Publication:
The anti-inflammatory effect of leptin on experimental colitis: involvement of endogenous glucocorticoids

Simple item page
dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsCakir, B; Bozkurt, A; Ercan, F; Yegen, BC
dc.date.accessioned2022-03-12T17:16:17Z
dc.date.available2022-03-12T17:16:17Z
dc.date.issued2004
dc.description.abstractThe present study was designed to compare the effect of leptin on acute colonic inflammation with that of acute stress exposure, which acts via the hypothalamic-pituitary-adrenal (HPA) axis. Sprague-Dawley rats of both sexes were administered intrarectally with acetic acid. Either leptin (10 mug/kg; i.p.) or saline was injected immediately before and 6 h after the induction of colitis. A group of rats was exposed to water avoidance stress (WAS) for 30 min at the 6th h of colitis induction. RU-486 (2 mg/kg; i.p.), a glucocorticoid receptor antagonist, was injected intraperitoneally, at 12 and 1 h before the initial leptin injection, and at 1 h before the second leptin injection or exposure to WAS. Rats were decapitated at 24 h and the distal 8 cm of,the colon were removed for macroscopic and microscopic scoring, determination of tissue wet weight index (WI) and tissue myeloperoxidase activity (MPO). Acetic acid-induced colitis significantly increased macroscopic and microscopic damage scores, WI and MPO, compared to control group. Exposure to acute WAS or treatment with leptin reduced the elevations in damage scores, WI and MPO induced by colitis, but no additive inhibitory effect was observed when WAS and leptin were applied together. RU-486 treatment reversed the inhibitory effects of leptin or WAS on colonic inflammation. Our results demonstrate that exogenous leptin mimics the effects of HPA axis activation on colitis-induced inflammatory process. The results also suggest that the anti-inflammatory effect of leptin involves a tissue neutrophil-dependent mechanism and is dependent on the release of glucocorticoids. (C) 2003 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.peptides.2003.11.005
dc.identifier.eissn1873-5169
dc.identifier.issn0196-9781
dc.identifier.pubmed15003361
dc.identifier.urihttps://hdl.handle.net/11424/227505
dc.identifier.wosWOS:000220267900013
dc.language.isoeng
dc.publisherELSEVIER SCIENCE INC
dc.relation.ispartofPEPTIDES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecthypothalamo-pituitary-adrenal axis
dc.subjectwater avoidance stress
dc.subjectACTH
dc.subjectacetic acid colitis
dc.subjectmyeloperoxidase activity
dc.subjectGASTROINTESTINAL-TRACT
dc.subjectEXPRESSION
dc.subjectSTRESS
dc.subjectRATS
dc.subjectCHOLECYSTOKININ
dc.subjectINFLAMMATION
dc.subjectMECHANISMS
dc.subjectGENE
dc.subjectGASTROPROTECTION
dc.subjectSUSCEPTIBILITY
dc.titleThe anti-inflammatory effect of leptin on experimental colitis: involvement of endogenous glucocorticoids
dc.typearticle
dspace.entity.typePublication
local.avesis.id95c296e0-0da0-4108-a723-def51c64867c
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
oaire.citation.endPage104
oaire.citation.issue1
oaire.citation.startPage95
oaire.citation.titlePEPTIDES
oaire.citation.volume25
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

Files

Collections

Research Outputs