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Transcriptomic analysis displays the effect of (-)-roemerine on the motility and nutrient uptake in Escherichia coli

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsAyyildiz, Dilara; Arga, Kazim Yalcin; Avci, Fatma Gizem; Altinisik, Fatma Ece; Gurer, Caglayan; Toplan, Gizem Gulsoy; Kazan, Dilek; Wozny, Katharina; Bruegger, Britta; Mertoglu, Bulent; Akbulut, Berna Sariyar
dc.date.accessioned2022-03-12T22:23:47Z
dc.date.available2022-03-12T22:23:47Z
dc.date.issued2017
dc.description.abstractAmong the different families of plant alkaloids, (-)-roemerine, an aporphine type, was recently shown to possess significant antibacterial activity in Escherichia coli. Based on the increasing demand for antibacterials with novel mechanisms of action, the present work investigates the potential of the plant-derived alkaloid (-)-roemerine as an antibacterial in E. coli cells using microarray technology. Analysis of the genome-wide transcriptional reprogramming in cells after 60 min treatment with 100 mu g/mL (-)-roemerine showed significant changes in the expression of 241 genes (p value < 0.05 and fold change > 2). Expression of selected genes was confirmed by qPCR. Differentially expressed genes were classified into functional categories to map biological processes and molecular pathways involved. Cellular activities with roles in carbohydrate transport and metabolism, energy production and conversion, lipid transport and metabolism, amino acid transport and metabolism, two-component signaling systems, and cell motility (in particular, the flagellar organization and motility) were among metabolic processes altered in the presence of (-)-roemerine. The down-regulation of the outer membrane proteins probably led to a decrease in carbohydrate uptake rate, which in turn results in nutrient limitation. Consequently, energy metabolism is slowed down. Interestingly, the majority of the expressional alterations were found in the flagellar system. This suggested reduction in motility and loss in the ability to form biofilms, thus affecting protection of E. coli against host cell defense mechanisms. In summary, our findings suggest that the antimicrobial action of (-)-roemerine in E. coli is linked to disturbances in motility and nutrient uptake.
dc.identifier.doi10.1007/s00294-016-0673-4
dc.identifier.eissn1432-0983
dc.identifier.issn0172-8083
dc.identifier.pubmed28013396
dc.identifier.urihttps://hdl.handle.net/11424/234559
dc.identifier.wosWOS:000405310000011
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofCURRENT GENETICS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRoemerine
dc.subjectAntibacterial
dc.subjectTranscriptome
dc.subjectDrug
dc.subjectAntimicrobial resistance
dc.subjectEscherichia coli
dc.subjectOUTER-MEMBRANE PROTEINS
dc.subjectPROTEOMIC ANALYSIS
dc.subjectGENE-EXPRESSION
dc.subjectMOLECULAR-BASIS
dc.subjectMARRAB OPERON
dc.subjectRESISTANCE
dc.subjectPERMEABILITY
dc.subjectIDENTIFICATION
dc.subjectALKALOIDS
dc.subjectCURLI
dc.titleTranscriptomic analysis displays the effect of (-)-roemerine on the motility and nutrient uptake in Escherichia coli
dc.typearticle
dspace.entity.typePublication
local.avesis.ida261da54-b8f6-4d15-8a01-9eb7fc4b0e05
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages14
local.journal.quartileQ2
oaire.citation.endPage722
oaire.citation.issue4
oaire.citation.startPage709
oaire.citation.titleCURRENT GENETICS
oaire.citation.volume63
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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