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Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters

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dc.contributor.authorYÜKSEL, MERAL
dc.contributor.authorsAlkan, Ilter; Yuksel, Meral; Canat, Halil Lutfi; Atalay, Hasan Anil; Can, Osman; Ozveri, Hakan; Basar, Mehmet Murad
dc.date.accessioned2022-03-14T09:04:18Z
dc.date.available2022-03-14T09:04:18Z
dc.date.issued2018
dc.description.abstractPurpose: To investigate the pathophysiological role of superoxide anion and total reactive oxygen species (ROS) production by the spermatozoa of men with varicocele and its relationship with varicocele grade and semen parameters. Materials and Methods: This prospective study included 34 men with grade II-III varicocele, regardless of their fertility status. The control group consisted of 13 healthy men. Semen characteristics were examined according to the 2010 World Health Organization criteria. The swim-up method was used for sperm preparation. Total ROS and superoxide anion production was assayed by luminol-and lucigenin-dependent chemiluminescence (CL), respectively. Results: The men with varicocele had significantly higher total ROS and superoxide anion levels than the healthy control subjects (2.9 +/- 0.4 relative light unit (RLU) vs. 2.4 +/- 0.1 RLU, p=0.001 for luminol-dependent CL and 2.8 +/- 0.4 RLU vs. 2.3 +/- 0.2 RLU, p=0.002 for lucigenin-dependent CL). Cases of grade III varicocele had significantly higher superoxide anion and total ROS levels than grade II cases and control subjects (p<0.001). Superoxide anion and total ROS levels were negatively correlated with all semen parameters. Conclusions: The superoxide anion levels produced by spermatozoa were significantly higher in varicocele patients than in control subjects. ROS production was related to increased varicocele grade, impaired semen concentration, and abnormal morphology in men with varicocele. Our findings suggest that superoxide anion overproduction may be an important step in the cascade of ROS-related damage to spermatozoa, resulting in impaired semen parameters in patients with varicocele.
dc.identifier.doi10.5534/wjmh.180028
dc.identifier.eissn2287-4690
dc.identifier.issn2287-4208
dc.identifier.pubmed30168299
dc.identifier.urihttps://hdl.handle.net/11424/242385
dc.identifier.wosWOS:000443344400010
dc.language.isoeng
dc.publisherKOREAN SOC SEXUAL MEDICINE & ANDROLOGY
dc.relation.ispartofWORLD JOURNAL OF MENS HEALTH
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOxidative stress
dc.subjectReactive oxygen species
dc.subjectSpermatozoa
dc.subjectSuperoxides
dc.subjectVaricocele
dc.subjectOXIDATIVE STRESS
dc.subjectINFERTILITY
dc.subjectPATHOPHYSIOLOGY
dc.subjectFRAGMENTATION
dc.subjectPATHOGENESIS
dc.subjectGENERATION
dc.subjectDIAGNOSIS
dc.subjectCRITERIA
dc.subjectHYPOXIA
dc.titleSuperoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters
dc.typearticle
dspace.entity.typePublication
local.avesis.id9240fdb4-b303-4930-bbff-e7155cb8f6ef
local.import.packageSS16
local.indexed.atWOS
local.indexed.atPUBMED
local.journal.numberofpages8
local.journal.quartileQ2
oaire.citation.endPage262
oaire.citation.issue3
oaire.citation.startPage255
oaire.citation.titleWORLD JOURNAL OF MENS HEALTH
oaire.citation.volume36
relation.isAuthorOfPublication8b13d479-2f3b-4180-bc71-7ad5a5625f1b
relation.isAuthorOfPublication.latestForDiscovery8b13d479-2f3b-4180-bc71-7ad5a5625f1b

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