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Proteomics Evidence for the Activity of the Putative Antibacterial Plant Alkaloid (-)-Roemerine: Mainstreaming Omics-Guided Drug Discovery

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsGokgoz, Nilay Budeyri; Akbulut, Berna Sariyar
dc.date.accessioned2022-03-13T12:48:11Z
dc.date.available2022-03-13T12:48:11Z
dc.date.issued2015
dc.description.abstractDiscovery of new antibacterials with novel mechanisms is important to counteract the ingenious resistance mechanisms of bacteria. In this connection, omics-guided drug discovery offers a rigorous method in the quest of new antibacterials. (-)-Roemerine is a plant alkaloid that has been reported to possess putative antibacterial activity against Escherichia coli, Bacillus subtilis, and Salmonella typhimurium. The aim of the present study was to characterize the activity of (-)-roemerine in Escherichia coli TB1 using proteomics tools. With (-)-roemerine treatment, we found limited permeability through the outer membrane and repression of transport proteins involved in carbohydrate metabolism, resulting in poor carbon source availability. The shortfall of intracellular carbon sources in turn led to impaired cell growth. The reduction in the abundance of proteins related to translational machinery, amino acid biosynthesis, and metabolism was accompanied by a nutrient-limited state. The latter finding could suggest a metabolic shutdown in E. coli cells. High osmolarity was clearly not one of the reasons of bacterial death by (-)-roemerine. These observations collectively attest to the promise of plant omics and profiling of putative drug candidates using proteomics tools. Omics-guided drug discovery deserves greater attention in mainstream pharmacology so as to better understand the plants' medicinal potentials.
dc.identifier.doi10.1089/omi.2015.0056
dc.identifier.eissn1557-8100
dc.identifier.issn1536-2310
dc.identifier.pubmed26230533
dc.identifier.urihttps://hdl.handle.net/11424/238176
dc.identifier.wosWOS:000359535100006
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INC
dc.relation.ispartofOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectOUTER-MEMBRANE PROTEINS
dc.subjectTYROSINE-PHOSPHATASE INHIBITORS
dc.subjectESCHERICHIA-COLI
dc.subjectAPORPHINE ALKALOIDS
dc.subjectISOQUINOLINE ALKALOIDS
dc.subjectNELUMBO-NUCIFERA
dc.subjectRESISTANCE
dc.subjectOMPX
dc.subjectEXPRESSION
dc.subjectOSMOREGULATION
dc.titleProteomics Evidence for the Activity of the Putative Antibacterial Plant Alkaloid (-)-Roemerine: Mainstreaming Omics-Guided Drug Discovery
dc.typearticle
dspace.entity.typePublication
local.avesis.id9b4b2c7b-a636-4118-922d-c33d18d5c305
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages12
oaire.citation.endPage489
oaire.citation.issue8
oaire.citation.startPage478
oaire.citation.titleOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
oaire.citation.volume19
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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