Publication: Design of cinnamaldehyde- and gentamicin-loaded double-layer corneal nanofiber patches with antibiofilm and antimicrobial effects
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Date
2023-07-26
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Abstract
: In this study, two-layer poly(vinyl alcohol)/gelatin (PVA/GEL) nanofiber
patches containing cinnamaldehyde (CA) in the first layer and gentamicin (GEN) in the
second layer were produced by the electrospinning method. The morphology, chemical
structures, and thermal temperatures of the produced pure (PVA/GEL), CA-loaded (PVA/
GEL/CA), GEN-loaded (PVA/GEL/GEN), and combined drug-loaded (PVA/GEL/CA/
GEN) nanofiber patches were determined by scanning electron microscopy (SEM), Fourier
transform infrared spectroscopy, and differential scanning calorimetry, respectively. Their
mechanical properties, swelling and degradation behavior, and drug release kinetics were
investigated. SEM images showed that both drug-free and drug-loaded nanofiber patches
possess smooth and monodisperse structures, and nanofiber size increase occurred as the
amount of drug increased. The tensile test results showed that the mechanical strength
decreased as the drug was loaded. According to the drug release results, CA release ended at
the 96th hour, while GEN release continued until the 264th hour. The antibacterial and
antibiofilm activities of PVA/GEL, PVA/GEL/CA, PVA/GEL/GEN, and PVA/GEL/CA/GEN nanofiber patches against
Pseudomonas aeruginosa and Staphylococcus aureus were evaluated. Results showed that PVA/GEL/GEN and PVA/GEL/CA/GEN
nanofiber patches have excellent antibacterial and antibiofilm activities. Moreover, all materials were biocompatible, with no
cytotoxic effects in the mammalian cell model for 8 days. PVA/GEL/GEN nanofiber patches were the most promising material for a
high cell survival ratio, which was confirmed by SEM images. This research aims to develop an alternative method to stop and treat
the rapid progression of bacterial keratitis.
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Keywords
Absorption, Antimicrobial agents, Biofilms, Drug release, Nanofibers
Citation
Cesur S., Ilhan E., Tut T. A., Kaya E., Dalbayrak B., Bosgelmez-Tinaz G., Arısan E. D., Gunduz O., Kijeńska-Gawrońska E., "Design of Cinnamaldehyde- and Gentamicin-Loaded Double-Layer Corneal Nanofiber Patches with Antibiofilm and Antimicrobial Effects.", ACS omega, cilt.8, sa.31, ss.28109-28121, 2023