Publication:
Effect of Sample Storage on Stability of Salivary Glutathione, Lipid Peroxidation Levels, and Tissue Factor Activity

Simple item page
dc.contributor.authorYARAT, AYŞEN
dc.contributor.authorsEmekli-Alturfan, Ebru; Kasikci, Emel; Alturfan, A. Ata; Pisiriciler, Rabia; Yarat, Aysen
dc.date.accessioned2022-03-14T09:51:22Z
dc.date.available2022-03-14T09:51:22Z
dc.date.issued2009
dc.description.abstractSaliva samples are often required to be stored for longer periods of time either because of the project protocol or because of lack of funding for analysis. The effects of 6 months storage (fresh, 30, 60, 90 120, 150, and 180d) on the stability of salivary reduced glutathione (GSH), lipid peroxidation (LPO) and 90days of storage (fresh, 15, 30, 60, and 90d) on the stability of salivary tissue factor (TF) activity and the stability of saliva imprint samples at 20 C were evaluated in this study. Salivary GSH, malondialdehyde (MDA) levels as an index of LPO, and TF activities were determined using the methods of Beutler, Yagi, and Quick, respectively. Saliva imprint samples were stained with Giemsa and microscopically examined. Salivary GSH levels and TF MDA levels increased significantly after 6 months of storage at -20 C. Leucocyte, epithelium and bacterium cell counts did not significantly change at the end of 90 d of storage. Saliva samples may be stored up to 1 month at -20 C for LPO assay. For cytological examinations, saliva samples may be stored for 90 d at -20 C. Further studies are needed to determine the stability of salivary GSH, and salivary TF activity stored less than 30 days at -20 C. On the other hand, if saliva samples are required to be stored, to avoid the changes because of different storage periods, we recommend that they must be stored under the same circumstances and in the same time period. J. Clin. Lab. Anal. 23:93-98, 2009. (C) 2009 Wiley-Liss, Inc.
dc.identifier.doi10.1002/jcla.20296
dc.identifier.eissn1098-2825
dc.identifier.issn0887-8013
dc.identifier.pubmed19288453
dc.identifier.urihttps://hdl.handle.net/11424/243326
dc.identifier.wosWOS:000264665000005
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF CLINICAL LABORATORY ANALYSIS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectsaliva
dc.subjectstorage
dc.subjecttissue factor
dc.subjectglutathione
dc.subjectlipid peroxidation
dc.subjectHUMAN-BLOOD
dc.subjectTOXICITY
dc.subjectPLASMA
dc.subjectCELLS
dc.subjectSERUM
dc.subjectPARAMETERS
dc.subjectRESISTANCE
dc.subjectDISEASE
dc.titleEffect of Sample Storage on Stability of Salivary Glutathione, Lipid Peroxidation Levels, and Tissue Factor Activity
dc.typearticle
dspace.entity.typePublication
local.avesis.id0d9b13b7-0d85-49c8-a24b-d0c3fb30c208
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage98
oaire.citation.issue2
oaire.citation.startPage93
oaire.citation.titleJOURNAL OF CLINICAL LABORATORY ANALYSIS
oaire.citation.volume23
relation.isAuthorOfPublication46115086-6b46-4ef8-aaf1-ab8eda3e917e
relation.isAuthorOfPublication.latestForDiscovery46115086-6b46-4ef8-aaf1-ab8eda3e917e

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Emekli-Alturfan et al. - 2009 - Effect of sample storage on stability of salivary .pdf
Size:
112.28 KB
Format:
Adobe Portable Document Format
Download

Collections

Research Outputs