiscoverydda71138-8ce4-4265-89b2-73bc94786a4f

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