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Performance Evaluation of a New Coulometric Endpoint Method in Sweat Testing and Its Comparison With Classic Gibson&Cooke and Chloridometer Methods in Cystic Fibrosis

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dc.contributor.authorKARADAĞ, BÜLENT TANER
dc.contributor.authorsGokdemir, Yasemin; Vatansever, Pinar; Karadag, Bulent; Seyrekel, Tuncay; Baykan, Ozgur; Ikizoglu, Nilay Bas; Ersu, Refika; Karakoc, Fazilet; Haklar, Goncagul
dc.date.accessioned2022-03-14T08:41:59Z
dc.date.available2022-03-14T08:41:59Z
dc.date.issued2018-05-22
dc.description.abstractBackground: The objective of the study was to assess the diagnostic efficacy of the coulometric endpoint method and compare it with classic Gibson&Cooke and chloridometer methods. Methods: This study is a prospective clinical study comparing two conventional sweat testing methods with the coulometric endpoint method in previously diagnosed cystic fibrosis (CF) patients and a non-CF control group. All individuals underwent two simultaneous sweat collections. One sample of sweat, collected by the CF Delta collector coil system, was analyzed by two methods: the titrimetric Cl- measurement (Sherwood (R) Chloridometer 926S, Sherwood Scientific Ltd., Cambridge, UK) and the coulometric endpoint method (CF Delta Collection System (R), UTSAT/Turkey); the second sample was collected from the other forearm by the Gibson&Cooke method and the collected sweat was analyzed by manual titration in accordance with the Schales&Schales method. Within-run and between-run imprecisions were evaluated via Cl- concentrations of 40, 70, and 130 mmol/L samples. Results: One hundred and seventy (60 CF and 110 controls) subjects were included in the study. All three sweat test methods discriminated CF subjects from the healthy individuals. The mean difference between the coulometric endpoint and titrimetric Cl - measurement methods was -1.5 mmol/L, (95% confidence limits of agreement, ranging from -8.9 to 15.9 mmol/L); the mean difference between manual titration vs. coulometric endpoint methods was 12.8 mmol/L, (95% confidence limits of agreement ranging from -9.7 to 45.3 mmol/L) and the mean difference between the manual titration and titrimetric Cl - measurement methods was 11.3 mmol/L, (95% confidence limits of agreement ranging from -7.8 to 40.5 mmol/L) based on a Bland-Altman analysis. In the Receiver operating characteristic (ROC) analysis, made on the basis that Cl- concentration values <40 mmol/L exclude the CF diagnosis, the coulometric endpoint method resulted in 96.7% sensitivity and 100% specificity for a cut-off value of 58.5 mmol/L (AUC: 0.994; 95% CI = 0.986-1.000; p < 0.001). Conclusions: The coulometric endpoint method can be as reliable as quantitative sweat Cl- analysis and may be considered as a definitive diagnostic tool for CF.
dc.identifier.doi10.3389/fped.2018.00133
dc.identifier.issn2296-2360
dc.identifier.pubmed29876336
dc.identifier.urihttps://hdl.handle.net/11424/242148
dc.identifier.wosWOS:000432835400001
dc.language.isoeng
dc.publisherFRONTIERS MEDIA SA
dc.relation.ispartofFRONTIERS IN PEDIATRICS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectsweat test
dc.subjectcystic fibrosis
dc.subjectcoulometric endpoint system
dc.subjectdiagnosis
dc.subjectchloride
dc.subjectDIAGNOSIS
dc.subjectCONDUCTIVITY
dc.subjectSODIUM
dc.titlePerformance Evaluation of a New Coulometric Endpoint Method in Sweat Testing and Its Comparison With Classic Gibson&Cooke and Chloridometer Methods in Cystic Fibrosis
dc.typearticle
dspace.entity.typePublication
local.avesis.idf0c322ca-ae1d-463e-9607-8c36cb979e55
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber133
local.journal.numberofpages8
local.journal.quartileQ2
oaire.citation.titleFRONTIERS IN PEDIATRICS
oaire.citation.volume6
relation.isAuthorOfPublication218b489d-97ab-49dd-9db6-0c2a732f1999
relation.isAuthorOfPublication.latestForDiscovery218b489d-97ab-49dd-9db6-0c2a732f1999

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