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In Vivo Effects on Stress Protein, Genotoxicity, and Oxidative Toxicity Parameters in Oreochromis niloticus Tissues Exposed to Thiamethoxam

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dc.contributor.authorKARĞIN, DİCLE
dc.contributor.authorsTemiz, Ozge; Kargin, Dicle; Cogun, Hikmet Y.
dc.date.accessioned2022-03-12T22:57:22Z
dc.date.available2022-03-12T22:57:22Z
dc.date.issued2021
dc.description.abstractThis study aimed to observe the effect of toxicity of the pesticide thiamethoxam (TMX) at sublethal concentrations in the liver and brain of Oreochromis niloticus. In the experiment, fish were exposed to 50, 100, and, 150 mg/L with thiamethoxam for 48 h and 15 days. The superoxide dismutase (SOD), catalase (CAT), glutathione S-transferaz (GST), glutathione peroxidase (GPx), and ethoxyresorufin-O-deethylase (EROD) activities; and thiobarbituric acid reactive substance (TBARS), heat shock proteins 70 (HSP70), glutathione (GSH), and genotoxicity parameter 8-hydroxy-2 '-deoxyguanosine (8-OHdG) were analyzed by spectrophotometric methods and ELISA techniques. Depending on time and dose in TMX exposure in liver tissue, a significant decrease in GSH level; an increase in SOD, GST, GPx, and EROD enzyme activities; and HSP70, TBARS, and 8-OHdG levels was determined. In brain tissue, SOD, GST, and EROD enzyme activities, an increase in HSP70, TBARS, and 8-OHdG levels, and a decrease in CAT enzyme activity and GSH levels were determined. In this study, TMX in the concentrations used showed that changes in oxidative stress biomarkers, genotoxicity parameter 8-OHdG levels, and HSP70 levels caused toxic effects in the model organism. As a result of the study, the changes and protective effects of the antioxidant system and stress proteins at the cellular level were determined in sublethal doses of toxic effects caused by TMX in the vital organs of the organism. In this toxicological study, TMX exposure resulted in toxicity to O. niloticus liver and brain tissues, in addition, responses of biomarkers to time and concentrations were determined. As a result of this study, the potential toxic effects of the commonly used pesticide TMX will reveal both the ecological risks of the aquatic organism and the basic data of the safety and risk assessments of O. niloticus consumed as food for human health.
dc.identifier.doi10.1007/s11270-021-05101-7
dc.identifier.eissn1573-2932
dc.identifier.issn0049-6979
dc.identifier.urihttps://hdl.handle.net/11424/237030
dc.identifier.wosWOS:000651805500001
dc.language.isoeng
dc.publisherSPRINGER INTERNATIONAL PUBLISHING AG
dc.relation.ispartofWATER AIR AND SOIL POLLUTION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPesticide
dc.subjectThiamethoxam
dc.subjectOreochromis niloticus
dc.subjectHSP70
dc.subject8-OHdG
dc.subjectFRESH-WATER FISH
dc.subjectHEAT-SHOCK PROTEINS
dc.subjectDNA-DAMAGE
dc.subjectCHANNA-PUNCTATUS
dc.subjectNILE-TILAPIA
dc.subjectACETYLCHOLINESTERASE ACTIVITY
dc.subjectANTIOXIDANT SYSTEM
dc.subjectLIPID-PEROXIDATION
dc.subjectCARASSIUS-AURATUS
dc.subjectAQUATIC ORGANISMS
dc.titleIn Vivo Effects on Stress Protein, Genotoxicity, and Oxidative Toxicity Parameters in Oreochromis niloticus Tissues Exposed to Thiamethoxam
dc.typearticle
dspace.entity.typePublication
local.avesis.idde887830-5134-40a5-87ae-186e4e6144f1
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.articlenumber221
local.journal.numberofpages17
oaire.citation.issue6
oaire.citation.titleWATER AIR AND SOIL POLLUTION
oaire.citation.volume232
relation.isAuthorOfPublicationf2244808-5dab-40ea-a764-638a8558ce3b
relation.isAuthorOfPublication.latestForDiscoveryf2244808-5dab-40ea-a764-638a8558ce3b

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