Publication: Peripheral administration of neuropeptide W inhibits gastric emptying in rats: the role of small diameter afferent fibers and cholecystokinin receptors
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Date
2023-02-10
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Abstract
Neuropeptide W (NPW), a novel hypothalamic peptide, contributes to the central regulation of food intake and
energy balance, and suppresses feeding behavior when administered centrally. The aim of our study was to
investigate the role of peripherally administered NPW in the modulation of gastric emptying, and to evaluate the
participation of afferent fibers, cholecystokinin (CCK) receptors and gastric smooth muscle contractility in the
regulatory effects of NPW on gastric motility. In Sprague-Dawley male rats equipped with gastric fistula, gastric
emptying rate of the saline and peptone solutions was measured following subcutaneous administration of NPW
(0.1 or 5 μg/kg) preceded by subcutaneous injections of saline, CCK-1 or CCK-2 receptor antagonists. Another
group of rats with cannulas were injected subcutaneously with capsaicin for afferent denervation before
commencing emptying trials. The effect of NPW on carbachol-induced gastric contractility and the role of CCK
receptors in gastric smooth muscle contractility were also assessed in gastric strips. Peripheral injection of NPW
delayed gastric emptying rate of both caloric and non-caloric liquid test meals, while administration of CCK-1 or
CCK-2 receptor antagonists or denervation of small diameter afferents reversed NPW–induced delay in gastric
emptying. Moreover, NPW inhibited antrum contractility in the organ bath. Our results revealed that peripherally administered NPW delayed liquid emptying from the stomach via the involvement of small diameter
afferent neurons and CCK receptors, and thereby this regulatory role may contribute to its central regulatory role
in controlling food intake and energy balance.
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Keywords
Neuropeptide W, Gastric motility, Small diameter afferent nerves, Cholecystokinin receptors
Citation
Tamer S. A., Yeğen B., "Peripheral administration of neuropeptide W inhibits gastric emptying in rats: the role of small diameter afferent fibers and cholecystokinin receptors.", Neuroscience letters, ss.137122, 2023