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The effect of topical insulin application on the healing of acute tympanic membrane perforations: a histopathologic study

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dc.contributor.authorBOZKURT, SÜHEYLA
dc.contributor.authorsEken, Mehmet; Ates, Gunay; Sanla, Araf; Evren, Cenk; Bozkurt, Suheyla
dc.date.accessioned2022-03-12T17:32:39Z
dc.date.available2022-03-12T17:32:39Z
dc.date.issued2007
dc.description.abstractThe aim of the present study was to investigate the effects of insulin on the healing of acute traumatic tympanic membrane (TM) perforations by observing the duration of perforation closure clinically and by examining the TM thickness, fibroblastic reaction, neovascularization and collagenization histologically. In all, 24 adult healthy guinea pigs were used. TM perforations, about 2 mm in diameter were made in the pars tensa of each ear. The perforations in the right ears of the guinea pigs (study group) were treated with 1 IU of regular insulin (Humulin-R-(R) 100 IU/ml, Lilly) daily topically. The left ears of the guinea pigs (control group) were treated with saline solution daily. The treatments were continued until the each perforation closed. The animals were examined with otomicroscopy at first, third, fifth and seventh days. TM specimens were obtained after the animals were decapitated under deep anesthesia by an intraperitoneal injection of sodium pentobarbital (0.5 ml/100 g) 3 and 7 days after wounding. Histologically, the epithelial and mucosal layers were examined. Finger-like projections, the edema of the LP, neovascularization of LP, fibroblastic activity of LP, inflammatory cell presence of LP, collagenization ratios of LP were evaluated. In the study group; LP fibroblastic reaction positivity and LP collagenization positivity was significantly higher than the control group (P < 0.001 and P < 0.05). Topical insulin treatment may be more beneficial in the treatment of atrophic membrane, which is a sequel of perforation, when tried in various dosages and time intervals.
dc.identifier.doi10.1007/s00405-007-0303-3
dc.identifier.issn0937-4477
dc.identifier.pubmed17431656
dc.identifier.urihttps://hdl.handle.net/11424/228648
dc.identifier.wosWOS:000248326900004
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofEUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecttopical insulin
dc.subjectmyringotomy
dc.subjectwound healing
dc.subjectperforation
dc.titleThe effect of topical insulin application on the healing of acute tympanic membrane perforations: a histopathologic study
dc.typearticle
dspace.entity.typePublication
local.avesis.idca2cadf9-c18d-4578-ad49-b89cd0c20d61
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages4
oaire.citation.endPage1002
oaire.citation.issue9
oaire.citation.startPage999
oaire.citation.titleEUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
oaire.citation.volume264
relation.isAuthorOfPublicationdd039cf9-dfb8-4af0-b879-0b88c3a852fa
relation.isAuthorOfPublication.latestForDiscoverydd039cf9-dfb8-4af0-b879-0b88c3a852fa

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