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Flow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia

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dc.contributor.authorÖZSAVCI, DERYA
dc.contributor.authorsOzsavci, D; Yardimci, T; Demirel, GY; Demiralp, E; Uras, F; Onder, E
dc.date.accessioned2022-03-12T17:16:15Z
dc.date.available2022-03-12T17:16:15Z
dc.date.issued2002
dc.description.abstractIn this study, platelet glycoprotein (Gp) receptor numbers were measured by a flow cytometric assay using Cytoquant Gp in seven hypercholesterolemic and five normal subjects. Thrombin receptor agonist peptide (TRAP) was used to activate platelets. In hypercholesterolemia the Gp receptor numbers per resting platelet were found to be: 38 629 +/- 8538 (GpIIb/IIIa), 22 269 +/- 5628 (GpIb), 37 037 +/- 9810 (GpIIIa), 224 +/- 504 (CD62-P). After activation, receptor numbers were determined to be: 56 399 +/- 9003 (GpIIb/ IIIa), 10 970 +/- 5319 (GpIb), 50 715 +/- 7904 (GpIIIa), 1222 +/- 687 (P-selectin). In the normal group before the activation, receptor numbers were: 43 828 +/- 8862 (GpIIb/ IIIa), 22 166 +/- 3847 (GpIb), 42 351 +/- 1049 (GpIIIa), 62 +/- 139 (CD62-P), After activation, receptor numbers were determined to be: 60 573 +/- 4294 (GpIIb/ IIIa), 13 003 +/- 4118 (GpIb), 52 067 +/- 1039 (GpIIIa), 3608 +/- 1508 (CD62-P). In hypercholesterolemic subjects, GpIIb/ IIIa and GpIIIa receptor numbers on activated platelets increased significantly, whereas P-selectin numbers remained unchanged. However, the GpIb levels decreased significantly. In the control group, after activation, GpIIb/ IIIa and P-selectin receptors increased significantly, GpIIIa receptor numbers did not change significantly, whereas GpIb receptor numbers decreased significantly. When the GpIIb/ IIIa, GpIb, GpIIIa receptor numbers of the control group and hypercholesterolemic group were compared before and after activation, no significant changes were observed (P>0.05). But P-selectin receptor numbers were significantly decreased in hypercholesterolemic patients compared to normals following TRAP activation (P<0.05). In this study, the effect of hypercholesterolemia on platelet function was observed. The striking observation about present study was the marked decrease in P-selectin expression after activation in the hypercholesterolemics compared to normals. This finding suggests some sort of platelet dysfunction in these individuals.
dc.identifier.doi10.1080/09537100220146866
dc.identifier.eissn1369-1635
dc.identifier.issn0953-7104
dc.identifier.pubmed12189024
dc.identifier.urihttps://hdl.handle.net/11424/227483
dc.identifier.wosWOS:000177196500005
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS INC
dc.relation.ispartofPLATELETS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectACTIVATED PLATELETS
dc.subjectWHOLE-BLOOD
dc.subjectIIB-IIIA
dc.subjectSURFACE GLYCOPROTEINS
dc.subjectMONOCLONAL-ANTIBODY
dc.subjectIN-VITRO
dc.subjectFIBRINOGEN
dc.subjectTHROMBIN
dc.subjectMECHANISMS
dc.subjectANTIGENS
dc.titleFlow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia
dc.typearticle
dspace.entity.typePublication
local.avesis.id74048203-e968-4a13-987d-f4c56df14eae
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage229
oaire.citation.issue4
oaire.citation.startPage223
oaire.citation.titlePLATELETS
oaire.citation.volume13
relation.isAuthorOfPublication68ec1222-da29-4568-a8e5-56ebd6b48020
relation.isAuthorOfPublication.latestForDiscovery68ec1222-da29-4568-a8e5-56ebd6b48020

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