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Phase-amplitude coupled persistent theta and gamma oscillations in rat primary motor cortex in vitro

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dc.contributor.authorÖZKAN, MAZHAR
dc.contributor.authorsJohnson, Nicholas W.; Ozkan, Mazhar; Burgess, Adrian P.; Prokic, Emma J.; Wafford, Keith A.; O'Neill, Michael J.; Greenhill, Stuart D.; Stanford, Ian M.; Woodhall, Gavin L.
dc.date.accessioned2022-03-14T08:22:54Z
dc.date.available2022-03-14T08:22:54Z
dc.date.issued2017-06
dc.description.abstractIn vivo, theta (4-7 Hz) and gamma (30-80 Hz) neuronal network oscillations are known to coexist and display phase-amplitude coupling (PAC). However, in vitro, these oscillations have for many years been studied in isolation. Using an improved brain slice preparation technique we have, using co-application of carbachol (10 mu M) and kainic acid (150 nM), elicited simultaneous theta (6.6 +/- 0.1 Hz) and gamma (36.6 +/- 0.4 Hz) oscillations in rodent primary motor cortex (M1). Each oscillation showed greatest power in layer V. Using a variety of time series analyses we detected significant cross-frequency coupling in 74% of slice preparations. Differences were observed in the pharmacological profile of each oscillation. Thus, gamma oscillations were reduced by the GABAA receptor antagonists, gabazine (250 nM and 2 mu M), and picrotoxin (50 mu M) and augmented by AMPA receptor antagonism with SYM2206 (20 mu M). In contrast, theta oscillatory power was increased by gabazine, picrotoxin and SYM2206. GABA(B) receptor blockade with CGP55845 (5 mu M) increased both theta and gamma power, and similar effects were seen with diazepam, zolpidem, MK801 and a series of metabotropic glutamate receptor antagonists. Oscillatory activity at both frequencies was reduced by the gap junction blocker carbenoxolone (200 mu M) and by atropine (5 mu). These data show theta and gamma oscillations in layer V of rat M1 in vitro are cross-frequency coupled, and are mechanistically distinct. The development of an in vitro model of phase-amplitude coupled oscillations will facilitate further mechanistic investigation of the generation and modulation of coupled activity in mammalian cortex. (C) 2017 The Authors. Published by Elsevier Ltd.
dc.identifier.doi10.1016/j.neuropharm.2017.04.009
dc.identifier.eissn1873-7064
dc.identifier.issn0028-3908
dc.identifier.pubmed28400257
dc.identifier.urihttps://hdl.handle.net/11424/241665
dc.identifier.wosWOS:000403513800013
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofNEUROPHARMACOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNeuronal network oscillations
dc.subjectM1
dc.subjectTheta
dc.subjectGamma
dc.subjectPhase amplitude coupling
dc.subjectPARVALBUMIN-POSITIVE INTERNEURONS
dc.subjectNITRIC-OXIDE SYNTHASE
dc.subjectENTORHINAL CORTEX
dc.subjectHIPPOCAMPAL-FORMATION
dc.subjectNETWORK OSCILLATIONS
dc.subjectRECEPTOR ACTIVATION
dc.subjectBEHAVING RAT
dc.subjectNEURONAL OSCILLATIONS
dc.subjectCORTICAL OSCILLATIONS
dc.subjectSOMATOSENSORY CORTEX
dc.titlePhase-amplitude coupled persistent theta and gamma oscillations in rat primary motor cortex in vitro
dc.typearticle
dspace.entity.typePublication
local.avesis.id04dd0d48-c481-4813-ae94-60e41260f1fe
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages16
local.journal.quartileQ1
oaire.citation.endPage156
oaire.citation.startPage141
oaire.citation.titleNEUROPHARMACOLOGY
oaire.citation.volume119
relation.isAuthorOfPublicatione76ec200-0365-4842-a165-2bec4142aeda
relation.isAuthorOfPublication.latestForDiscoverye76ec200-0365-4842-a165-2bec4142aeda

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