Publication: The effects of solutions of maca (Lepidium meyenii) powder as a food/feed supplement on the viability of murine macrophage cells by digital image analysis
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Date
2021-01-01
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Abstract
Maca (Lepidium meyenii) is a tuber root plant and belong to Brassicaceae family and
recently used as a supplement in human and animal nutrition. In this study, it was aimed
to investigate the cytotoxicity of two solutions (aqueous and ethanol) of maca root
powder by digital image analysis. Maca powder was mixed in ultra distilled water and
ethanol (1:2 w/v) for 24 h at 4 °C. The mixtures were centrifuged and the supernatants
were ten-fold diluted for cytotoxic analysis of Raw 264.7 murine macrophage cells. After
seeded the cell cultures in microplates, ten-fold dilutions (from 10-1
to 10-7
) of both maca
solutions were added as six replicates for 24 h. While the aqueous maca solution
increased the number of dead cells at 10-1
(50 mg mL-1
), the ethanolic solution
statistically increased the number of dead cells at 10-1
, 10-2
and 10-3 (50, 5 and 0.5 mg
mL-1
) (P<0.01). In conclusion, the alcoholic preparation of maca powder caused a higher
cytotoxic effect on the murine macrophage cells than the aqueous preparation due to the
solvents and the dilution factor in this study.
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Keywords
Lepidium meyenii, Food/Feed Supplement, Cytotoxicity
Citation
KORKMAZ S., Parmaksız A., SAİT A., OMURTAG KORKMAZ B. İ., "The effects of solutions of maca (Lepidium meyenii) powder as a food/feed supplement on the viability of murine macrophage cells by digital image analysis", Journal of Advances in VetBio Science and Techniques, cilt.6, sa.2, ss.116-120, 2021