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The Stimulatory Effect of Mannitol on Levan Biosynthesis: Lessons from Metabolic Systems Analysis of Halomonas smyrnensis AAD6(T)

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsAtes, Ozlem; Arga, Kazim Y.; Oner, Ebru Toksoy
dc.date.accessioned2022-03-12T18:08:25Z
dc.date.available2022-03-12T18:08:25Z
dc.date.issued2013
dc.description.abstractHalomonas smyrnensis AAD(T) is a halophilic, gram-negative bacterium that can efficiently produce levan from sucrose as carbon source via levansucrase activity. However, systems-based approaches are required to further enhance its metabolic performance for industrial application. As an important step toward this goal, the genome-scale metabolic network of Chromohalobacter salexigens DSM3043, which is considered a model organism for halophilic bacteria, has been reconstructed based on its genome annotation, physiological information, and biochemical information. In the present work, the genome-scale metabolic network of C. salexigens was recruited, and refined via integration of the available biochemical, physiological, and phenotypic features of H. smyrnensis AAD6(T). The generic metabolic model, which comprises 1,393 metabolites and 1,108 reactions, was then systematically analyzed in silico using constraints-based simulations. To elucidate the relationship between levan biosynthesis and other metabolic processes, an enzyme-graph representation of the metabolic network and a graph decomposition technique were employed. Using the concept of control effective fluxes, significant links between several metabolic processes and levan biosynthesis were estimated. The major finding was the elucidation of the stimulatory effect of mannitol on levan biosynthesis, which was further verified experimentally via supplementation of mannitol to the fermentation medium. The optimal concentration of 30 g/L mannitol supplemented to the 50 g/L sucrose-based medium resulted in a twofold increase in levan production in parallel with increased sucrose hydrolysis rate, accumulated extracellular glucose, and decreased fructose uptake rate. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1386-1397, 2013
dc.identifier.doi10.1002/btpr.1823
dc.identifier.eissn1520-6033
dc.identifier.issn8756-7938
dc.identifier.pubmed24123998
dc.identifier.urihttps://hdl.handle.net/11424/231149
dc.identifier.wosWOS:000328217100005
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofBIOTECHNOLOGY PROGRESS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectlevan
dc.subjectmannitol
dc.subjectHalomonas smyrnensis
dc.subjectmetabolic network
dc.subjectgenome-scale modeling
dc.subjectFLUX BALANCE ANALYSIS
dc.subjectEXOPOLYSACCHARIDES
dc.subjectLEVANSUCRASE
dc.subjectNETWORK
dc.subjectGENOME
dc.subjectSTRAIN
dc.subjectRECONSTRUCTION
dc.subjectIDENTIFICATION
dc.subjectEXTREMOPHILES
dc.subjectFERMENTATION
dc.titleThe Stimulatory Effect of Mannitol on Levan Biosynthesis: Lessons from Metabolic Systems Analysis of Halomonas smyrnensis AAD6(T)
dc.typearticle
dspace.entity.typePublication
local.avesis.id72d4937e-1b50-4068-86ec-5962a60b28d1
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages12
oaire.citation.endPage1397
oaire.citation.issue6
oaire.citation.startPage1386
oaire.citation.titleBIOTECHNOLOGY PROGRESS
oaire.citation.volume29
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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