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Demonstration of Doublecortin Protein, a Neurogenesis Marker, at the Electron Microscopic Level

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dc.contributor.authorKAYA, ÖZLEM TUĞÇE
dc.contributor.authorsKaya, Ozlem Tugce Cilingir; Moore, Cynthia; Meshul, Charles; Sirvanci, Serap
dc.date.accessioned2022-03-12T22:23:54Z
dc.date.available2022-03-12T22:23:54Z
dc.date.issued2017
dc.description.abstractObjective: Demonstration of newly born neurons in adult brains is an important issue in terms of elucidating neurogenesis. In our study, we aimed to develop an appropriate pre-embedding microwave labeling method for the newly born neuronal marker doublecortin (DCX) protein in the hippocampal dentate gyrus (DG) region. Methods: Brains were obtained from 10-week-old C57BJ/6J male mice by perfusion fixation. Vibratome sections from brain tissues were labeled with an anti-DCX antibody using a pre-embedding microwave method. Sections stained with 3.3'-diaminobenzidine (DAB) were prepared for electron microscopic (EM) analyses using a microwave. After embedding in Epon, thin sections were obtained, observed under an electron microscope, and photographed for morphological assessments. Results: DCX labeling performed using the pre-embedding microwave method was specifically demonstrated at both light and electron microscopic levels. DCX-positive cells were localized at the subgranular zone and granular layer. Electron microscopic observations showed that DCX immunoreactivity was positive at the axons, dendrites, and somata. Conclusion: We demonstrated that the existence of DCX can be determined using the pre-embedding microwave labeling as an immunoelectron method in the DG region. Our study provides a basis for further studies on neurogenesis aiming to show DCX-immunoreactive cells using the pre-embedding DAB labeling method at the electron microscopic level.
dc.identifier.doi10.5152/clinexphealthsci.2017.115
dc.identifier.issn2459-1459
dc.identifier.urihttps://hdl.handle.net/11424/234604
dc.identifier.wosWOS:000414420500002
dc.language.isotur
dc.publisherAVES PRESS LTD
dc.relation.ispartofCLINICAL AND EXPERIMENTAL HEALTH SCIENCES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNeurogenesis
dc.subjectdoublecortin
dc.subjectpre-embedding microwave technique
dc.subjectimmunoelectron microscopy
dc.subjectCENTRAL-NERVOUS-SYSTEM
dc.subjectADULT NEUROGENESIS
dc.subjectHIPPOCAMPAL NEUROGENESIS
dc.subjectDENTATE GYRUS
dc.subjectNEURONS
dc.subjectBRAIN
dc.subjectMICROTUBULES
dc.subjectEXPRESSION
dc.subjectCELLS
dc.titleDemonstration of Doublecortin Protein, a Neurogenesis Marker, at the Electron Microscopic Level
dc.typearticle
dspace.entity.typePublication
local.avesis.ide6ba8fca-60f0-4977-950e-0316fc850103
local.import.packageSS17
local.indexed.atWOS
local.indexed.atTRDIZIN
local.journal.numberofpages5
oaire.citation.endPage10
oaire.citation.issue1
oaire.citation.startPage6
oaire.citation.titleCLINICAL AND EXPERIMENTAL HEALTH SCIENCES
oaire.citation.volume7
relation.isAuthorOfPublicationd9b3e5d1-ec49-49ec-887a-14cd84eb73f6
relation.isAuthorOfPublication.latestForDiscoveryd9b3e5d1-ec49-49ec-887a-14cd84eb73f6

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